NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4897R-2

Symbol RpL7 Full Name Ribosomal protein L7

CG No CG4897 Old CG No CG4897

Synonyms L7, CG4897, unnamed, BEST:GH04411, RpL7

Accession No (Link to NCBI) NM_078807.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGGCCTTTGTCGTCCGTATC

Primer Seq. 3'

aatctagaggtaccCATCTTGCGCAGCAGACGGTT

Predicted Fragment Size

IR fragment full Seq

0001 TTGGCCTTTG TCGTCCGTAT CCGCGGTATC AACAAGGTGG CTCCCAAGGT CCGCAAGGTT 
0061 CTGCAGCTGT TCCGTCTGAG GCAGATCAAC AACGGTGTGT TCATCAAGCT GAACAAGGCC 
0121 ACCATCAACC ATGCTGCGCA TCGCCGAGCC CTACATCACC TGGGGCTATC CCAATCTGAA 
0181 GTCCGTGCGG GAGCTGATCT ACAAGCGTGG ATTCGTGAAG CATAACCGCC AGGCGCGTGC 
0241 CCATCACCGA TAACTTCGTG ATCGAGCGGA AgcTGCTNGT CAGGGCGCAC CAAATTCAGT 
0301 GCGTCGAGGA TCTTGTCCAT GAGATCTTCC ACCGTGGGGC CCAACTTCAA GTACGCCTCC 
0361 AACTTCCTGT GGCCCTTCAA GCTAAACACC CCCACCGGCG GCTGGCGCAA GAAGGCCAAC 
0421 CATTATGTCA ACGGTGGTGA CTTCGGCAAC CGCGAGGACC AGATCAACCG TCTGCTGCGC 
0481 AAGATG

in silico PCR Fragment

0001 TTGGCCTTTG TCGTCCGTAT CCGCGGTATC AACAAGGTGG CTCCCAAGGT CCGCAAGGTT 
0061 CTGCAGCTGT TCCGTCTGAG GCAGATCAAC AACGGTGTGT TCATCAAGCT GAACAAGGCC 
0121 ACCATCAA-C ATGCTGCGCA TCGCCGAGCC CTACATCACC TGGGGCTATC CCAATCTGAA 
0181 GTCCGTGCGG GAGCTGATCT ACAAGCGTGG ATTCGTGAAG CATAACCGCC A-GCGCGTGC 
0241 CCATCACCGA TAACTTCGTG ATCGAGCGGA AGCTGC--GT CA-GGCGCAC CAAATTCAGT 
0301 GCGTCGAGGA TCTTGTCCAT GAGATCTT-C ACCGTGGGGC CCAACTTCAA GTACGCCTCC 
0361 AACTTCCTGT GGCCCTTCAA GCTAAACACC CCCACCGGCG GCTGGCGCAA GAAGGCCAAC 
0421 CATTATGTCA ACGGTGGTGA CTTCGGCAAC CGCGAGGACC AGATCAACCG TCTGCTGCGC 
0481 AAGATG

Assemble Data


4897R-2.IR_full       1   TTGGCCTTTGTCGTCCGTATCCGCGGTATCAACAAGGTGGCTCCCAAGGTCCGCAAGGTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4897R-2.in silico     1   TTGGCCTTTGTCGTCCGTATCCGCGGTATCAACAAGGTGGCTCCCAAGGTCCGCAAGGTT 60


4897R-2.IR_full       61  CTGCAGCTGTTCCGTCTGAGGCAGATCAACAACGGTGTGTTCATCAAGCTGAACAAGGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4897R-2.in silico     61  CTGCAGCTGTTCCGTCTGAGGCAGATCAACAACGGTGTGTTCATCAAGCTGAACAAGGCC 120


4897R-2.IR_full       121 ACCATCAACCATGCTGCGCATCGCCGAGCCCTACATCACCTGGGGCTATCCCAATCTGAA 180
                          |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
4897R-2.in silico     121 ACCATCAA-CATGCTGCGCATCGCCGAGCCCTACATCACCTGGGGCTATCCCAATCTGAA 180


4897R-2.IR_full       181 GTCCGTGCGGGAGCTGATCTACAAGCGTGGATTCGTGAAGCATAACCGCCAGGCGCGTGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
4897R-2.in silico     181 GTCCGTGCGGGAGCTGATCTACAAGCGTGGATTCGTGAAGCATAACCGCCA-GCGCGTGC 240


