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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4952R-2 
 Symbol dac  Full Name dachshund 
 CG No CG4952  Old CG No CG4952 
 Synonyms CG4952, DAC, Dac, Dm-DAC, dach, BG:DS02780.3, l(2)36Ae, spn, l(2)rK364, dac 
 Accession No (Link to NCBI) NM_165160.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Eade KT, Fancher HA, Ridyard MS, Allan DW.
Developmental transcriptional networks are required to maintain neuronal subtype identity in the mature nervous system.
PLoS Genet (2012) 8(2) e1002501 [ PubMed ID = 22383890 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACAGCAGCAGCACAATGGACG 
 Primer Seq. 3' 
aatctagaggtaccCGACCTTCTGACCCCGGTACT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACAGcAGCAG CACAATGGAC NCGNNGNTAG CAACAGTTCG CGTGGAGcCA ACGAGGATCG 
0061 TCCAAATGGA CGGAGCTCGC ACACAGGACG AGGATCCAGT TGCTCACCGG CCAGTTCACC 
0121 AAGTCGCCAT CCCAGTGCCG TCAGTCCGGT GAGCNCCCTC AACCACTCCA TGATGCAGCA 
0181 GATGCAGCAA CAGCAGCAAC AACAGCAGCA GCAACAGCAG CAGCAGCAAN ACCATCATGC 
0241 TCAGCCCCCC GCCACATGGA ATGCCATCGG GCAACGGACT GCCGACGGGC CTACCGCCGA 
0301 GAATGCCCCA TGGACTTGCC GCCCCATTCG CTGGGGCTCC TCAACTCCCT GCAGATGATG 
0361 CACCATGCCT CCCCCTTGGA ACTGATGGCC GCCGCCCATC ACCATGTGCC GCCAAGGTCG 
0421 TACAACTCAC CGCCGCCGAT CTCCACCTCG GATCCCAGTG CCAATGAGTG CAAGCTGGTG 
0481 GAGTACCGGG GTCAGAAGGT CG 
 in silico PCR Fragment 
0001 ACAGCAGCAG CACAATGGAC GCGACGGTAG CAACAGTTCG CGTGGAGCCA ACGAGGATCG 
0061 TCCAAATGGA CGGAGCTCGC ACACAGGACG AGGATCCAGT TGCTCACCGG CCAGTTCACC 
0121 AAGTCGCCAT CCCAGTGCCG TCAGTCCGGT GAGCTCCCTC AACCACTCCA TGATGCAGCA 
0181 GATGCAGCAA CAGCAGCAAC AACAGCAGCA GCAACAGCAG CAGCAGCAAC ACCATCA-GC 
0241 TCAGCCCCCC GCCACATGGA ATGCCATCGG GCAACGGACT GCCGACGGGC CTACCGCCCA 
0301 GAATGCCCCA TGGAC-TGCC GCCCCATTCG CTGGGGCTCC TCAACTCCCT GCAGATGATG 
0361 CACCATGCCT CCCCCTTGGA ACTGATGGCC GCCGCCCATC ACCATGTGCC GCCAAGGTCG 
0421 TACAACTCAC CGCCGCCGAT CTCCACCTCG GATCCCAGTG CCAATGAGTG CAAGCTGGTG 
0481 GAGTACCGGG GTCAGAAGGT CG 
 Assemble Data 

4952R-2.IR_full       1   ACAGCAGCAGCACAATGGACNCGNNGNTAGCAACAGTTCGCGTGGAGCCAACGAGGATCG 60
                          |||||||||||||||||||| ||  | |||||||||||||||||||||||||||||||||
4952R-2.in silico     1   ACAGCAGCAGCACAATGGACGCGACGGTAGCAACAGTTCGCGTGGAGCCAACGAGGATCG 60


4952R-2.IR_full       61  TCCAAATGGACGGAGCTCGCACACAGGACGAGGATCCAGTTGCTCACCGGCCAGTTCACC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4952R-2.in silico     61  TCCAAATGGACGGAGCTCGCACACAGGACGAGGATCCAGTTGCTCACCGGCCAGTTCACC 120


4952R-2.IR_full       121 AAGTCGCCATCCCAGTGCCGTCAGTCCGGTGAGCNCCCTCAACCACTCCATGATGCAGCA 180
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
4952R-2.in silico     121 AAGTCGCCATCCCAGTGCCGTCAGTCCGGTGAGCTCCCTCAACCACTCCATGATGCAGCA 180


