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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5034R-2 |
| Symbol |
GATAd |
Full Name |
GATAd |
| CG No |
CG5034 |
Old CG No |
CG5034 |
| Synonyms |
dGATAd, dGATA-D, CG5034, GATAd |
| Accession No (Link to NCBI) |
NM_135539.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Buchon N, Osman D, David FP, Fang HY, Boquete JP, Deplancke B, Lemaitre B.
Morphological and molecular characterization of adult midgut compartmentalization in Drosophila.
Cell Rep (2013) 3(5) 1725-38
[
PubMed ID = 23643535
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTTAACGAGTGCGATGTTG |
| Primer Seq. 3' |
aatctagaggtaccTGTTTGGGATTGGGATAGCGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTTAACGA GTGCGATGTT GCAGTACTAC AGATTTACGA TAATTCGTCG CATAATAATA
0061 GTGCCCCAGA CAGAATATCG GCCGATTTTC GTGGCGTCNC GAATCGTGCC AACTGCTTTT
0121 GCAGCGCTTT AAGCCCCAAT CCGTGCAGTT GCATCGTTGA TAACCCGCTA TCAGCCAACA
0181 AAAATGAACG GCGCAACCAG GGAACACCCG TACCCGTGCC CATACCCATA CCCGTTTCCG
0241 TGCCTGTGCC CGTTCCAAGC CAGCAGATCC AATCGCAAAC CCTTCACCAT CACCAAAACC
0301 AAACNTAAAA TCCATTACAC GCCAAGTGTG CCTTCTGCTG CGGACGCAAA GTCGAGTGAG
0361 CGCGAACGCC AATGCCAATT GGAGTCGGAC ATCGCCGAGA GATCAACAGT ATTCAAAAGT
0421 CAGTCATCGT CGGGATCACG AACANATAGA ATATAGCGGG GAGACGCCAT CCCCCTCCgN
0481 ATCGCTATCC CAATCCCAAA CA |
| in silico PCR Fragment |
0001 TGGTTAACGA GTGCGATGTT GCAGTACTAC AGATTTACGA TAATTCGTCG CATAATAATA
0061 GTGCCCCAGA CAGAATATCG GCCGATTTTC GTGGCGTCGC GAATCGTGCC AACTGCTTTT
0121 GCAGCGCTTT AAGCCCCAAT CCGTGCAGTT GCATCGTTGA TAACCCGCTA TCAGCCAACA
0181 AAAATGAACG GCGCAACCAG GGAACACCCG TACCCGTGCC CATACCCATA CCCGTTTCCG
0241 TGCCTGTGCC CGTTCCAAGC CAGCAGATCC AATCGCAAAC CCTTCACCAT CACCAAAACC
0301 AAAC-TAAAA TCCATTACAC GCCAAGTGTG CCTTCTGCTG CGGACGCAAA GTCGAGTGAG
0361 CGCGAACGCC AATGCCAATT GGAGTCGGAC ATCGCCGAGA GATCAACAGT ATTCAAAAGT
0421 CAGTCATCGT C-GGATCACG AACATATAGA ATATAGCGGG GAGACGCCAT CCCCCTCCGC
0481 ATCGCTATCC CAATCCCAAA CA |
| Assemble Data |
5034R-2.IR_full 1 TGGTTAACGAGTGCGATGTTGCAGTACTACAGATTTACGATAATTCGTCGCATAATAATA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 1 TGGTTAACGAGTGCGATGTTGCAGTACTACAGATTTACGATAATTCGTCGCATAATAATA 60
5034R-2.IR_full 61 GTGCCCCAGACAGAATATCGGCCGATTTTCGTGGCGTCNCGAATCGTGCCAACTGCTTTT 120
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
5034R-2.in silico 61 GTGCCCCAGACAGAATATCGGCCGATTTTCGTGGCGTCGCGAATCGTGCCAACTGCTTTT 120
5034R-2.IR_full 121 GCAGCGCTTTAAGCCCCAATCCGTGCAGTTGCATCGTTGATAACCCGCTATCAGCCAACA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 121 GCAGCGCTTTAAGCCCCAATCCGTGCAGTTGCATCGTTGATAACCCGCTATCAGCCAACA 180
5034R-2.IR_full 181 AAAATGAACGGCGCAACCAGGGAACACCCGTACCCGTGCCCATACCCATACCCGTTTCCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 181 AAAATGAACGGCGCAACCAGGGAACACCCGTACCCGTGCCCATACCCATACCCGTTTCCG 240
5034R-2.IR_full 241 TGCCTGTGCCCGTTCCAAGCCAGCAGATCCAATCGCAAACCCTTCACCATCACCAAAACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 241 TGCCTGTGCCCGTTCCAAGCCAGCAGATCCAATCGCAAACCCTTCACCATCACCAAAACC 300
5034R-2.IR_full 301 AAACNTAAAATCCATTACACGCCAAGTGTGCCTTCTGCTGCGGACGCAAAGTCGAGTGAG 360
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 301 AAAC-TAAAATCCATTACACGCCAAGTGTGCCTTCTGCTGCGGACGCAAAGTCGAGTGAG 360
