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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5198R-2 
 Symbol CG5198  Full Name CG5198 
 CG No CG5198  Old CG No CG5198 
 Synonyms CG5198 
 Accession No (Link to NCBI) NM_135560.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGCGAGCAAAAGAAAGCAC 
 Primer Seq. 3' 
aatctagaggtaccCTCTGAAGGGTTGTTAGGACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGCGAGCA AAAGAAAGCA CCANGCATCC CAGAAAGTGA AAGAGGAATC CTTCAAGAAA 
0061 CACACGCTTG ACTCCGATGA AGAGGATTCT GACGACTACG AAAGGGAATA CCTCAATGAT 
0121 AGCGACATTG AGGGCGGCGA GGAGGGCGTG GCAAAAGTGG AAGACGATGT GAAGGTAACA 
0181 CCCTTCAATA TGAAGGAAGA ACTGGAGGAG GGACACTTCG ACAAAGATGG TCACTACCAT 
0241 TGGAACAAGG AGACGGAAGC AAAGGACAAT TGGTTGGACA ACATAGACTG GGTTAAGATT 
0301 GGCACACAGA AGAACGCCTT CGACCCGGCA AAAGATGAAG AAAACTCCTC CGATGAGGAG 
0361 AAGAACGAAC CCGTGGNCAA GGCCTTTAAC TTGTCCATGA ACCTCATGAA AATGGTCGAG 
0421 TTTATGAAAC CGGGAGAGAC TGTCAAGATG ACCCTGCAGC GTCTGGGTAA GCAGCGCCCC 
0481 GTCCTAATAA CCCTTCAGAG  
 in silico PCR Fragment 
0001 ATGGCGAGCA AAAGAAAGCA CCAAGCATCC CAGAAAGTGA AAGAGGAATC CTTCAAGAAA 
0061 CACACGCTTG ACTCCGATGA AGAGGATTCT GACGACTACG AAAGGGAATA CCTCAATGAT 
0121 AGCGACATTG AGGGCGGCGA GGAGGGCGTG GCAAAAGTGG AAGACGATGT GAAGGTAACA 
0181 CCCTTCAATA TGAAGGAAGA ACTGGAGGAG GGACACTTCG ACAAAGATGG TCACTACCAT 
0241 TGGAACAAGG AGACGGAAGC AAAGGACAAT TGGTTGGACA ACATAGACTG GGTTAAAATT 
0301 GGCACACAGA AGAACGCCTT CGACCCGGCA AAAGATGAAG AAAACTCTTC CGATGAGGAG 
0361 AAGAACGAAC CCGTGGGCAA GGCCTTTAAC TTGTCCATGA ACCTCATGAA AATGGTCGAG 
0421 TTTATGAAAC CGGGAGAGAC TGTCAAGATG ACCCTGCAGC GTCTGGGTAA GCAGCGCCCC 
0481 GTCCTAACAA CCCTTCAGAG  
 Assemble Data 

5198R-2.IR_full       1   ATGGCGAGCAAAAGAAAGCACCANGCATCCCAGAAAGTGAAAGAGGAATCCTTCAAGAAA 60
                          ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
5198R-2.in silico     1   ATGGCGAGCAAAAGAAAGCACCAAGCATCCCAGAAAGTGAAAGAGGAATCCTTCAAGAAA 60


5198R-2.IR_full       61  CACACGCTTGACTCCGATGAAGAGGATTCTGACGACTACGAAAGGGAATACCTCAATGAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5198R-2.in silico     61  CACACGCTTGACTCCGATGAAGAGGATTCTGACGACTACGAAAGGGAATACCTCAATGAT 120


5198R-2.IR_full       121 AGCGACATTGAGGGCGGCGAGGAGGGCGTGGCAAAAGTGGAAGACGATGTGAAGGTAACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5198R-2.in silico     121 AGCGACATTGAGGGCGGCGAGGAGGGCGTGGCAAAAGTGGAAGACGATGTGAAGGTAACA 180


5198R-2.IR_full       181 CCCTTCAATATGAAGGAAGAACTGGAGGAGGGACACTTCGACAAAGATGGTCACTACCAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5198R-2.in silico     181 CCCTTCAATATGAAGGAAGAACTGGAGGAGGGACACTTCGACAAAGATGGTCACTACCAT 240


5198R-2.IR_full       241 TGGAACAAGGAGACGGAAGCAAAGGACAATTGGTTGGACAACATAGACTGGGTTAAGATT 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
5198R-2.in silico     241 TGGAACAAGGAGACGGAAGCAAAGGACAATTGGTTGGACAACATAGACTGGGTTAAAATT 300


