NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4827R-3

Symbol CG4827 Full Name CG4827

CG No CG4827 Old CG No CG4827

Synonyms CG4827

Accession No (Link to NCBI) NM_137373.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCTGCCCGTGGTCTCCTTATC

Primer Seq. 3'

aatctagaggtaccGCCGCCATTTCCGGAACATCG

Predicted Fragment Size

IR fragment full Seq

0001 CCTGCCCGTG GTCTCCTTAT CGGATCATGC TGCCTACTGG CTCTACTGAT TCATCCTGTG 
0061 CAGCCGAATC CCATTGCACC CCGGGCGGAA GTTGCCGCCG AGTTCATCAT TCTGCACAAC 
0121 AATGACATGC ACGCCCGATT CGAGCAGACG AGTGTGACCA GTGGCACCTG CTCCAAGGAG 
0181 GAGGCCAATA CGGATCAGTG CTACGGAGGA TTCGCCAGGG TGGCATACGA GGTGCGAAAG 
0241 TATCGCAAGG AGGCGGAGGA GGGTGGAACT CCAGTGTTTT ATCTCAATGC AGGTGACACC 
0301 TATACCGGAA CTGCTTGGTT CACCGTCTAT AAGGACAAGA TCGCCAGTGC TTTCCTCAAC 
0361 AAGCTATCGC CAGATGCTAT TTCTCTGGGC AACCATGAGT TTGATCAGAA TGTTGAGGGT 
0421 TTGGTGCCCT TCCTAAATGC CGTGGAATTC CCTGTTTTGG CCTGTAATCT GAACCTCACC 
0481 GATGTTCCGG AAATGNGCGG c

in silico PCR Fragment

0001 CCTGCCCGTG GTCTCCTTAT CGGATCATGC TGCCTACTGG CTCTACTGAT TCATCCTGTG 
0061 CAGCCGAATC CCATTGCACC CCGGGCGGAA GTTGCCGCCG AGTTCATCAT TCTGCACAAC 
0121 AATGACATGC ACGCCCGATT CGAGCAGACG AGTGTGACCA GTGGCACCTG CTCCAAGGAG 
0181 GAGGCCAATA CGGATCAGTG CTACGGAGGA TTCGCCAGGG TGGCATACGA GGTGCGAAAG 
0241 TATCGCAAGG AGGCGGAGGA GGGTGGAACT CCAGTGTTTT ATCTCAATGC AGGTGACACC 
0301 TATACCGGAA CTGCTTGGTT CACCGTCTAT AAGGACAAGA TCGCCAGTGC TTTCCTCAAC 
0361 AAGCTATCGC CAGATGCTAT TTCTCTGGGC AACCATGAGT TTGATCAGAA TGTTGAGGGT 
0421 TTGGTGCCCT TCCTAAATGC CGTGGAATTC CCTGTTTTGG CCTGTAATCT GAACCTCACC 
0481 GATGTTCCGG AAATG-GCGG C

Assemble Data


4827R-3.IR_full       1   CCTGCCCGTGGTCTCCTTATCGGATCATGCTGCCTACTGGCTCTACTGATTCATCCTGTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     1   CCTGCCCGTGGTCTCCTTATCGGATCATGCTGCCTACTGGCTCTACTGATTCATCCTGTG 60


4827R-3.IR_full       61  CAGCCGAATCCCATTGCACCCCGGGCGGAAGTTGCCGCCGAGTTCATCATTCTGCACAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     61  CAGCCGAATCCCATTGCACCCCGGGCGGAAGTTGCCGCCGAGTTCATCATTCTGCACAAC 120


4827R-3.IR_full       121 AATGACATGCACGCCCGATTCGAGCAGACGAGTGTGACCAGTGGCACCTGCTCCAAGGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     121 AATGACATGCACGCCCGATTCGAGCAGACGAGTGTGACCAGTGGCACCTGCTCCAAGGAG 180


4827R-3.IR_full       181 GAGGCCAATACGGATCAGTGCTACGGAGGATTCGCCAGGGTGGCATACGAGGTGCGAAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     181 GAGGCCAATACGGATCAGTGCTACGGAGGATTCGCCAGGGTGGCATACGAGGTGCGAAAG 240


