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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4838R-2 |
| Symbol |
beat-Ic |
Full Name |
beaten path Ic |
| CG No |
CG4838 |
Old CG No |
CG4838 |
| Synonyms |
beat-C, beat Ic, CT15531, BG:DS00913.1, CG4838, beat-Ic |
| Accession No (Link to NCBI) |
NM_078856.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGACGACTATTCCGGGGGCAA |
| Primer Seq. 3' |
aatctagaggtaccCAGCTCAACCACCTCCATTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGACGACTAT TCCGGGGGCA AGCACCCGTC ACATGCAAAT GGCCAATCGG TGGAGGACGG
0061 AAAAGGACGT TGGATCCCAT GGTTCCCAAA GTGGGCGTAG CTGCATCTGC TGGATATCCG
0121 TGGTACTCCT ACTCATTCTG GTGCCTGATT TTATTGAAGC ACTCAAGGAT GTGTCGGTAA
0181 TGATACCGCA GGCGGTGNAA ACGTGGCAGC AATGCGCNTA TTCACTTGTA ATTACGATAT
0241 GGAAAACGAT ACTCTATATT CGGTTAAATG GTATAAGGGC AAGCGTGAGT TTTATCGCTA
0301 TACGCCCAAG GAGAATCCGG CGATGAAGGT CTTTGCCATG ACAAGTGGTC TCAATGTCGA
0361 GCGCAACCTT TCGAATCAGA GCCACGTCGT CCTGCAGTCG GTGCCGCTGA ATATTTCGGG
0421 GAAATTTACA TGCGAAATAT CGGTGGAGGC GCCCACTTTT CAAACGGCCA TGGTGTCCGG
0481 CGAAATGGAG GTGGTTGAGC TG |
| in silico PCR Fragment |
0001 AGACGACTAT TCCGGGGGCA AGCACCCGTC ACATGCAAAT GGCCAATCGG TGGAGGACGG
0061 AAAAGGACGT TGGATCCCAT GGTTCCCAAA GTGGGCGTAG CTGCATCTGC TGGATATCCG
0121 TGGTACTCCT ACTCATTCTG GTGCCTGATT TTATTGAAGC ACTCAAGGAT GTGTCGGTAA
0181 TGATACCGCA GGCGGTG-AA ACGTGGCAGC AATGCGC-TA TTCACTTGTA ATTACGATAT
0241 GGAAAACGAT ACTCTATATT CGGTTAAATG GTATAAGGGC AAGCGTGAGT TTTATCGCTA
0301 TACGCCCAAG GAGAATCCGG CGATGAAGGT CTTTGCCATG ACAAGTGGTC TCAATGTCGA
0361 GCGCAACCTT TCGAATCAGA GCCACGTCGT CCTGCAGTCG GTGCCGCTGA ATATTTCGGG
0421 GAAATTTACA TGCGAAATAT CGGTGGAGGC GCCCACTTTT CAAACGGCCA TGGTGTCCGG
0481 CGAAATGGAG GTGGTTGAGC TG |
| Assemble Data |
4838R-2.IR_full 1 AGACGACTATTCCGGGGGCAAGCACCCGTCACATGCAAATGGCCAATCGGTGGAGGACGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 1 AGACGACTATTCCGGGGGCAAGCACCCGTCACATGCAAATGGCCAATCGGTGGAGGACGG 60
4838R-2.IR_full 61 AAAAGGACGTTGGATCCCATGGTTCCCAAAGTGGGCGTAGCTGCATCTGCTGGATATCCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 61 AAAAGGACGTTGGATCCCATGGTTCCCAAAGTGGGCGTAGCTGCATCTGCTGGATATCCG 120
4838R-2.IR_full 121 TGGTACTCCTACTCATTCTGGTGCCTGATTTTATTGAAGCACTCAAGGATGTGTCGGTAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 121 TGGTACTCCTACTCATTCTGGTGCCTGATTTTATTGAAGCACTCAAGGATGTGTCGGTAA 180
4838R-2.IR_full 181 TGATACCGCAGGCGGTGNAAACGTGGCAGCAATGCGCNTATTCACTTGTAATTACGATAT 240
||||||||||||||||| ||||||||||||||||||| ||||||||||||||||||||||
4838R-2.in silico 181 TGATACCGCAGGCGGTG-AAACGTGGCAGCAATGCGC-TATTCACTTGTAATTACGATAT 240
4838R-2.IR_full 241 GGAAAACGATACTCTATATTCGGTTAAATGGTATAAGGGCAAGCGTGAGTTTTATCGCTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 241 GGAAAACGATACTCTATATTCGGTTAAATGGTATAAGGGCAAGCGTGAGTTTTATCGCTA 300
4838R-2.IR_full 301 TACGCCCAAGGAGAATCCGGCGATGAAGGTCTTTGCCATGACAAGTGGTCTCAATGTCGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 301 TACGCCCAAGGAGAATCCGGCGATGAAGGTCTTTGCCATGACAAGTGGTCTCAATGTCGA 360
4838R-2.IR_full 361 GCGCAACCTTTCGAATCAGAGCCACGTCGTCCTGCAGTCGGTGCCGCTGAATATTTCGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 361 GCGCAACCTTTCGAATCAGAGCCACGTCGTCCTGCAGTCGGTGCCGCTGAATATTTCGGG 420
