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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5247R-2 |
| Symbol |
Irbp |
Full Name |
Inverted repeat-binding protein |
| CG No |
CG5247 |
Old CG No |
CG5247 |
| Synonyms |
MUS309, mus309, DmBlm, Ku70, DmKu70, YPF1, dp70, IRBP, Ku, IRBP/Ku70, p70, YPF1beta, Ypf1b, CG5247, Irbp, ku70 |
| Accession No (Link to NCBI) |
NM_080512.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Lindström R, Lindholm P, Palgi M, Saarma M, Heino TI.
In vivo screening reveals interactions between Drosophila Manf and genes involved in the mitochondria and the ubiquinone synthesis pathway.
BMC Genet (2017) 18(1) 52
[
PubMed ID = 28578657
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAATCCGGAGAACGATGTGGAC |
| Primer Seq. 3' |
aatctagaggtaccGTGACATAGGCGATCTTGGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AATCCGGAGA ACGATGTGGA CCTGCTGTCT GGGTCCGAGG ACGAGGAGGA TGTGTCCATG
0061 AAGCGGGACT ACCATGGGCG CGAGGCCATT CTGTTCGTGG TAGACGCCAA TCTTCAGACA
0121 GCCGGCGTGG AGCGCCTGNT TGGAGGCACT GAACATCATC CGGACGGCCT TTATATCCGG
0181 ACTTCTGGTT AACGACAAGG ACCTCATCGG ACTCATCTTC GCCAACACCA AGCACAGTCC
0241 GCCGCCGCTG GAAGCCAGTG CATTGGACAA CATCGTAATG CCGGATAACT GCGCAGTGTT
0301 CTTGCCCCTT CGCCAACTAA CCAAACCCAT TGTGGAGCAC TATCTGGAAT TCATGGGCGG
0361 GGTGGAGACG CAGTTCGCCG ATGTGTATGG CCTGGCGGAA ACCCGATGGT CGCGGCAGGT
0421 TTGACCTTAT GATCCgGCTC TGCATCGAGA TGCTGGAAAA GTGCGGCAAG AAGCTAAACA
0481 ACGCCAAGAT CGCCTATGTC AC |
| in silico PCR Fragment |
0001 AATCCGGAGA ACGATGTGGA CCTGCTGTCT GGGTCCGAGG ACGAGGAGGA TGTGTCCATG
0061 AAGCGGGACT ACCATGGGCG CGAGGCCATT CTGTTCGTGG TAGACGCCAA TCTTCAGACA
0121 GCCGGCGTGG AGCGCCTG-T TGGAGGCACT GAACATCATC CGGACGGCCT TTATATCCGG
0181 ACTTCTGGTT AACGACAAGG ACCTCATCGG ACTCATCTTC GCCAACACCA AGCACAGTCC
0241 GCCGCCGCTG GAAGCCAGTG CATTGGACAA CATCGTAATG CCGGATAACT GCGCAGTGTT
0301 CTTGCCCCTT CGCCAACTAA CCAAACCCAT TGTGGAGCAC TATCTGGAAT TCATGGGCGG
0361 GGTGGAGACG CAGTTCGCCG ATGTGTATGG CCTGGCGG-A ACCCGATGGT CGCGGCAGGT
0421 TTGACCTTAT GATCCGGCTC TGCATCGAGA TGCTGGAAAA GTGCGGCAAG AAGCTAAACA
0481 ACGCCAAGAT CGCCTATGTC AC |
| Assemble Data |
5247R-2.IR_full 1 AATCCGGAGAACGATGTGGACCTGCTGTCTGGGTCCGAGGACGAGGAGGATGTGTCCATG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 1 AATCCGGAGAACGATGTGGACCTGCTGTCTGGGTCCGAGGACGAGGAGGATGTGTCCATG 60
5247R-2.IR_full 61 AAGCGGGACTACCATGGGCGCGAGGCCATTCTGTTCGTGGTAGACGCCAATCTTCAGACA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 61 AAGCGGGACTACCATGGGCGCGAGGCCATTCTGTTCGTGGTAGACGCCAATCTTCAGACA 120
5247R-2.IR_full 121 GCCGGCGTGGAGCGCCTGNTTGGAGGCACTGAACATCATCCGGACGGCCTTTATATCCGG 180
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 121 GCCGGCGTGGAGCGCCTG-TTGGAGGCACTGAACATCATCCGGACGGCCTTTATATCCGG 180
5247R-2.IR_full 181 ACTTCTGGTTAACGACAAGGACCTCATCGGACTCATCTTCGCCAACACCAAGCACAGTCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 181 ACTTCTGGTTAACGACAAGGACCTCATCGGACTCATCTTCGCCAACACCAAGCACAGTCC 240
5247R-2.IR_full 241 GCCGCCGCTGGAAGCCAGTGCATTGGACAACATCGTAATGCCGGATAACTGCGCAGTGTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 241 GCCGCCGCTGGAAGCCAGTGCATTGGACAACATCGTAATGCCGGATAACTGCGCAGTGTT 300
5247R-2.IR_full 301 CTTGCCCCTTCGCCAACTAACCAAACCCATTGTGGAGCACTATCTGGAATTCATGGGCGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 301 CTTGCCCCTTCGCCAACTAACCAAACCCATTGTGGAGCACTATCTGGAATTCATGGGCGG 360
