NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5064R-3

Symbol Srp68 Full Name Srp68

CG No CG5064 Old CG No CG5064

Synonyms CG5064, Srp68

Accession No (Link to NCBI) NM_140016.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGCATCCGTCGATTGAGGAA

Primer Seq. 3'

aatctagaggtaccTGATGTTGTAGGCGCAGTAGC

Predicted Fragment Size

IR fragment full Seq

0001 CCGCAGTCCG TCGATTGAGG AAGGCCCTGA AGTACCCCCA GGGCGACAAG CGGCACTTCA 
0061 AAAGACGCGA CGTGACCATT GGCCAATTGA CAGGCAAGAA GGCGGACGAG CGTTTCATCC 
0121 ACATCCCATT GATCTGCGCC GAGCGGGCCT GGGCGTATGC CATGCAGCTC AAGCAGGAGT 
0181 CCAATACGGA GCCGCGAAAG CGTTTCCATC TGGTCAAACA AGCTGCGCAG GGCCTGTTTC 
0241 TATGCCCTCC AGCTCCAGGA GTTGTGTAAT ACGGAGGCTT TCGATGCGCG CACTAAGCTA 
0301 GAGTGCGAAG CCTATGTTGC CTGGATGCAC GGAACCCTGC ACTTTGAGCT GCAGCTATGG 
0361 AAAACCGCTG GCGAGCACCT AAAGCGCGCC CAAGTGGTGT ACGAAAACCT GGGCAAGGCT 
0421 CTGCCCGAAG ATGAACAAGA GCTTTACCGC GCTAAGGTTA ATGAATTTAC GCCCAATCTG 
0481 CGCTACTGCG CCTACAACAT CA

in silico PCR Fragment

0001 CCGCA-TCCG TCGATTGAGG AAGGCCCTGA AGTACCCCCA GGGCGACAAG CGGCACTTCA 
0061 AAAGACGCGA CGTGACCATT GGCCAATTGA CAGGCAAGAA GGCGGACGAG CGTTTCATCC 
0121 ACATCCCATT GATCTGCGCC GAGCGGGCCT GGGCGTATGC CATGCAGCTC AAGCAGGAGT 
0181 CCAATACGGA GCCGCGAAAG CGTTTCCATC TGGTC-AACA AGCTGCGCAG GGCCTGTTTC 
0241 TATGCCCTCC AGCTCCAGGA GTTGTGTAAT ACGGAGGCTT TCGATGCGCG CACTAAGCTA 
0301 GAGTGCGAAG CCTATGTTGC CTGGATGCAC GGAACCCTGC ACTTTGAGCT GCAGCTATGG 
0361 AAAACCGCTG GCGAGCACCT AAAGCGCGCC CAAGTGGTGT ACGAAAACCT GGGCAAGGCT 
0421 CTGCCCGAAG ATGAACAAGA GCTTTACCGC GCTAAGGTTA ATGAATTTAC GCCCAATCTG 
0481 CGCTACTGCG CCTACAACAT CA

Assemble Data


5064R-3.IR_full       1   CCGCAGTCCGTCGATTGAGGAAGGCCCTGAAGTACCCCCAGGGCGACAAGCGGCACTTCA 60
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     1   CCGCA-TCCGTCGATTGAGGAAGGCCCTGAAGTACCCCCAGGGCGACAAGCGGCACTTCA 60


5064R-3.IR_full       61  AAAGACGCGACGTGACCATTGGCCAATTGACAGGCAAGAAGGCGGACGAGCGTTTCATCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     61  AAAGACGCGACGTGACCATTGGCCAATTGACAGGCAAGAAGGCGGACGAGCGTTTCATCC 120


5064R-3.IR_full       121 ACATCCCATTGATCTGCGCCGAGCGGGCCTGGGCGTATGCCATGCAGCTCAAGCAGGAGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     121 ACATCCCATTGATCTGCGCCGAGCGGGCCTGGGCGTATGCCATGCAGCTCAAGCAGGAGT 180


5064R-3.IR_full       181 CCAATACGGAGCCGCGAAAGCGTTTCCATCTGGTCAAACAAGCTGCGCAGGGCCTGTTTC 240
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
5064R-3.in silico     181 CCAATACGGAGCCGCGAAAGCGTTTCCATCTGGTC-AACAAGCTGCGCAGGGCCTGTTTC 240


