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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5208R-1 |
| Symbol |
Patr-1 |
Full Name |
Protein associated with topo II related - 1 |
| CG No |
CG5208 |
Old CG No |
CG5208 |
| Synonyms |
LD27979, BcDNA:LD27979, anon-WO0118547.282, Patr-1, CG5208 |
| Accession No (Link to NCBI) |
NM_142335.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
early pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGATGACTCGTTTTTCGGC |
| Primer Seq. 3' |
aatctagaggtaccCGGAACAAATGTTGGTTGTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA
0061 CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT
0121 GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG
0181 CGGAAGCGAC CCGCGGNAAT CTAGTGGAAA TACGGATTTT GCCGATCAGG GAAATGGTGC
0241 CTTCCTgNGA CACGCTAATC CGCTATCATC GGCGCCCTCT TCAAGCGGCT CCACTCCGGC
0301 CCCTTTGAAT GCCCCCTTCC GACAGTCTCG TCACATAGGG GACTCGGACT TGGAGCTAAA
0361 CATTTCCTCC ATGAAGTTGG ACGACATGGA CCTGTCCTCT TTCACCACTG ATAGCGAAGC
0421 CGGTGGGCTG AGCAACCGCA TTAAGCTGGA CTCCGGGGTC TGGGGATCAC AGCCGTTTCC
0481 CAACAACCAA CATTTGTTCC G |
| in silico PCR Fragment |
0001 ATGGATGACT CGTTTTTCGG CTTTGACACG AATCTACCGG ACGAAGATGG TGGCGATGGA
0061 CGCATCGTCG AGGCGGAATA CGATGCCCTC AATGACGAAA CCTTTGGATC CGCTATAAAT
0121 GGCGACTGGG AGGAAGCCCA TGAGACGATG GTACGCCTGG GCGGGAATGG CGAAAGGGTG
0181 CGGAAGCGAC CCGCGG-AAT CTAGTGGAAA TACGGATTTT GCCGATCAGG GAAATGGTGC
0241 CTTCCTGCGA CACGCTAATC CGCTATCATC GGCGCCCTCT TCAAGCGGCT CCACTCCGGC
0301 CCCTTTGAAT GCCCCCTTCC GACAGTCTCG TCACATAGGG GACTCGGACT TGGAGCTAAA
0361 CATTTCCTCC ATGAAGTTGG ACGACATGGA CCTGTCCTCT TTCACCACTG ATAGCGAAGC
0421 CGGTGGGCTG AGCAACCGCA TTAAGCTGGA CTCCGGGGTC TGGGGATCAC AGCCGTTTCC
0481 CAACAACCAA CATTTGTTCC G |
| Assemble Data |
5208R-1.IR_full 1 ATGGATGACTCGTTTTTCGGCTTTGACACGAATCTACCGGACGAAGATGGTGGCGATGGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 1 ATGGATGACTCGTTTTTCGGCTTTGACACGAATCTACCGGACGAAGATGGTGGCGATGGA 60
5208R-1.IR_full 61 CGCATCGTCGAGGCGGAATACGATGCCCTCAATGACGAAACCTTTGGATCCGCTATAAAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 61 CGCATCGTCGAGGCGGAATACGATGCCCTCAATGACGAAACCTTTGGATCCGCTATAAAT 120
5208R-1.IR_full 121 GGCGACTGGGAGGAAGCCCATGAGACGATGGTACGCCTGGGCGGGAATGGCGAAAGGGTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 121 GGCGACTGGGAGGAAGCCCATGAGACGATGGTACGCCTGGGCGGGAATGGCGAAAGGGTG 180
5208R-1.IR_full 181 CGGAAGCGACCCGCGGNAATCTAGTGGAAATACGGATTTTGCCGATCAGGGAAATGGTGC 240
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 181 CGGAAGCGACCCGCGG-AATCTAGTGGAAATACGGATTTTGCCGATCAGGGAAATGGTGC 240
5208R-1.IR_full 241 CTTCCTGNGACACGCTAATCCGCTATCATCGGCGCCCTCTTCAAGCGGCTCCACTCCGGC 300
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 241 CTTCCTGCGACACGCTAATCCGCTATCATCGGCGCCCTCTTCAAGCGGCTCCACTCCGGC 300
5208R-1.IR_full 301 CCCTTTGAATGCCCCCTTCCGACAGTCTCGTCACATAGGGGACTCGGACTTGGAGCTAAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 301 CCCTTTGAATGCCCCCTTCCGACAGTCTCGTCACATAGGGGACTCGGACTTGGAGCTAAA 360
5208R-1.IR_full 361 CATTTCCTCCATGAAGTTGGACGACATGGACCTGTCCTCTTTCACCACTGATAGCGAAGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 361 CATTTCCTCCATGAAGTTGGACGACATGGACCTGTCCTCTTTCACCACTGATAGCGAAGC 420
5208R-1.IR_full 421 CGGTGGGCTGAGCAACCGCATTAAGCTGGACTCCGGGGTCTGGGGATCACAGCCGTTTCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5208R-1.in silico 421 CGGTGGGCTGAGCAACCGCATTAAGCTGGACTCCGGGGTCTGGGGATCACAGCCGTTTCC 480
5208R-1.IR_full 481 CAACAACCAACATTTGTTCCG 501
|||||||||||||||||||||
5208R-1.in silico 481 CAACAACCAACATTTGTTCCG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
4 |
6 |
NM_142335.2 |
CG5208-RA (CG5208), mRNA |
| 0.2 |
1 |
2 |
3 |
2 |
NM_137994.2 |
CG5554-RA (CG5554), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_141014.1 |
CG10588-RA (CG10588), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143345.2 |
CG4951-RA (CG4951), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206140.1 |
CG8912-RC, transcript variant C (Psi), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_057775.3 |
CG8912-RB, transcript variant B (Psi), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_166200.2 |
CG8912-RA, transcript variant A (Psi), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_079845.2 |
CG7951-RA (sima), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_001015300.1 |
CG40119-PA.3 (CG40119), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_136055.2 |
CG10376-RA (CG10376), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137773.1 |
CG13493-RA (comr), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057538.2 |
CG7435-RA (Arf84F), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142334.1 |
CG3995-RA (CG3995), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001015173.1 |
CG41065-PA.3 (CG41065), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141237.1 |
CG14655-RA (CG14655), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167277.1 |
CG11105-RA, transcript variant A (CG11105), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132471.1 |
CG11105-RB, transcript variant B (CG11105), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080498.2 |
CG5942-RA, transcript variant A (brm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168640.1 |
CG5942-RD, transcript variant D (brm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080497.4 |
CG5942-RC, transcript variant C (brm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168641.1 |
CG5942-RB, transcript variant B (brm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166703.1 |
CG2727-RC, transcript variant C (emp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143739.2 |
CG18321-RA (miple2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166702.1 |
CG2727-RB, transcript variant B (emp), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_167190.1 |
CG15316-RB, transcript variant B (CG15316), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_132338.1 |
CG15316-RA, transcript variant A (CG15316), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132200.1 |
CG1531-RB (CG1531), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132410.2 |
CG2124-RA (CG2124), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_137732.2 |
CG9754-RA (CG9754), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141723.1 |
CG6208-RA (CG6208), mRNA |
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