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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4830R-3 
 Symbol CG4830  Full Name CG4830 
 CG No CG4830  Old CG No CG4830 
 Synonyms CG4830 
 Accession No (Link to NCBI) NM_141903.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCAAGTTACCTTGCAGCTACG 
 Primer Seq. 3' 
aatctagaggtaccCAGATCCTGAATGCTAAGGAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCAAGTTACC TTGCAGCTAC GATGCGGAGA ACAAGATCTG GAAGGGTATT CCGCAAAACA 
0061 ACCCCTTCAA GCCGGAGACC TCCCTGGGTC GCATCATCTT CAAGAACATG AGGAACTGGC 
0121 CCAAGAATGT GTGTCAGATT TCCGACTCCG AGGGCGTGGA GGTCACCTTC GGACAGGCTC 
0181 TCACTTGGGC TATTCGCATT GCCCAGCAAC TGAAGAGTCG CGGTCTCGAC CACAAGGGTA 
0241 TCATTGGTAT CTCTGCCAGG AACACCACCT ACATTATGCC CACAGCCGTG GCCTGTTTCT 
0301 TCCATGGCAC TCCCTTCCAG TCGGCCAATC CAATTCTCGA GGAATCTACT CTTAAGCACC 
0361 TGTACAACAT TTCCAAGCCG AAGATTATCT TCACCGATGC CGATCACTAC GACAAACTGT 
0421 ACTCGGCTAC CAGCGCGTTC AAGCCAGAGA TTATCCTAAC CACCGGAACT AAGGATGGTG 
0481 TCCTTAGCAT TCAGGATCTG  
 in silico PCR Fragment 
0001 CCAAGTTACC TTGCAGCTAC GATGCGGAGA ACAAGATCTG GAAGGGTATT CCGCAAAACA 
0061 ACCCCTTCAA GCCGGAGACC TCCCTGGGTC GCATCATCTT CAAGAACATG AGGAACTGGC 
0121 CCAAGAATGT GTGTCAGATT TCCGACTCCG AGGGCGTGGA GGTCACCTTC GGACAGGCTC 
0181 TCACTTGGGC TATTCGCATT GCCCAGCAAC TGAAGAGTCG CGGTCTCGAC CACAAGGATA 
0241 TCATTGGTAT CTCTGCCAGG AACACCACCT ACATTATGCC CACAGCCGTG GCCTGTTTCT 
0301 TCCATGGCAC TCCCTTCCAG TCGGCCAATC CAATTCTCGA GGAATCTACT CTTAAGCACC 
0361 TGTACAACAT TTCCAAGCCG AAGATTATCT TCACCGATGC CGATCACTAC GACAAACTGT 
0421 ACTCGGCTAC CAGCGCGTTC AAGCCAGAGA TTATCCTAAC CACCGGAACT AAGGATGGTG 
0481 TCCTTAGCAT TCAGGATCTG  
 Assemble Data 

4830R-3.IR_full       1   CCAAGTTACCTTGCAGCTACGATGCGGAGAACAAGATCTGGAAGGGTATTCCGCAAAACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     1   CCAAGTTACCTTGCAGCTACGATGCGGAGAACAAGATCTGGAAGGGTATTCCGCAAAACA 60


4830R-3.IR_full       61  ACCCCTTCAAGCCGGAGACCTCCCTGGGTCGCATCATCTTCAAGAACATGAGGAACTGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     61  ACCCCTTCAAGCCGGAGACCTCCCTGGGTCGCATCATCTTCAAGAACATGAGGAACTGGC 120


4830R-3.IR_full       121 CCAAGAATGTGTGTCAGATTTCCGACTCCGAGGGCGTGGAGGTCACCTTCGGACAGGCTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     121 CCAAGAATGTGTGTCAGATTTCCGACTCCGAGGGCGTGGAGGTCACCTTCGGACAGGCTC 180


4830R-3.IR_full       181 TCACTTGGGCTATTCGCATTGCCCAGCAACTGAAGAGTCGCGGTCTCGACCACAAGGGTA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
4830R-3.in silico     181 TCACTTGGGCTATTCGCATTGCCCAGCAACTGAAGAGTCGCGGTCTCGACCACAAGGATA 240


