NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4912R-1

Symbol eEF1delta Full Name eEF1delta

CG No CG4912 Old CG No CG4912

Synonyms eEF1delta, CG4912, eEF1d

Accession No (Link to NCBI) NM_135517.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGCTACGATCTTGCCGAGAA

Primer Seq. 3'

aatctagaggtaccCCCACGGCTTAACATCCAGAA

Predicted Fragment Size

IR fragment full Seq

0001 CCGCTACGAT CTTGCCGAGA AGCGATTCTA CGAGGGTCCC CAGAAGGTAA CTGATCGCAG 
0061 CCACTACAGC CCATTAGTAA NNGAAATCGC AAAGGCTAGG GAACACATAC AGAACTCGCT 
0121 GGAAAAGATC GATGGCGTTA CTCTGGACGA TGGCTTAAAT TCCGAGCTGG CCAAGCGTCT 
0181 GGCCCAATTG GAGGGTGAAC ACAAGGAGTT GAAGACCCAG GTTTCCTTGT TGAACGAACT 
0241 TTTGACTGCC ACAGTGAAGC GCTTGGAAAC TCAGCTGAAA TTGACGAATG GAGTGTCGAA 
0301 AGAGCCCGAG GTCGAAGCGA AAAAACCAGA AGCGAACGAC GACGATGACG ATGTGGATCT 
0361 ATTTGGATCG GACAGCGAGG AGGAGGATGG AGAGGCCGCC CGCATAAGGG AGGAAAGGCT 
0421 GNGCAGCCTA TGCTGCCAAG AAGGCCAAGA AAGTTCAAAT TATTGCCAAG TCGAACATCA 
0481 TTCTGGATGT TAAGCCGTGG G

in silico PCR Fragment

0001 CCGCTACGAT CTTGCCGAGA AGCGATTCTA CGAGGGTCCC CAGAAGGTAA CTGATCGCAG 
0061 CCACTACAGC CCATTAGTAA GCGAAATCGC AAAGGCTAGG GAACACATAC AGAACTCGCT 
0121 GGAAAAGATC GATGGCGTTA CTCTGGACGA TGGCTTAAAT TCCGAGCTGG CCAAGCGTCT 
0181 GGCCCAATTG GAGGGTGAAC ACAAGGAGTT GAAGACCCAG GTTTCCTTGT TGAACGAACT 
0241 TTTGACTGCC ACAGTGAAGC GCTTGGAAAC TCAGCTGAAA TTGACGAATG GAGTGTCGAA 
0301 AGAGCCCGAG GTCGAAGCGA AAAAACCAGA AGCGAACGAC GACGATGACG ATGTGGATCT 
0361 ATTTGGATCG GACAGCGAGG AGGAGGATGG AGAGGCCGCC CGCATAAGGG AGGAAAGGCT 
0421 G-GCAGCCTA TGCTGCCAAG AAGGCCAAGA AAGTTCAAAT TATTGCCAAG TCGAACATCA 
0481 TTCTGGATGT TAAGCCGTGG G

Assemble Data


4912R-1.IR_full       1   CCGCTACGATCTTGCCGAGAAGCGATTCTACGAGGGTCCCCAGAAGGTAACTGATCGCAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     1   CCGCTACGATCTTGCCGAGAAGCGATTCTACGAGGGTCCCCAGAAGGTAACTGATCGCAG 60


4912R-1.IR_full       61  CCACTACAGCCCATTAGTAANNGAAATCGCAAAGGCTAGGGAACACATACAGAACTCGCT 120
                          ||||||||||||||||||||  ||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     61  CCACTACAGCCCATTAGTAAGCGAAATCGCAAAGGCTAGGGAACACATACAGAACTCGCT 120


4912R-1.IR_full       121 GGAAAAGATCGATGGCGTTACTCTGGACGATGGCTTAAATTCCGAGCTGGCCAAGCGTCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     121 GGAAAAGATCGATGGCGTTACTCTGGACGATGGCTTAAATTCCGAGCTGGCCAAGCGTCT 180


4912R-1.IR_full       181 GGCCCAATTGGAGGGTGAACACAAGGAGTTGAAGACCCAGGTTTCCTTGTTGAACGAACT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     181 GGCCCAATTGGAGGGTGAACACAAGGAGTTGAAGACCCAGGTTTCCTTGTTGAACGAACT 240