4897R-2.IR_full       241 CCATCACCGATAACTTCGTGATCGAGCGGAAGCTGCTNGTCAGGGCGCACCAAATTCAGT 300
                          ||||||||||||||||||||||||||||||||||||  |||| |||||||||||||||||
4897R-2.in silico     241 CCATCACCGATAACTTCGTGATCGAGCGGAAGCTGC--GTCA-GGCGCACCAAATTCAGT 300


4897R-2.IR_full       301 GCGTCGAGGATCTTGTCCATGAGATCTTCCACCGTGGGGCCCAACTTCAAGTACGCCTCC 360
                          |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
4897R-2.in silico     301 GCGTCGAGGATCTTGTCCATGAGATCTT-CACCGTGGGGCCCAACTTCAAGTACGCCTCC 360


4897R-2.IR_full       361 AACTTCCTGTGGCCCTTCAAGCTAAACACCCCCACCGGCGGCTGGCGCAAGAAGGCCAAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4897R-2.in silico     361 AACTTCCTGTGGCCCTTCAAGCTAAACACCCCCACCGGCGGCTGGCGCAAGAAGGCCAAC 420


4897R-2.IR_full       421 CATTATGTCAACGGTGGTGACTTCGGCAACCGCGAGGACCAGATCAACCGTCTGCTGCGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4897R-2.in silico     421 CATTATGTCAACGGTGGTGACTTCGGCAACCGCGAGGACCAGATCAACCGTCTGCTGCGC 480


4897R-2.IR_full       481 AAGATG 486
                          ||||||
4897R-2.in silico     481 AAGATG 486

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	462	0	0	0	NM_078807.1	CG4897-RA (RpL7), mRNA
0.21	1	4	3	2	NM_142535.2	CG11703-RA (CG11703), mRNA
0	0	4	3	6	NM_141490.1	CG2943-RA (CG2943), mRNA
0	0	3	4	7	NM_169435.1	CG6946-RC, transcript variant C (glo), mRNA
0	0	3	4	7	NM_141863.1	CG6946-RA, transcript variant A (glo), mRNA
0	0	3	4	7	NM_169434.1	CG6946-RB, transcript variant B (glo), mRNA
0	0	3	4	4	NM_133004.1	CG8211-RA (CG8211), mRNA
0	0	3	3	3	NM_165256.1	CG31789-RA (CG31789), mRNA
0	0	1	4	9	NM_135176.2	CG9506-RA (slam), mRNA
0	0	1	3	6	NM_142622.1	CG4360-RA (CG4360), mRNA
0	0	1	3	4	NM_080214.2	CG10913-RA (Spn6), mRNA
0	0	1	3	3	NM_142495.1	CG14299-RA, transcript variant A (CG14299), mRNA
0	0	1	3	2	NM_176246.1	CG33143-RC, transcript variant C (CG33143), mRNA
0	0	1	3	2	NM_176247.1	CG33143-RB, transcript variant B (CG33143), mRNA
0	0	1	3	2	NM_169624.1	CG4247-RA, transcript variant A (mRpS10), mRNA
0	0	1	3	2	NM_079631.3	CG4247-RB, transcript variant B (mRpS10), mRNA
0	0	1	2	5	NM_140447.1	CG9598-RA (CG9598), mRNA
0	0	1	2	4	NM_140761.2	CG7441-RA (CG7441), mRNA
0	0	1	2	3	NM_001043329.1	CG8144-RG, transcript variant G (ps), mRNA
0	0	1	2	3	NM_001043328.1	CG8144-RK, transcript variant K (ps), mRNA
0	0	1	2	3	NM_176579.1	CG33203-RC (CG33203), mRNA
0	0	1	2	3	NM_079738.2	CG6768-RA (DNApol-epsilon), mRNA
0	0	1	2	3	NM_001043331.1	CG8144-RH, transcript variant H (ps), mRNA
0	0	1	2	3	NM_001043327.1	CG8144-RF, transcript variant F (ps), mRNA
0	0	1	2	3	NM_137243.2	CG8431-RA (Aats-cys), mRNA
0	0	1	2	3	NM_142213.1	CG14870-RA (CG14870), mRNA
0	0	1	2	2	NM_169285.3	CG8144-RC, transcript variant C (ps), mRNA
0	0	1	2	2	NM_001043330.1	CG8144-RJ, transcript variant J (ps), mRNA
0	0	1	2	2	NM_169284.2	CG8144-RD, transcript variant D (ps), mRNA
0	0	1	2	2	NM_169283.1	CG8144-RB, transcript variant B (ps), mRNA

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