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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5083R-2 |
| Symbol |
Rbf2 |
Full Name |
Retinoblastoma-family protein 2 |
| CG No |
CG5083 |
Old CG No |
CG5083 |
| Synonyms |
RBF2, rbf2, CG5083, Rbf2 |
| Accession No (Link to NCBI) |
NM_079648.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTCAGCTGCGAGCAATTGGAG |
| Primer Seq. 3' |
aatctagaggtaccCGAATGACCAGGAAGAGCAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTCAGCTGCG AGCAATTGGA GCTGGAANCG NGNAATTCAG CAAAGcGNTC TGTCCACCTA
0061 CCATCGCTTG GATGCGGTCA ACGGGCTGTC CACCAGCGAG GCAGATGcCC AGGAGTGGCT
0121 GTNTTGCGCC GTCTACAGCG AACTGCAGCg NTCGAAGATG CGcGNTATTA GGGAGTCCAT
0181 CAACGAGGCA ACACGATTCG GTGGCCAAGA ACTGCTGCTG GAACGTGTCA CTAACNCGTC
0241 TGTCTGCGCA GCTTTAAGAT GAACGTGTCC CAGTTTCTAC GCCGCATGGA GNACTGGAAT
0301 TGGCTGACCC AAAACGAGAA CACTTTCCAG CTGGAGGTTG AGGAAcTGCG TTGTCGACTT
0361 GGTATTACTT CGACGCTGCT GCGGCATTAT AAGCACANCT TTCGGAGCCT GTTCGTTCAC
0421 CCCGGCAAGG GTgNGGACCC GGGTGCCGCG AATCACTACC AAGCGCTGTA TGAGTTCGGT
0481 TGGTTGCTCT TCCTGGTCAT TCG |
| in silico PCR Fragment |
0001 GTCAGCTGCG AGCAATTGGA GCTGGAAGCG AG-AATTCAG CAAAGCGCTC TGTCCACCTA
0061 CCATCGCTTG GATGCGGTCA ACGGGCTGTC CACCAGCGAG GCAGATGCCC AGGAGTGGCT
0121 GTGTTGCGCC GTCTACAGCG AACTGCAGCG CTCGAAGATG CGCGATATTA GGGAGTCCAT
0181 CAACGAGGCA A-ACGATTCG GTGGCCAAGA ACTGCTGCTG GAACGTGTCA CTAACCCGTC
0241 TG-CTGCGCA GCTTTAAGAT GAACGTGTCC CAGTTTCTAC GCCGCATGGA GCACTGGAAT
0301 TGGCTGACCC AAAACGAGAA CACTTTCCAG CTGGAGGTTG AGGAACTGCG TTGTCGACTT
0361 GGTATTACTT CGACGCTGCT GCGGCATTAT AAGCACATCT TTCGGAGCCT GTTCGTTCAC
0421 CCCGGCAAGG GTGCGGACCC GGGTGCCGCG AATCACTACC AAGCGCTGTA TGAGTTCGGT
0481 TGGTTGCTCT TCCTGGTCAT TCG |
| Assemble Data |
5083R-2.IR_full 1 TCAGCTGCGAGCAATTGGAGCTGGAANCGNGNAATTCAGCAAAGCGNTCTGTCCACCTA 59
|||||||||||||||||||||||||| || | |||||||||||||| ||||||||||||
5083R-2.in silico 1 TCAGCTGCGAGCAATTGGAGCTGGAAGCGAG-AATTCAGCAAAGCGCTCTGTCCACCTA 59
5083R-2.IR_full 61 CCATCGCTTGGATGCGGTCAACGGGCTGTCCACCAGCGAGGCAGATGCCCAGGAGTGGCT 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5083R-2.in silico 61 CCATCGCTTGGATGCGGTCAACGGGCTGTCCACCAGCGAGGCAGATGCCCAGGAGTGGCT 119
5083R-2.IR_full 121 GTNTTGCGCCGTCTACAGCGAACTGCAGCGNTCGAAGATGCGCGNTATTAGGGAGTCCAT 179
|| ||||||||||||||||||||||||||| ||||||||||||| |||||||||||||||
5083R-2.in silico 121 GTGTTGCGCCGTCTACAGCGAACTGCAGCGCTCGAAGATGCGCGATATTAGGGAGTCCAT 179
5083R-2.IR_full 181 CAACGAGGCAACACGATTCGGTGGCCAAGAACTGCTGCTGGAACGTGTCACTAACNCGTC 239
||||||||||| ||||||||||||||||||||||||||||||||||||||||||| ||||
5083R-2.in silico 181 CAACGAGGCAA-ACGATTCGGTGGCCAAGAACTGCTGCTGGAACGTGTCACTAACCCGTC 239
5083R-2.IR_full 241 TGTCTGCGCAGCTTTAAGATGAACGTGTCCCAGTTTCTACGCCGCATGGAGNACTGGAAT 299
|| |||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
5083R-2.in silico 241 TG-CTGCGCAGCTTTAAGATGAACGTGTCCCAGTTTCTACGCCGCATGGAGCACTGGAAT 299
5083R-2.IR_full 301 TGGCTGACCCAAAACGAGAACACTTTCCAGCTGGAGGTTGAGGAACTGCGTTGTCGACTT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5083R-2.in silico 301 TGGCTGACCCAAAACGAGAACACTTTCCAGCTGGAGGTTGAGGAACTGCGTTGTCGACTT 359
5083R-2.IR_full 361 GGTATTACTTCGACGCTGCTGCGGCATTATAAGCACANCTTTCGGAGCCTGTTCGTTCAC 419
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
5083R-2.in silico 361 GGTATTACTTCGACGCTGCTGCGGCATTATAAGCACATCTTTCGGAGCCTGTTCGTTCAC 419
5083R-2.IR_full 421 CCCGGCAAGGGTGNGGACCCGGGTGCCGCGAATCACTACCAAGCGCTGTATGAGTTCGGT 479