4912R-1.IR_full       241 TTTGACTGCCACAGTGAAGCGCTTGGAAACTCAGCTGAAATTGACGAATGGAGTGTCGAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     241 TTTGACTGCCACAGTGAAGCGCTTGGAAACTCAGCTGAAATTGACGAATGGAGTGTCGAA 300


4912R-1.IR_full       301 AGAGCCCGAGGTCGAAGCGAAAAAACCAGAAGCGAACGACGACGATGACGATGTGGATCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     301 AGAGCCCGAGGTCGAAGCGAAAAAACCAGAAGCGAACGACGACGATGACGATGTGGATCT 360


4912R-1.IR_full       361 ATTTGGATCGGACAGCGAGGAGGAGGATGGAGAGGCCGCCCGCATAAGGGAGGAAAGGCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     361 ATTTGGATCGGACAGCGAGGAGGAGGATGGAGAGGCCGCCCGCATAAGGGAGGAAAGGCT 420


4912R-1.IR_full       421 GNGCAGCCTATGCTGCCAAGAAGGCCAAGAAAGTTCAAATTATTGCCAAGTCGAACATCA 480
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4912R-1.in silico     421 G-GCAGCCTATGCTGCCAAGAAGGCCAAGAAAGTTCAAATTATTGCCAAGTCGAACATCA 480


4912R-1.IR_full       481 TTCTGGATGTTAAGCCGTGGG 501
                          |||||||||||||||||||||
4912R-1.in silico     481 TTCTGGATGTTAAGCCGTGGG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	401	0	0	2	NM_164895.1	CG4912-RA, transcript variant A (eEF1delta), mRNA
96.25	386	4	2	4	NM_135517.2	CG4912-RB, transcript variant B (eEF1delta), mRNA
0.49	2	3	2	2	NM_133036.1	CG15373-RA (CG15373), mRNA
0.49	2	2	4	10	NM_001014591.1	CG8103-RB, transcript variant B (Mi-2), mRNA
0.49	2	2	4	9	NM_140897.2	CG8103-RA, transcript variant A (Mi-2), mRNA
0.24	1	2	2	6	NM_165836.1	CG7776-RB, transcript variant B (E(Pc)), mRNA
0.24	1	2	2	6	NM_078974.2	CG7776-RA, transcript variant A (E(Pc)), mRNA
0	0	7	12	10	NM_080069.3	CG6341-RA (Ef1beta), mRNA
0	0	5	3	4	NM_132695.1	CG10993-RA (CG10993), mRNA
0	0	4	4	5	NM_135604.2	CG12299-RA (CG12299), mRNA
0	0	4	3	22	NM_170139.1	CG31132-RA (BRWD3), mRNA
0	0	4	2	7	NM_057923.2	CG6708-RA (Osbp), mRNA
0	0	3	3	2	NM_136889.2	CG8878-RA, transcript variant A (CG8878), mRNA
0	0	3	3	2	NM_206099.1	CG8878-RB, transcript variant B (CG8878), mRNA
0	0	2	7	20	NM_140110.2	CG8108-RB, transcript variant B (CG8108), mRNA
0	0	2	7	20	NM_168386.1	CG8108-RA, transcript variant A (CG8108), mRNA
0	0	2	6	10	NM_132074.1	CG3585-RA (CG3585), mRNA
0	0	2	5	2	NM_131954.1	CG7024-RA (CG7024), mRNA
0	0	2	4	8	NM_142397.1	CG14318-RA (CG14318), mRNA
0	0	2	3	9	NM_001042802.1	CG34104-RA, transcript variant A (CG34104), mRNA
0	0	2	3	9	NM_001042801.1	CG34104-RB, transcript variant B (CG34104), mRNA
0	0	2	3	6	NM_057676.3	CG11312-RA (insc), mRNA
0	0	2	3	4	NM_170027.2	CG31158-RA, transcript variant A (CG31158), mRNA
0	0	2	3	4	NM_176541.1	CG31158-RB, transcript variant B (CG31158), mRNA
0	0	2	2	6	NM_058115.3	CG2859-RA (Taf10), mRNA
0	0	2	2	5	NM_057545.3	CG14513-RA (yemalpha), mRNA
0	0	2	2	5	NM_057773.3	CG7538-RA (Mcm2), mRNA
0	0	2	2	4	NM_079471.2	CG18023-RA, transcript variant A (Eip78C), mRNA
0	0	2	2	4	NM_168892.1	CG18023-RB, transcript variant B (Eip78C), mRNA
0	0	2	2	3	NM_167553.1	CG9059-RA, transcript variant A (CG9059), mRNA

Back