5034R-2.IR_full 361 CGCGAACGCCAATGCCAATTGGAGTCGGACATCGCCGAGAGATCAACAGTATTCAAAAGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5034R-2.in silico 361 CGCGAACGCCAATGCCAATTGGAGTCGGACATCGCCGAGAGATCAACAGTATTCAAAAGT 420
5034R-2.IR_full 421 CAGTCATCGTCGGGATCACGAACANATAGAATATAGCGGGGAGACGCCATCCCCCTCCGN 480
||||||||||| |||||||||||| ||||||||||||||||||||||||||||||||||
5034R-2.in silico 421 CAGTCATCGTC-GGATCACGAACATATAGAATATAGCGGGGAGACGCCATCCCCCTCCGC 480
5034R-2.IR_full 481 ATCGCTATCCCAATCCCAAACA 502
||||||||||||||||||||||
5034R-2.in silico 481 ATCGCTATCCCAATCCCAAACA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
10 |
NM_135539.2 |
CG5034-RA (GATAd), mRNA |
| 0.41 |
2 |
6 |
3 |
14 |
NM_136859.2 |
CG8991-RA (Sobp), mRNA |
| 0 |
0 |
5 |
4 |
12 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 0 |
0 |
5 |
4 |
12 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 0 |
0 |
5 |
4 |
12 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 0 |
0 |
5 |
4 |
12 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 0 |
0 |
5 |
4 |
11 |
NM_001014632.1 |
CG31243-RG, transcript variant G (cpo), mRNA |
| 0 |
0 |
5 |
4 |
11 |
NM_001014631.1 |
CG31243-RH, transcript variant H (cpo), mRNA |
| 0 |
0 |
3 |
14 |
26 |
NM_169851.1 |
CG31475-RA (CG31475), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078493.2 |
CG4206-RA (Mcm3), mRNA |
| 0 |
0 |
2 |
7 |
8 |
NM_140917.1 |
CG14186-RA (CG14186), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_078960.4 |
CG2204-RA, transcript variant A (G-oalpha47A), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_206079.1 |
CG2204-RI, transcript variant I (G-oalpha47A), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_134843.2 |
CG3515-RA (CG3515), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_132683.1 |
CG12177-RA (CG12177), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_164509.2 |
CG2991-RA, transcript variant A (CG2991), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_134881.5 |
CG2991-RB, transcript variant B (CG2991), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_057790.4 |
CG32848-RA (VAChT), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168324.2 |
CG4432-RA, transcript variant A (PGRP-LC), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001015396.1 |
CG40411-PC.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001015395.1 |
CG40411-PE.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001015397.1 |
CG40411-PD.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141593.2 |
CG11968-RA (CG11968), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164510.2 |
CG2991-RC, transcript variant C (CG2991), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139460.1 |
CG9004-RA (CG9004), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079152.3 |
CG1007-RA (emc), mRNA |
| 0 |
0 |
0 |
15 |
13 |
NM_164887.1 |
CG31714-RA (CG31714), mRNA |
| 0 |
0 |
0 |
8 |
9 |
NM_132420.2 |
CG32676-RA (CG32676), mRNA |
| 0 |
0 |
0 |
7 |
36 |
NM_140298.2 |
CG6906-RA (CAH2), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_166075.1 |
CG30475-RA (CG30475), mRNA |
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