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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5178R-1 |
| Symbol |
Act88F |
Full Name |
Actin 88F |
| CG No |
CG5178 |
Old CG No |
CG5178 |
| Synonyms |
Actin, actin, E, act88F, CG5178, Act(88F), act 88F, Act88-F, Ifm(3)7, rsd, Act88F |
| Accession No (Link to NCBI) |
NM_079643.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCCGGTCGTACCACCGGTAT |
| Primer Seq. 3' |
aatctagaggtaccTACCGCCGGACAGCACAGAGT |
| Predicted Fragment Size |
476 |
| IR fragment full Seq |
0001 CTCCgGTCGT ACCACCGGTA TTGTGCTGGA CTCCGGCGAT GGTGTCTCCC ACACCGTGCC
0061 CATCTATGAG GGCTTcgcCC NGCNCCCACG CCATTCTGCG TCTGGATCTG GCTGGTCGCG
0121 ATCTGACCGA TTACCTGATG AAGATCCTGA CGGAGCGCGG CTACAGCNTT CACCACCACC
0181 GCCGAGCGTG AGATCGTGCG CGACATCAAG GAGAAGCTGT GCTACGTGGC TCTGGACTTC
0241 GAGCAGGAGA TGGCCACCGC TGCCGCCTCC ACCTCGCTGG AGAAGTCGTA CGAGTTGCCT
0301 GACGGCCAGG TGATCACCAT TGGCAANCGA GCGCTTCCGC TGCCCCGAGG CTCTGTTCCA
0361 GCCCTCGTTC CTGGGCATGG AGTCGTGCGG CATCCACGAG ACCGTCTACA ACTCGATCAT
0421 GAAGTGCGAC GTGGACATCC GCAAGGATCT GTATGCCAAC TCTGTGCTGT NCGGCGGTA |
| in silico PCR Fragment |
0001 CTCCGGTCGT ACCACCGGTA TTGTGCTGGA CTCCGGCGAT GGTGTCTCCC ACACCGTGCC
0061 CATCTATGAG GGCTTCGCCC TGC-CCCACG CCATTCTGCG TCTGGATCTG GCTGGTCGCG
0121 ATCTGACCGA TTACCTGATG AAGATCCTGA CGGAGCGCGG CTACAGC-TT CACCACCACC
0181 GCCGAGCGTG AGATCGTGCG CGACATCAAG GAGAAGCTGT GCTACGTGGC TCTGGACTTC
0241 GAGCAGGAGA TGGCCACCGC TGCCGCCTCC ACCTCGCTGG AGAAGTCGTA CGAGTTGCCT
0301 GACGGCCAGG TGATCACCAT TGGCAA-CGA GCGCTTCCGC TGCCCCGAGG CTCTGTTCCA
0361 GCCCTCGTTC CTGGGCATGG AGTCGTGCGG CATCCACGAG ACCGTCTACA ACTCGATCAT
0421 GAAGTGCGAC GTGGACATCC GCAAGGATCT GTATGCCAAC TCTGTGCTGT CCGGCGGTA |
| Assemble Data |
5178R-1.IR_full 1 CTCCGGTCGTACCACCGGTATTGTGCTGGACTCCGGCGATGGTGTCTCCCACACCGTGCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico 1 CTCCGGTCGTACCACCGGTATTGTGCTGGACTCCGGCGATGGTGTCTCCCACACCGTGCC 60
5178R-1.IR_full 61 CATCTATGAGGGCTTCGCCCNGCNCCCACGCCATTCTGCGTCTGGATCTGGCTGGTCGCG 120
|||||||||||||||||||| || ||||||||||||||||||||||||||||||||||||
5178R-1.in silico 61 CATCTATGAGGGCTTCGCCCTGC-CCCACGCCATTCTGCGTCTGGATCTGGCTGGTCGCG 120
5178R-1.IR_full 121 ATCTGACCGATTACCTGATGAAGATCCTGACGGAGCGCGGCTACAGCNTTCACCACCACC 180
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
5178R-1.in silico 121 ATCTGACCGATTACCTGATGAAGATCCTGACGGAGCGCGGCTACAGC-TTCACCACCACC 180
5178R-1.IR_full 181 GCCGAGCGTGAGATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTGGCTCTGGACTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico 181 GCCGAGCGTGAGATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTGGCTCTGGACTTC 240
5178R-1.IR_full 241 GAGCAGGAGATGGCCACCGCTGCCGCCTCCACCTCGCTGGAGAAGTCGTACGAGTTGCCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico 241 GAGCAGGAGATGGCCACCGCTGCCGCCTCCACCTCGCTGGAGAAGTCGTACGAGTTGCCT 300
5178R-1.IR_full 301 GACGGCCAGGTGATCACCATTGGCAANCGAGCGCTTCCGCTGCCCCGAGGCTCTGTTCCA 360
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
5178R-1.in silico 301 GACGGCCAGGTGATCACCATTGGCAA-CGAGCGCTTCCGCTGCCCCGAGGCTCTGTTCCA 360
