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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5071R-1 |
| Symbol |
CG5071 |
Full Name |
CG5071 |
| CG No |
CG5071 |
Old CG No |
CG5071 |
| Synonyms |
BcDNA:AT31683, CG5071 |
| Accession No (Link to NCBI) |
NM_170232.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
late pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGAGTCCATCCAGGTGCCAA |
| Primer Seq. 3' |
aatctagaggtaccGTCCTGGTGCAATGGGACATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGAGTCCAT CCAGGTGCCA AAGGAGCTGA AATCGCTGCT GAGCTGCGGT CTATGCCATC
0061 GCCCGTACGA TCTGGCCACC GGCNTTCTGC CCCAGGAGCT CGCCTGCCGG CACAGCTTCT
0121 GCAAGAAGTG CGTGCAAAGA AACACCGACC ACAACTCGTC CGAGTGTATT TGCAATTTGT
0181 GCGGATATCG CAcGCAGCTG GATGGCCAGC AGCTACCGCA ATCCGTAACC ATCATGTATC
0241 TGCTCCGTGA GCTGCCAGCT TTGATTCTGG GCAGAGCCAT GTTGGATTTT TCGTACAAAA
0301 GGAGCAGCAC TATTGAAATG TCTATTGAAG AAGCACCCAG CAGCCCATCG AAAAACTGGG
0361 TGGAAGACAT CAACGTGGAG AGCTTTCTGG CCACCAGTGC GGAGCACTGT TTCATCCATG
0421 CCATGCCGAA CTCCACGTGG TGTCACAATT GCCAACGTCT GCTGTGCCGT GCCTGCTCGG
0481 ATGTCCCATT GCACCAGGAC |
| in silico PCR Fragment |
0001 TGGAGTCCAT CCAGGTGCCA AAGGAGCTGA AATCGCTGCT GAGCTGCGGT CTATGCCATC
0061 GCCCGTACGA TCTGGCCACC GGCCTTCTGC CCCAGGAGCT CGCCTGCCGG CACAGCTTCT
0121 GCAAGAAGTG CGTGCAAAGA AACACCGACC ACAACTCGTC CGAGTGTATT TGCAATTTGT
0181 GCGGATATCG CACGCAGCTG GATGGCCAGC AGCTACCGCA ATCCGTAACC ATCATGTATC
0241 TGCTCCGTGA GCTGCCAGCT TTGATTCTGG GCAGAGCCAT GTTGGATTTT TCGTACAAAA
0301 GGAGCAGCAC TATTGAAATG TCTATTGAAG AAGCACCCAG CAGCCCATCG AAAAACTGGG
0361 TGGAAGACAT CAACGTGGAG AGCTTTCTGG CCACCAGTGC GGAGCACTGT TTCATCCATG
0421 CCATGCCGAA CTCCACGTGG TGTCACAATT GCCAACGTCT GCTGTGCCGT GCCTGCTCGG
0481 ATGTCCCATT GCACCAGGAC |
| Assemble Data |
5071R-1.IR_full 1 TGGAGTCCATCCAGGTGCCAAAGGAGCTGAAATCGCTGCTGAGCTGCGGTCTATGCCATC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 1 TGGAGTCCATCCAGGTGCCAAAGGAGCTGAAATCGCTGCTGAGCTGCGGTCTATGCCATC 60
5071R-1.IR_full 61 GCCCGTACGATCTGGCCACCGGCNTTCTGCCCCAGGAGCTCGCCTGCCGGCACAGCTTCT 120
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
5071R-1.in silico 61 GCCCGTACGATCTGGCCACCGGCCTTCTGCCCCAGGAGCTCGCCTGCCGGCACAGCTTCT 120
5071R-1.IR_full 121 GCAAGAAGTGCGTGCAAAGAAACACCGACCACAACTCGTCCGAGTGTATTTGCAATTTGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 121 GCAAGAAGTGCGTGCAAAGAAACACCGACCACAACTCGTCCGAGTGTATTTGCAATTTGT 180
5071R-1.IR_full 181 GCGGATATCGCACGCAGCTGGATGGCCAGCAGCTACCGCAATCCGTAACCATCATGTATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 181 GCGGATATCGCACGCAGCTGGATGGCCAGCAGCTACCGCAATCCGTAACCATCATGTATC 240
5071R-1.IR_full 241 TGCTCCGTGAGCTGCCAGCTTTGATTCTGGGCAGAGCCATGTTGGATTTTTCGTACAAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 241 TGCTCCGTGAGCTGCCAGCTTTGATTCTGGGCAGAGCCATGTTGGATTTTTCGTACAAAA 300
5071R-1.IR_full 301 GGAGCAGCACTATTGAAATGTCTATTGAAGAAGCACCCAGCAGCCCATCGAAAAACTGGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 301 GGAGCAGCACTATTGAAATGTCTATTGAAGAAGCACCCAGCAGCCCATCGAAAAACTGGG 360
5071R-1.IR_full 361 TGGAAGACATCAACGTGGAGAGCTTTCTGGCCACCAGTGCGGAGCACTGTTTCATCCATG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 361 TGGAAGACATCAACGTGGAGAGCTTTCTGGCCACCAGTGCGGAGCACTGTTTCATCCATG 420
5071R-1.IR_full 421 CCATGCCGAACTCCACGTGGTGTCACAATTGCCAACGTCTGCTGTGCCGTGCCTGCTCGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5071R-1.in silico 421 CCATGCCGAACTCCACGTGGTGTCACAATTGCCAACGTCTGCTGTGCCGTGCCTGCTCGG 480
5071R-1.IR_full 481 ATGTCCCATTGCACCAGGAC 500
||||||||||||||||||||
5071R-1.in silico 481 ATGTCCCATTGCACCAGGAC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_170232.1 |
CG5071-RA, transcript variant A (CG5071), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_143149.1 |
CG5071-RB, transcript variant B (CG5071), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_142747.1 |
CG13409-RA (CG13409), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140083.1 |
CG18179-RA (CG18179), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_079032.2 |
CG18250-RA, transcript variant A (Dg), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143468.1 |
CG12068-RA (CG12068), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079036.3 |
CG7773-RA (fidipidine), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080071.2 |
CG12028-RA (dib), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_135335.2 |
CG7424-RA (RpL36A), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_132885.2 |
CG3679-RA (CG3679), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_136444.2 |
CG12736-RA (CG12736), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141688.1 |
CG8484-RA (topi), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169857.1 |
CG31244-RA (CG31244), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_206387.1 |
CG32171-RH, transcript variant H (Lmpt), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170635.1 |
CG32171-RD, transcript variant D (Lmpt), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168924.2 |
CG32438-RB, transcript variant B (Smc5), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141077.3 |
CG32438-RA, transcript variant A (Smc5), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206419.1 |
CG32438-RD, transcript variant D (Smc5), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_206438.1 |
CG17603-RB, transcript variant B (Taf1), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_206437.1 |
CG17603-RC, transcript variant C (Taf1), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_057608.4 |
CG17603-RA, transcript variant A (Taf1), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167408.1 |
CG32600-RA (dpr8), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165173.2 |
CG17161-RD, transcript variant D (grp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165172.2 |
CG17161-RC, transcript variant C (grp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165171.2 |
CG17161-RB, transcript variant B (grp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057663.3 |
CG17161-RA, transcript variant A (grp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_138984.2 |
CG5712-RA (ACXD), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_139402.2 |
CG12018-RA (CG12018), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_140134.1 |
CG6559-RA (CG6559), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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