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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5096R-2 
 Symbol CG5096  Full Name CG5096 
 CG No CG5096  Old CG No CG5096 
 Synonyms CT16355, CG5096 
 Accession No (Link to NCBI) NM_164918.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATCTGCATAGCTTGGATGCG 
 Primer Seq. 3' 
aatctagaggtaccTGGCCACCAGTTACGTTCTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GATCTGCATA GCTTGGATGC GGATCTGTTT GAACACATTC CCCACATTGA GGAACTCGTT 
0061 CTCTGCTCAA ATAGTTTTCA TGTCATCGAT CAACTTTCAG AAACAGCCAT TTCCGGCTTA 
0121 CAATCGTTGA AGATCTTGGA TGTCTCTTAT ATGGAAATCG ATGACTTGCC GGATACAATT 
0181 CTACATGGTC CCCGAGATCT TGAGATCTTT ATAGCAGCTG GCAATCTGTT TAATCAATTG 
0241 CCAAAGGCCC TGAAATATGC GACGAACTTG ACCAGCCTGG TCTTAAACGA AAATCCCATC 
0301 GAAAATTTGA TTGGCGACAA TGTATTTCCA CCTTTGACCA AATTGACCCA CCTCAGCATG 
0361 ACTTTTATGT CCAAGTTGTA CAAAATTGGG CCAGGAGCAT TTAGTGAACT GCAAAGTTTA 
0421 ACTGAACTGA TATTGTCCGA CAACAAACTG CTAAATGAAA TTGATGAGGA GGCTCTATCC 
0481 AAGAACGTAA CTGGTGGCCA  
 in silico PCR Fragment 
0001 GATCTGCATA GCTTGGATGC GGATCTGTTT GAACACATTC CCCACATTGA GGAACTCGTT 
0061 CTCTGCTCAA ATAGTTTTCA TGTCATCGAT CAACTTTCAG AAACAGCCAT TTCCGGCTTA 
0121 CAATCGTTGA AGATCTTGGA TGTCTCTTAT ATGGAAATCG ATGACTTGCC GGATACAATT 
0181 CTACATGGTC CCCGAGATCT TGAGATCTTT ATAGCAGCTG GCAATCTGTT TAATCAATTG 
0241 CCAAAGGCCC TGAAATATGC GACGAACTTG ACCAGCCTGG TCTTAAACGA AAATCCCATC 
0301 GAAAATTTGA TTGGCGACAA TGTATTTCCA CCTTTGACCA AATTGACCCA CCTCAGCATG 
0361 ACTTTTATGT CCAAGTTGTA CAAAATTGGG CCAGGAGCAT TTAGTGAACT GCAAAGTTTA 
0421 ACTGAACTGA TATTGTCCGA CAACAAACTG CTAAATGAAA TTGATGAGGA GGCTCTATCC 
0481 AAGAACGTAA CTGGTGGCCA  
 Assemble Data 

5096R-2.IR_full       1   GATCTGCATAGCTTGGATGCGGATCTGTTTGAACACATTCCCCACATTGAGGAACTCGTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     1   GATCTGCATAGCTTGGATGCGGATCTGTTTGAACACATTCCCCACATTGAGGAACTCGTT 60


5096R-2.IR_full       61  CTCTGCTCAAATAGTTTTCATGTCATCGATCAACTTTCAGAAACAGCCATTTCCGGCTTA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     61  CTCTGCTCAAATAGTTTTCATGTCATCGATCAACTTTCAGAAACAGCCATTTCCGGCTTA 120


5096R-2.IR_full       121 CAATCGTTGAAGATCTTGGATGTCTCTTATATGGAAATCGATGACTTGCCGGATACAATT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     121 CAATCGTTGAAGATCTTGGATGTCTCTTATATGGAAATCGATGACTTGCCGGATACAATT 180


5096R-2.IR_full       181 CTACATGGTCCCCGAGATCTTGAGATCTTTATAGCAGCTGGCAATCTGTTTAATCAATTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     181 CTACATGGTCCCCGAGATCTTGAGATCTTTATAGCAGCTGGCAATCTGTTTAATCAATTG 240