5178R-1.IR_full 361 GCCCTCGTTCCTGGGCATGGAGTCGTGCGGCATCCACGAGACCGTCTACAACTCGATCAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5178R-1.in silico 361 GCCCTCGTTCCTGGGCATGGAGTCGTGCGGCATCCACGAGACCGTCTACAACTCGATCAT 420
5178R-1.IR_full 421 GAAGTGCGACGTGGACATCCGCAAGGATCTGTATGCCAACTCTGTGCTGTNCGGCGGTA 479
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
5178R-1.in silico 421 GAAGTGCGACGTGGACATCCGCAAGGATCTGTATGCCAACTCTGTGCTGTCCGGCGGTA 479
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
458 |
0 |
0 |
0 |
NM_079643.1 |
CG5178-RA (Act88F), mRNA |
| 19.65 |
90 |
94 |
184 |
70 |
NM_079486.3 |
CG7478-RA (Act79B), mRNA |
| 10.48 |
48 |
119 |
134 |
78 |
NM_001014726.1 |
CG4027-RC, transcript variant C (Act5C), mRNA |
| 10.48 |
48 |
119 |
134 |
78 |
NM_001014725.1 |
CG4027-RD, transcript variant D (Act5C), mRNA |
| 10.48 |
48 |
119 |
134 |
78 |
NM_167053.1 |
CG4027-RA, transcript variant A (Act5C), mRNA |
| 10.48 |
48 |
119 |
134 |
78 |
NM_078497.2 |
CG4027-RB, transcript variant B (Act5C), mRNA |
| 9.82 |
45 |
150 |
125 |
82 |
NM_057743.3 |
CG18290-RA, transcript variant A (Act87E), mRNA |
| 9.82 |
45 |
150 |
125 |
82 |
NM_169525.1 |
CG18290-RB, transcript variant B (Act87E), mRNA |
| 8.73 |
40 |
50 |
157 |
119 |
NM_078901.2 |
CG12051-RA (Act42A), mRNA |
| 7.64 |
35 |
137 |
106 |
105 |
NM_166414.1 |
CG10067-RB, transcript variant B (Act57B), mRNA |
| 7.64 |
35 |
137 |
106 |
105 |
NM_079076.3 |
CG10067-RA, transcript variant A (Act57B), mRNA |
| 0.65 |
3 |
3 |
3 |
22 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0.65 |
3 |
3 |
3 |
22 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0.65 |
3 |
3 |
3 |
22 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_144354.2 |
CG6987-RA (SF2), mRNA |
| 0 |
0 |
3 |
5 |
10 |
NM_132320.2 |
CG12124-RA (CG12124), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001031917.1 |
CG1414-RB, transcript variant B (bbx), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001031918.1 |
CG1410-RA, transcript variant A (waw), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_001031916.1 |
CG1414-RC, transcript variant C (bbx), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_139766.1 |
CG6619-RA (CG6619), mRNA |
| 0 |
0 |
2 |
20 |
46 |
NM_057689.1 |
CG5409-RA (Arp53D), mRNA |
| 0 |
0 |
2 |
8 |
8 |
NM_078648.2 |
CG9901-RA (Arp14D), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_057862.3 |
CG17246-RA, transcript variant A (Scs-fp), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_166343.1 |
CG17246-RB, transcript variant B (Scs-fp), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_166344.1 |
CG17246-RC, transcript variant C (Scs-fp), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_136415.1 |
CG12835-RA (CG12835), mRNA |
| 0 |
0 |
2 |
3 |
13 |
NM_168755.1 |
CG8127-RB, transcript variant B (Eip75B), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_167020.1 |
CG6986-RB, transcript variant B (CG6986), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_001014724.1 |
CG6986-RC, transcript variant C (CG6986), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_131955.1 |
CG6986-RA, transcript variant A (CG6986), mRNA |
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