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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5005R-3 
 Symbol HLH54F  Full Name HLH54F 
 CG No CG5005  Old CG No CG5005 
 Synonyms bHLH54F, HLH102C, CG5005, HLH54F 
 Accession No (Link to NCBI) NM_057954.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGCCACGCAAGAAGCGCAGTT 
 Primer Seq. 3' 
aatctagaggtaccGGCGGATGAGGTGGTTGATGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGCCACGCAA GAAGCGCAGT TCCAGCCCCA CGGAgTTCTT TGATGACGAC TTCGATGAGG 
0061 ATGCCAGTTC TCAAAGCTCC CAACCGCCGG TGCAGCGAAA TGCGGCAAAT GCCCGGGAGC 
0121 GGATGCGTAT GCGGGTTTTA TCCAGCGCCT ACGGACGTTT GAAGACCAAG CTGCCCAACA 
0181 TTCCGCCGGA CACGAAGCTC TCCAAGTTGG ACACGTTGCG TTTGGCCACG CTCTACATCA 
0241 AGCAGCTAAT TACGGCTGTG GAGACAGGCA GCCACTCGCA GAACCACCCG CACAACCACA 
0301 ACCAGCACCA CAGCCTCAAC CACAGTCACT CGAGTACCAC CAGTTCCGAA GGCCTGGACA 
0361 CAAGTCACAT GGCAGATTCG TCCGGTGGAG GCAATTATAA TTTCCACAAC AACGGACACG 
0421 GCATGAGCTG GCCCTTTGAG TTCCACCAGA GTAGCCGGAG TTAGGCATTT GCCCCATCAT 
0481 CATCAACCAC CTCATCCGCC  
 in silico PCR Fragment 
0001 TGCCACGCAA GAAGCGCAGT TCCAGCCCCA CGGAGTTCTT TGATGACGAC TTCGATGAGG 
0061 ATGCCAGTTC TCAAAGCTCC CAACCGCCGG TGCAGCGAAA TGCGGCAAAT GCCCGGGAGC 
0121 GGATGCGTAT GCGGGTTTTA TCCAGCGCCT ACGGACGTTT GAAGACCAAG CTGCCCAACA 
0181 TTCCGCCGGA CACGAAGCTC TCCAAGTTGG ACACGTTGCG TTTGGCCACG CTCTACATCA 
0241 AGCAGCTAAT TACGGCTGTG GAGACAGGCA GCCACTCGCA GAACCACCCG CACAACCACA 
0301 ACCAGCACCA CAGCCTCAAC CACAGTCACT CGAGTACCAC CAGTTCCGAA GGCCTGGACA 
0361 CAAGTCACAT GGCAGATTCG TCCGGTGGAG GCAATTATAA TTTCCACAAC AACGGACACG 
0421 GCATGAGCTG GCCCTTTGAG TTCCACCAGA GTAGCCGGAG TTTGGCATTT GCCCCATCAT 
0481 CATCAACCAC CTCATCCGCC  
 Assemble Data 

5005R-3.IR_full       1   TGCCACGCAAGAAGCGCAGTTCCAGCCCCACGGAGTTCTTTGATGACGACTTCGATGAGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     1   TGCCACGCAAGAAGCGCAGTTCCAGCCCCACGGAGTTCTTTGATGACGACTTCGATGAGG 60


5005R-3.IR_full       61  ATGCCAGTTCTCAAAGCTCCCAACCGCCGGTGCAGCGAAATGCGGCAAATGCCCGGGAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     61  ATGCCAGTTCTCAAAGCTCCCAACCGCCGGTGCAGCGAAATGCGGCAAATGCCCGGGAGC 120


5005R-3.IR_full       121 GGATGCGTATGCGGGTTTTATCCAGCGCCTACGGACGTTTGAAGACCAAGCTGCCCAACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     121 GGATGCGTATGCGGGTTTTATCCAGCGCCTACGGACGTTTGAAGACCAAGCTGCCCAACA 180


5005R-3.IR_full       181 TTCCGCCGGACACGAAGCTCTCCAAGTTGGACACGTTGCGTTTGGCCACGCTCTACATCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     181 TTCCGCCGGACACGAAGCTCTCCAAGTTGGACACGTTGCGTTTGGCCACGCTCTACATCA 240