4838R-2.IR_full 421 GAAATTTACATGCGAAATATCGGTGGAGGCGCCCACTTTTCAAACGGCCATGGTGTCCGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4838R-2.in silico 421 GAAATTTACATGCGAAATATCGGTGGAGGCGCCCACTTTTCAAACGGCCATGGTGTCCGG 480
4838R-2.IR_full 481 CGAAATGGAGGTGGTTGAGCTG 502
||||||||||||||||||||||
4838R-2.in silico 481 CGAAATGGAGGTGGTTGAGCTG 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078856.2 |
CG4838-RA (beat-Ic), mRNA |
| 0 |
0 |
10 |
13 |
14 |
NM_078855.2 |
CG7644-RA (beat-Ib), mRNA |
| 0 |
0 |
4 |
4 |
14 |
NM_058160.3 |
CG4846-RA (beat-Ia), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137615.2 |
CG11048-RA (CG11048), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143571.2 |
CG15544-RA (CG15544), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_057597.2 |
CG10719-RA, transcript variant A (brat), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_134302.1 |
CG10719-RB, transcript variant B (brat), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206004.1 |
CG10719-RC, transcript variant C (brat), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079113.2 |
CG4012-RA (gek), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142177.2 |
CG6563-RA, transcript variant A (Art3), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169623.2 |
CG6563-RB, transcript variant B (Art3), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_001038902.1 |
CG33993-RA (CG33993), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_170141.1 |
CG6454-RA, transcript variant A (CG6454), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_142992.1 |
CG6454-RB, transcript variant B (CG6454), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166147.1 |
CG8428-RE, transcript variant E (spin), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166145.1 |
CG8428-RA, transcript variant A (spin), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_080084.2 |
CG8428-RD, transcript variant D (spin), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166144.1 |
CG8428-RC, transcript variant C (spin), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166146.1 |
CG8428-RB, transcript variant B (spin), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_135593.2 |
CG7296-RA (CG7296), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_166127.1 |
CG18255-RF, transcript variant F (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_143579.2 |
CG15548-RA (CG15548), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078689.2 |
CG14228-RA (Mer), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_165169.3 |
CG5996-RB, transcript variant B (trpgamma), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_135958.3 |
CG5996-RA, transcript variant A (trpgamma), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_139875.3 |
CG7546-RA, transcript variant A (CG7546), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_168217.2 |
CG7546-RB, transcript variant B (CG7546), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_079640.2 |
CG6226-RA (FK506-bp1), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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