5247R-2.IR_full 361 GGTGGAGACGCAGTTCGCCGATGTGTATGGCCTGGCGGAAACCCGATGGTCGCGGCAGGT 420
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
5247R-2.in silico 361 GGTGGAGACGCAGTTCGCCGATGTGTATGGCCTGGCGG-AACCCGATGGTCGCGGCAGGT 420
5247R-2.IR_full 421 TTGACCTTATGATCCGGCTCTGCATCGAGATGCTGGAAAAGTGCGGCAAGAAGCTAAACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5247R-2.in silico 421 TTGACCTTATGATCCGGCTCTGCATCGAGATGCTGGAAAAGTGCGGCAAGAAGCTAAACA 480
5247R-2.IR_full 481 ACGCCAAGATCGCCTATGTCAC 502
||||||||||||||||||||||
5247R-2.in silico 481 ACGCCAAGATCGCCTATGTCAC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_080512.2 |
CG5247-RA (Irbp), mRNA |
| 0.41 |
2 |
3 |
2 |
7 |
NM_141072.2 |
CG7177-RA (CG7177), mRNA |
| 0.2 |
1 |
3 |
4 |
10 |
NM_164429.1 |
CG31666-RC, transcript variant C (CG31666), mRNA |
| 0.2 |
1 |
3 |
4 |
10 |
NM_164428.2 |
CG31666-RB, transcript variant B (CG31666), mRNA |
| 0.2 |
1 |
3 |
4 |
10 |
NM_134766.3 |
CG31666-RA, transcript variant A (CG31666), mRNA |
| 0.2 |
1 |
3 |
4 |
10 |
NM_164430.2 |
CG31666-RD, transcript variant D (CG31666), mRNA |
| 0 |
0 |
5 |
2 |
2 |
NM_079789.1 |
CG8365-RA (E(spl)), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_143755.2 |
CG14813-RA (deltaCOP), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_143281.1 |
CG6066-RA (CG6066), mRNA |
| 0 |
0 |
2 |
7 |
4 |
NM_139778.2 |
CG10274-RA (CG10274), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079437.2 |
CG32211-RA (Taf6), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140174.4 |
CG7839-RA (CG7839), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_130725.2 |
CG15239-RA (CG15239), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_132313.1 |
CG15368-RA (CG15368), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_165678.2 |
CG1884-RB, transcript variant B (Not1), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_136653.3 |
CG1884-RA, transcript variant A (Not1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135393.2 |
CG13097-RA (CG13097), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080094.2 |
CG9696-RA, transcript variant A (dom), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166446.1 |
CG9696-RD, transcript variant D (dom), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176244.1 |
CG9696-RE, transcript variant E (dom), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136895.2 |
CG13176-RA (CG13176), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_078843.4 |
CG3479-RB, transcript variant B (osp), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165089.2 |
CG3479-RA, transcript variant A (osp), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_079782.2 |
CG8337-RA (malpha), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_078606.3 |
CG6146-RA, transcript variant A (Top1), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001014742.1 |
CG6146-RC, transcript variant C (Top1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166567.1 |
CG3682-RB, transcript variant B (PIP5K59B), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137885.2 |
CG3682-RA, transcript variant A (PIP5K59B), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170032.2 |
CG5248-RB, transcript variant B (loco), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001043101.1 |
CG3682-RC, transcript variant C (PIP5K59B), mRNA |
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