5005R-3.IR_full       241 AGCAGCTAATTACGGCTGTGGAGACAGGCAGCCACTCGCAGAACCACCCGCACAACCACA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     241 AGCAGCTAATTACGGCTGTGGAGACAGGCAGCCACTCGCAGAACCACCCGCACAACCACA 300


5005R-3.IR_full       301 ACCAGCACCACAGCCTCAACCACAGTCACTCGAGTACCACCAGTTCCGAAGGCCTGGACA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     301 ACCAGCACCACAGCCTCAACCACAGTCACTCGAGTACCACCAGTTCCGAAGGCCTGGACA 360


5005R-3.IR_full       361 CAAGTCACATGGCAGATTCGTCCGGTGGAGGCAATTATAATTTCCACAACAACGGACACG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5005R-3.in silico     361 CAAGTCACATGGCAGATTCGTCCGGTGGAGGCAATTATAATTTCCACAACAACGGACACG 420


5005R-3.IR_full       421 GCATGAGCTGGCCCTTTGAGTTCCACCAGAGTAGCCGGAGTTAGGCATTTGCCCCATCAT 480
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
5005R-3.in silico     421 GCATGAGCTGGCCCTTTGAGTTCCACCAGAGTAGCCGGAGTTTGGCATTTGCCCCATCAT 480


5005R-3.IR_full       481 CATCAACCACCTCATCCGCC 500
                          ||||||||||||||||||||
5005R-3.in silico     481 CATCAACCACCTCATCCGCC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_057954.2  CG5005-RA (HLH54F), mRNA 
0   0  26  28  138  NM_170227.2  CG31439-RA (CG31439), mRNA 
0   0  6  6  12  NM_132667.1  CG15756-RA (CG15756), mRNA 
0   0  4  3  2  NM_136255.1  CG8679-RA (CG8679), mRNA 
0   0  3  5  10  NM_080055.2  CG3895-RA (ph-d), mRNA 
0   0  3  5  5  NM_057523.3  CG18412-RA (ph-p), mRNA 
0   0  3  4  8  NM_168627.1  CG7656-RC, transcript variant C (CG7656), mRNA 
0   0  3  4  8  NM_140526.3  CG7656-RB, transcript variant B (CG7656), mRNA 
0   0  3  4  8  NM_168628.1  CG7656-RA, transcript variant A (CG7656), mRNA 
0   0  3  4  3  NM_170170.1  CG31118-RA (RabX4), mRNA 
0   0  3  3  14  NM_135589.2  CG17104-RA (CG17104), mRNA 
0   0  3  3  2  NM_142265.2  CG4979-RA (sxe2), mRNA 
0   0  2  3  8  NM_176458.2  CG4509-RB (CG4509), mRNA 
0   0  2  3  3  NM_137779.1  CG13490-RA (CG13490), mRNA 
0   0  2  3  2  NM_057770.3  CG3193-RA (crn), mRNA 
0   0  2  2  15  NM_142330.1  CG5225-RA (CG5225), mRNA 
0   0  2  2  7  NM_143780.2  CG10130-RA (Sec61beta), mRNA 
0   0  2  2  4  NM_132066.1  CG4320-RA (raptor), mRNA 
0   0  2  2  3  NM_140011.2  CG5660-RA (CG5660), mRNA 
0   0  2  2  2  NM_079512.2  CG2899-RA (ksr), mRNA 
0   0  2  2  2  NM_169682.1  CG31291-RB, transcript variant B (CG31291), mRNA 
0   0  2  2  2  NM_169083.1  CG31549-RA (CG31549), mRNA 
0   0  1  7  10  NM_143061.1  CG13648-RA (tnc), mRNA 
0   0  1  6  7  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  1  4  4  NM_169137.1  CG10295-RC, transcript variant C (Pak), mRNA 
0   0  1  4  4  NM_169136.1  CG10295-RA, transcript variant A (Pak), mRNA 
0   0  1  4  4  NM_079942.3  CG10295-RB, transcript variant B (Pak), mRNA 
0   0  1  3  11  NM_170168.1  CG6863-RB, transcript variant B (tok), mRNA 
0   0  1  3  11  NM_057531.3  CG6863-RA, transcript variant A (tok), mRNA 
0   0  1  3  7  NM_169205.1  CG2747-RB, transcript variant B (CG2747), mRNA 

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