5096R-2.IR_full       241 CCAAAGGCCCTGAAATATGCGACGAACTTGACCAGCCTGGTCTTAAACGAAAATCCCATC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     241 CCAAAGGCCCTGAAATATGCGACGAACTTGACCAGCCTGGTCTTAAACGAAAATCCCATC 300


5096R-2.IR_full       301 GAAAATTTGATTGGCGACAATGTATTTCCACCTTTGACCAAATTGACCCACCTCAGCATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     301 GAAAATTTGATTGGCGACAATGTATTTCCACCTTTGACCAAATTGACCCACCTCAGCATG 360


5096R-2.IR_full       361 ACTTTTATGTCCAAGTTGTACAAAATTGGGCCAGGAGCATTTAGTGAACTGCAAAGTTTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     361 ACTTTTATGTCCAAGTTGTACAAAATTGGGCCAGGAGCATTTAGTGAACTGCAAAGTTTA 420


5096R-2.IR_full       421 ACTGAACTGATATTGTCCGACAACAAACTGCTAAATGAAATTGATGAGGAGGCTCTATCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5096R-2.in silico     421 ACTGAACTGATATTGTCCGACAACAAACTGCTAAATGAAATTGATGAGGAGGCTCTATCC 480


5096R-2.IR_full       481 AAGAACGTAACTGGTGGCCA 500
                          ||||||||||||||||||||
5096R-2.in silico     481 AAGAACGTAACTGGTGGCCA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_164918.1  CG5096-RA (CG5096), mRNA 
0   0  2  2  4  NM_136340.2  CG7897-RA (gp210), mRNA 
0   0  2  2  3  NM_166335.2  CG7097-RA, transcript variant A (CG7097), mRNA 
0   0  2  2  3  NM_137555.2  CG7097-RB, transcript variant B (CG7097), mRNA 
0   0  1  8  3  NM_206678.1  CG33175-RA, transcript variant A (spri), mRNA 
0   0  1  4  3  NM_205957.1  CG33304-RA (rho-5), mRNA 
0   0  1  2  3  NM_142445.1  CG7146-RA (CG7146), mRNA 
0   0  1  2  3  NM_170576.1  CG1945-RC, transcript variant C (faf), mRNA 
0   0  1  2  3  NM_079873.3  CG1945-RA, transcript variant A (faf), mRNA 
0   0  0  6  6  NM_057257.3  CG7664-RA (crp), mRNA 
0   0  0  5  4  NM_166734.1  CG32006-RA (CG32006), mRNA 
0   0  0  4  6  NM_138088.2  CG11414-RA (CG11414), mRNA 
0   0  0  4  4  NM_139532.1  CG14967-RA (CG14967), mRNA 
0   0  0  4  3  NM_057926.2  CG10533-RA (Lcp65Af), mRNA 
0   0  0  3  8  NM_080003.2  CG5012-RA (mRpL12), mRNA 
0   0  0  3  7  NM_136017.2  CG15151-RA (PFE), mRNA 
0   0  0  3  7  NM_080306.2  CG3443-RB (pcx), mRNA 
0   0  0  3  5  NM_167245.1  CG32672-RA (Atg8a), mRNA 
0   0  0  3  5  NM_142930.2  CG10198-RA (Nup98), mRNA 
0   0  0  3  4  NM_142316.1  CG14906-RA (CG14906), mRNA 
0   0  0  3  4  NM_143802.2  CG8013-RA, transcript variant A (Su(z)12), mRNA 
0   0  0  3  4  NM_168826.1  CG8013-RB, transcript variant B (Su(z)12), mRNA 
0   0  0  3  4  NM_078583.2  CG2559-RA (Lsp1alpha), mRNA 
0   0  0  3  3  NM_169956.1  CG31182-RA (CG31182), mRNA 
0   0  0  3  3  NM_140056.2  CG3891-RA (CG3891), mRNA 
0   0  0  3  3  NM_141014.1  CG10588-RA (CG10588), mRNA 
0   0  0  3  2  NM_169690.1  CG8884-RB, transcript variant B (Sap47), mRNA 
0   0  0  3  2  NM_139816.1  CG8641-RA (CG8641), mRNA 
0   0  0  3  2  NM_206420.1  CG33291-RA (CG33291), mRNA 
0   0  0  3  2  NM_079647.2  CG8884-RF, transcript variant F (Sap47), mRNA 

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