4952R-2.IR_full       181 GATGCAGCAACAGCAGCAACAACAGCAGCAGCAACAGCAGCAGCAGCAANACCATCATGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| ||
4952R-2.in silico     181 GATGCAGCAACAGCAGCAACAACAGCAGCAGCAACAGCAGCAGCAGCAACACCATCA-GC 240


4952R-2.IR_full       241 TCAGCCCCCCGCCACATGGAATGCCATCGGGCAACGGACTGCCGACGGGCCTACCGCCGA 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
4952R-2.in silico     241 TCAGCCCCCCGCCACATGGAATGCCATCGGGCAACGGACTGCCGACGGGCCTACCGCCCA 300


4952R-2.IR_full       301 GAATGCCCCATGGACTTGCCGCCCCATTCGCTGGGGCTCCTCAACTCCCTGCAGATGATG 360
                          ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
4952R-2.in silico     301 GAATGCCCCATGGAC-TGCCGCCCCATTCGCTGGGGCTCCTCAACTCCCTGCAGATGATG 360


4952R-2.IR_full       361 CACCATGCCTCCCCCTTGGAACTGATGGCCGCCGCCCATCACCATGTGCCGCCAAGGTCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4952R-2.in silico     361 CACCATGCCTCCCCCTTGGAACTGATGGCCGCCGCCCATCACCATGTGCCGCCAAGGTCG 420


4952R-2.IR_full       421 TACAACTCACCGCCGCCGATCTCCACCTCGGATCCCAGTGCCAATGAGTGCAAGCTGGTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4952R-2.in silico     421 TACAACTCACCGCCGCCGATCTCCACCTCGGATCCCAGTGCCAATGAGTGCAAGCTGGTG 480


4952R-2.IR_full       481 GAGTACCGGGGTCAGAAGGTCG 502
                          ||||||||||||||||||||||
4952R-2.in silico     481 GAGTACCGGGGTCAGAAGGTCG 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.41  484  38  108  307  NM_165160.1  CG4952-RA, transcript variant A (dac), mRNA 
100.41  484  38  108  307  NM_165159.1  CG4952-RC, transcript variant C (dac), mRNA 
100.41  484  38  108  307  NM_001014486.1  CG4952-RG, transcript variant G (dac), mRNA 
100.41  484  38  108  307  NM_165162.1  CG4952-RB, transcript variant B (dac), mRNA 
100.41  484  38  108  307  NM_165161.1  CG4952-RD, transcript variant D (dac), mRNA 
100.41  484  38  108  307  NM_001014487.1  CG4952-RF, transcript variant F (dac), mRNA 
100.41  484  38  108  307  NM_165163.1  CG4952-RE, transcript variant E (dac), mRNA 
19.7   95  850  2170  3891  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
19.7   95  850  2170  3891  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
19.7   95  850  2170  3891  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
9.75   47  160  303  601  NM_166992.2  CG2904-RA (ec), mRNA 
9.54   46  173  488  772  NM_167000.1  CG32778-RA (CG32778), mRNA 
8.92   43  171  427  807  NM_169258.1  CG9755-RA, transcript variant A (pum), mRNA 
8.92   43  171  427  807  NM_169259.1  CG9755-RD, transcript variant D (pum), mRNA 
8.92   43  171  427  807  NM_079561.2  CG9755-RC, transcript variant C (pum), mRNA 
8.71   42  292  901  1741  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
8.71   42  292  901  1741  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
8.29   40  179  644  1131  NM_139493.2  CG2083-RA (CG2083), mRNA 
7.88   38  182  585  1142  NM_078797.2  CG13109-RA (tai), mRNA 
7.46   36  124  300  546  NM_132126.1  CG3075-RA (CG3075), mRNA 
6.43   31  224  688  1417  NM_135077.2  CG14023-RA (CG14023), mRNA 
6.43   31  193  568  1157  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
6.43   31  193  568  1157  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
6.43   31  148  408  938  NM_132403.3  CG17255-RA, transcript variant A (CG17255), mRNA 
6.43   31  148  408  938  NM_167231.3  CG17255-RB, transcript variant B (CG17255), mRNA 
6.43   31  107  194  398  NM_079119.2  CG4354-RA (slbo), mRNA 
6.22   30  164  350  459  NM_080487.2  CG10572-RA (Cdk8), mRNA 
6.22   30  111  338  629  NM_132004.2  CG4136-RA (CG4136), mRNA 
6.01   29  148  437  827  NM_176592.1  CG12071-RB, transcript variant B (CG12071), mRNA 
6.01   29  148  437  827  NM_143575.2  CG12071-RA, transcript variant A (CG12071), mRNA 

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