4830R-3.IR_full       241 TCATTGGTATCTCTGCCAGGAACACCACCTACATTATGCCCACAGCCGTGGCCTGTTTCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     241 TCATTGGTATCTCTGCCAGGAACACCACCTACATTATGCCCACAGCCGTGGCCTGTTTCT 300


4830R-3.IR_full       301 TCCATGGCACTCCCTTCCAGTCGGCCAATCCAATTCTCGAGGAATCTACTCTTAAGCACC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     301 TCCATGGCACTCCCTTCCAGTCGGCCAATCCAATTCTCGAGGAATCTACTCTTAAGCACC 360


4830R-3.IR_full       361 TGTACAACATTTCCAAGCCGAAGATTATCTTCACCGATGCCGATCACTACGACAAACTGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     361 TGTACAACATTTCCAAGCCGAAGATTATCTTCACCGATGCCGATCACTACGACAAACTGT 420


4830R-3.IR_full       421 ACTCGGCTACCAGCGCGTTCAAGCCAGAGATTATCCTAACCACCGGAACTAAGGATGGTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4830R-3.in silico     421 ACTCGGCTACCAGCGCGTTCAAGCCAGAGATTATCCTAACCACCGGAACTAAGGATGGTG 480


4830R-3.IR_full       481 TCCTTAGCATTCAGGATCTG 500
                          ||||||||||||||||||||
4830R-3.in silico     481 TCCTTAGCATTCAGGATCTG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141903.2  CG4830-RA (CG4830), mRNA 
0.62   3  16  18  19  NM_136935.1  CG8834-RA (CG8834), mRNA 
0   0  6  6  8  NM_142575.1  CG11453-RA (CG11453), mRNA 
0   0  6  2  4  NM_140381.1  CG14110-RA (CG14110), mRNA 
0   0  5  2  6  NM_141742.2  CG6312-RA (Rfx), mRNA 
0   0  4  4  2  NM_137883.1  CG13535-RA (CG13535), mRNA 
0   0  3  7  2  NM_131970.1  CG6903-RA (CG6903), mRNA 
0   0  1  4  7  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   0  1  4  6  NM_166129.2  CG18255-RD, transcript variant D (Strn-Mlck), mRNA 
0   0  1  4  5  NM_166126.1  CG18255-RC, transcript variant C (Strn-Mlck), mRNA 
0   0  1  4  4  NM_205957.1  CG33304-RA (rho-5), mRNA 
0   0  1  4  4  NM_166130.1  CG18255-RE, transcript variant E (Strn-Mlck), mRNA 
0   0  1  3  6  NM_001042907.1  CG9243-RB, transcript variant B (CG9243), mRNA 
0   0  1  3  6  NM_136252.2  CG9243-RA, transcript variant A (CG9243), mRNA 
0   0  1  3  2  NM_137623.2  CG9143-RA (CG9143), mRNA 
0   0  1  3  2  NM_078724.4  CG13948-RA (Gr21a), mRNA 
0   0  1  2  3  NM_139457.1  CG13801-RA (CG13801), mRNA 
0   0  0  5  4  NM_057269.2  CG10236-RA (LanA), mRNA 
0   0  0  5  3  NM_057440.3  CG7811-RA (b), mRNA 
0   0  0  5  2  NM_137156.2  CG10242-RA (Cyp6a23), mRNA 
0   0  0  4  5  NM_164430.2  CG31666-RD, transcript variant D (CG31666), mRNA 
0   0  0  4  5  NM_134766.3  CG31666-RA, transcript variant A (CG31666), mRNA 
0   0  0  4  5  NM_164428.2  CG31666-RB, transcript variant B (CG31666), mRNA 
0   0  0  4  5  NM_164429.1  CG31666-RC, transcript variant C (CG31666), mRNA 
0   0  0  4  4  NM_079106.2  CG4817-RA (Ssrp), mRNA 
0   0  0  4  2  NM_168608.1  CG16959-RB, transcript variant B (CG16959), mRNA 
0   0  0  4  2  NM_140493.2  CG16959-RA, transcript variant A (CG16959), mRNA 
0   0  0  3  11  NM_132074.1  CG3585-RA (CG3585), mRNA 
0   0  0  3  7  NM_166543.1  CG13521-RB, transcript variant B (robo), mRNA 
0   0  0  3  7  NM_057551.3  CG13521-RA, transcript variant A (robo), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
++ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
++ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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