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
5083R-2.in silico 421 CCCGGCAAGGGTGCGGACCCGGGTGCCGCGAATCACTACCAAGCGCTGTATGAGTTCGGT 479
5083R-2.IR_full 481 TGGTTGCTCTTCCTGGTCATTCG 502
|||||||||||||||||||||||
5083R-2.in silico 481 TGGTTGCTCTTCCTGGTCATTCG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079648.2 |
CG5083-RA (Rbf2), mRNA |
| 0.2 |
1 |
4 |
3 |
2 |
NM_143582.1 |
CG15552-RA (Sox100B), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168539.1 |
CG32119-RA (CG32119), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_142145.2 |
CG7265-RA (CG7265), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_141848.1 |
CG6908-RA (CG6908), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142827.1 |
CG6972-RA (CG6972), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170036.1 |
CG31239-RA (CG31239), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132525.1 |
CG15740-RA (CG15740), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_175968.1 |
CG12787-RF, transcript variant F (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_175966.1 |
CG12787-RD, transcript variant D (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_175967.1 |
CG12787-RE, transcript variant E (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_164603.1 |
CG12787-RB, transcript variant B (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135032.1 |
CG12787-RC, transcript variant C (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135031.3 |
CG12787-RA, transcript variant A (hoe1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141141.1 |
CG14459-RA (CG14459), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_141702.2 |
CG12806-RA (Teh1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078570.2 |
CG1763-RA (nod), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137166.3 |
CG7761-RA (pcs), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078609.2 |
CG11654-RA (Ahcy13), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043247.1 |
CG6535-RB (tefu), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168420.1 |
CG32067-RA, transcript variant A (simj), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140150.2 |
CG32067-RC, transcript variant C (simj), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168421.1 |
CG32067-RB, transcript variant B (simj), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132393.2 |
CG2221-RA (l(1)G0289), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141115.1 |
CG14562-RA (CG14562), mRNA |
| 0 |
0 |
1 |
1 |
1 |
NM_140184.2 |
CG7628-RA (CG7628), mRNA |
| 0 |
0 |
0 |
7 |
11 |
NM_001014624.1 |
CG33555-RC, transcript variant C (btsz), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_078793.4 |
CG9556-RB, transcript variant B (alien), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_136372.2 |
CG3274-RA (Bap170), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_164843.1 |
CG9556-RA, transcript variant A (alien), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| +- |
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| DB |
 |
| +- |
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| DC |
 |
| +- |
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| DM |
 |
| +- |
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| VM |
 |
| +- |
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| VB |
 |
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