5064R-3.IR_full       241 TATGCCCTCCAGCTCCAGGAGTTGTGTAATACGGAGGCTTTCGATGCGCGCACTAAGCTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     241 TATGCCCTCCAGCTCCAGGAGTTGTGTAATACGGAGGCTTTCGATGCGCGCACTAAGCTA 300


5064R-3.IR_full       301 GAGTGCGAAGCCTATGTTGCCTGGATGCACGGAACCCTGCACTTTGAGCTGCAGCTATGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     301 GAGTGCGAAGCCTATGTTGCCTGGATGCACGGAACCCTGCACTTTGAGCTGCAGCTATGG 360


5064R-3.IR_full       361 AAAACCGCTGGCGAGCACCTAAAGCGCGCCCAAGTGGTGTACGAAAACCTGGGCAAGGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     361 AAAACCGCTGGCGAGCACCTAAAGCGCGCCCAAGTGGTGTACGAAAACCTGGGCAAGGCT 420


5064R-3.IR_full       421 CTGCCCGAAGATGAACAAGAGCTTTACCGCGCTAAGGTTAATGAATTTACGCCCAATCTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5064R-3.in silico     421 CTGCCCGAAGATGAACAAGAGCTTTACCGCGCTAAGGTTAATGAATTTACGCCCAATCTG 480


5064R-3.IR_full       481 CGCTACTGCGCCTACAACATCA 502
                          ||||||||||||||||||||||
5064R-3.in silico     481 CGCTACTGCGCCTACAACATCA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140016.2	CG5064-RA (Srp68), mRNA
0.2	1	2	2	6	NM_143031.1	CG11375-RA (polybromo), mRNA
0	0	2	3	6	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	1	5	5	NM_079598.2	CG6913-RA (Fer3), mRNA
0	0	1	3	8	NM_164857.1	CG3811-RA, transcript variant A (Oatp30B), mRNA
0	0	1	3	8	NM_205946.1	CG3811-RC, transcript variant C (Oatp30B), mRNA
0	0	1	3	8	NM_135449.2	CG3811-RB, transcript variant B (Oatp30B), mRNA
0	0	1	3	8	NM_205945.1	CG3811-RD, transcript variant D (Oatp30B), mRNA
0	0	1	2	5	NM_143605.2	CG11339-RA (CG11339), mRNA
0	0	1	2	3	NM_136001.1	CG5131-RA (CG5131), mRNA
0	0	1	2	1	NM_206089.1	CG33473-RB (luna), mRNA
0	0	0	7	2	NM_142971.2	CG5524-RA (CG5524), mRNA
0	0	0	6	14	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	6	6	NM_001042819.1	CG5424-RF, transcript variant F (f), mRNA
0	0	0	6	5	NM_078660.2	CG5424-RB, transcript variant B (f), mRNA
0	0	0	6	5	NM_176745.1	CG5424-RD, transcript variant D (f), mRNA
0	0	0	6	5	NM_176746.1	CG5424-RC, transcript variant C (f), mRNA
0	0	0	6	5	NM_167563.2	CG5424-RA, transcript variant A (f), mRNA
0	0	0	6	4	NM_136173.1	CG13966-RA (CG13966), mRNA
0	0	0	6	3	NM_079644.2	CG5192-RB (Rh6), mRNA
0	0	0	6	3	NM_134725.1	CG4552-RA (CG4552), mRNA
0	0	0	5	9	NM_136647.2	CG8809-RA (Camta), mRNA
0	0	0	5	7	NM_134714.3	CG4341-RA (CG4341), mRNA
0	0	0	5	5	NM_078645.2	CG4252-RA (mei-41), mRNA
0	0	0	4	8	NM_132087.3	CG15893-RA (CG15893), mRNA
0	0	0	4	6	NM_132969.2	CG8918-RA (CG8918), mRNA
0	0	0	4	5	NM_166183.1	CG8332-RB, transcript variant B (RpS15), mRNA
0	0	0	4	5	NM_136055.2	CG10376-RA (CG10376), mRNA
0	0	0	4	5	NM_206345.1	CG32103-RA, transcript variant A (CG32103), mRNA
0	0	0	4	5	NM_137292.2	CG8332-RA, transcript variant A (RpS15), mRNA

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