5198R-2.IR_full       301 GGCACACAGAAGAACGCCTTCGACCCGGCAAAAGATGAAGAAAACTCCTCCGATGAGGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
5198R-2.in silico     301 GGCACACAGAAGAACGCCTTCGACCCGGCAAAAGATGAAGAAAACTCTTCCGATGAGGAG 360


5198R-2.IR_full       361 AAGAACGAACCCGTGGNCAAGGCCTTTAACTTGTCCATGAACCTCATGAAAATGGTCGAG 420
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
5198R-2.in silico     361 AAGAACGAACCCGTGGGCAAGGCCTTTAACTTGTCCATGAACCTCATGAAAATGGTCGAG 420


5198R-2.IR_full       421 TTTATGAAACCGGGAGAGACTGTCAAGATGACCCTGCAGCGTCTGGGTAAGCAGCGCCCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5198R-2.in silico     421 TTTATGAAACCGGGAGAGACTGTCAAGATGACCCTGCAGCGTCTGGGTAAGCAGCGCCCC 480


5198R-2.IR_full       481 GTCCTAATAACCCTTCAGAG 500
                          ||||||| ||||||||||||
5198R-2.in silico     481 GTCCTAACAACCCTTCAGAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135560.2  CG5198-RA (CG5198), mRNA 
0   0  3  3  3  NM_141341.2  CG1208-RA (CG1208), mRNA 
0   0  2  3  5  NM_131979.1  CG5062-RA (CG5062), mRNA 
0   0  2  3  2  NM_176174.1  CG12864-RB, transcript variant B (Su(var)2-HP2), mRNA 
0   0  2  3  2  NM_137128.2  CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA 
0   0  2  2  2  NM_001014621.1  CG8651-RE, transcript variant E (trx), mRNA 
0   0  2  2  2  NM_134282.1  CG8651-RA, transcript variant A (trx), mRNA 
0   0  2  2  2  NM_057422.2  CG8651-RB, transcript variant B (trx), mRNA 
0   0  2  2  2  NM_057421.2  CG8651-RD, transcript variant D (trx), mRNA 
0   0  2  2  2  NM_134281.1  CG8651-RC, transcript variant C (trx), mRNA 
0   0  1  4  9  NM_143281.1  CG6066-RA (CG6066), mRNA 
0   0  1  4  5  NM_142570.2  CG7535-RA, transcript variant A (GluClalpha), mRNA 
0   0  1  3  6  NM_137895.1  CG3800-RA (CG3800), mRNA 
0   0  1  3  5  NM_001014641.1  CG7535-RC, transcript variant C (GluClalpha), mRNA 
0   0  1  3  3  NM_142718.1  CG6353-RA (CG6353), mRNA 
0   0  1  3  2  NM_169873.1  CG7535-RB, transcript variant B (GluClalpha), mRNA 
0   0  1  3  2  NM_001038972.1  CG7535-RD, transcript variant D (GluClalpha), mRNA 
0   0  1  2  4  NM_206262.1  CG12734-RB, transcript variant B (CG12734), mRNA 
0   0  1  2  4  NM_139523.1  CG12734-RA, transcript variant A (CG12734), mRNA 
0   0  1  2  4  NM_078529.3  CG18009-RD, transcript variant D (Trf2), mRNA 
0   0  1  2  4  NM_206357.2  CG33261-RD, transcript variant D (Trl), mRNA 
0   0  1  2  4  NM_206356.2  CG33261-RB, transcript variant B (Trl), mRNA 
0   0  1  2  4  NM_135750.1  CG16800-RA (CG16800), mRNA 
0   0  1  2  4  NM_206654.1  CG18009-RA, transcript variant A (Trf2), mRNA 
0   0  1  2  4  NM_137623.2  CG9143-RA (CG9143), mRNA 
0   0  1  2  3  NM_136495.2  CG2910-RB, transcript variant B (nito), mRNA 
0   0  1  2  3  NM_165579.1  CG2910-RA, transcript variant A (nito), mRNA 
0   0  1  2  2  NM_134989.2  CG2910-RA, transcript variant A (nito), mRNA, ubiquitously expressed CG15437-RA (morgue), mRNA 
0   0  1  2  2  NM_001038926.1  CG33261-RI, transcript variant I (Trl), mRNA 
0   0  1  2  2  NM_206358.2  CG33261-RA, transcript variant A (Trl), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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