4827R-3.IR_full       241 TATCGCAAGGAGGCGGAGGAGGGTGGAACTCCAGTGTTTTATCTCAATGCAGGTGACACC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     241 TATCGCAAGGAGGCGGAGGAGGGTGGAACTCCAGTGTTTTATCTCAATGCAGGTGACACC 300


4827R-3.IR_full       301 TATACCGGAACTGCTTGGTTCACCGTCTATAAGGACAAGATCGCCAGTGCTTTCCTCAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     301 TATACCGGAACTGCTTGGTTCACCGTCTATAAGGACAAGATCGCCAGTGCTTTCCTCAAC 360


4827R-3.IR_full       361 AAGCTATCGCCAGATGCTATTTCTCTGGGCAACCATGAGTTTGATCAGAATGTTGAGGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     361 AAGCTATCGCCAGATGCTATTTCTCTGGGCAACCATGAGTTTGATCAGAATGTTGAGGGT 420


4827R-3.IR_full       421 TTGGTGCCCTTCCTAAATGCCGTGGAATTCCCTGTTTTGGCCTGTAATCTGAACCTCACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4827R-3.in silico     421 TTGGTGCCCTTCCTAAATGCCGTGGAATTCCCTGTTTTGGCCTGTAATCTGAACCTCACC 480


4827R-3.IR_full       481 GATGTTCCGGAAATGNGCGGC 501
                          ||||||||||||||| |||||
4827R-3.in silico     481 GATGTTCCGGAAATG-GCGGC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137373.2	CG4827-RA (CG4827), mRNA
0.41	2	24	31	69	NM_137374.2	CG30104-RA, transcript variant A (CG30104), mRNA
0.41	2	24	31	69	NM_166232.1	CG30104-RB, transcript variant B (CG30104), mRNA
0.2	1	2	2	2	NM_140922.2	CG7348-RA (CG7348), mRNA
0	0	3	2	2	NM_164517.1	CG8817-RC, transcript variant C (lilli), mRNA
0	0	3	2	2	NM_164516.1	CG8817-RA, transcript variant A (lilli), mRNA
0	0	3	2	2	NM_078740.2	CG8817-RB, transcript variant B (lilli), mRNA
0	0	3	2	2	NM_164707.1	CG11199-RB, transcript variant B (Liprin-alpha), mRNA
0	0	3	2	2	NM_135223.2	CG11199-RA, transcript variant A (Liprin-alpha), mRNA
0	0	2	2	2	NM_137918.2	CG12192-RA (Klp59D), mRNA
0	0	1	4	3	NM_134677.1	CG13690-RA (CG13690), mRNA
0	0	1	4	2	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	1	2	6	NM_134690.2	CG2807-RA (CG2807), mRNA
0	0	1	2	5	NM_166535.1	CG4444-RA (px), mRNA
0	0	1	2	5	NM_079007.2	CG17716-RA (fas), mRNA
0	0	1	2	3	NM_132789.2	CG6170-RA, transcript variant A (HDAC6), mRNA
0	0	1	2	3	NM_167437.1	CG6170-RC, transcript variant C (HDAC6), mRNA
0	0	1	2	3	NM_167436.1	CG6170-RB, transcript variant B (HDAC6), mRNA
0	0	1	2	3	NM_078616.3	CG10524-RA (Pkcdelta), mRNA
0	0	1	2	2	NM_166263.1	CG5753-RB, transcript variant B (stau), mRNA
0	0	1	2	2	NM_057403.3	CG5753-RA, transcript variant A (stau), mRNA
0	0	0	9	9	NM_057242.3	CG7765-RA (Khc), mRNA
0	0	0	7	6	NM_143104.1	CG11856-RA (Nup358), mRNA
0	0	0	5	5	NM_133106.2	CG7288-RA (CG7288), mRNA
0	0	0	5	4	NM_136515.2	CG8709-RA (CG8709), mRNA
0	0	0	4	8	NM_001014718.1	CG33548-RB, transcript variant B (msta), mRNA
0	0	0	4	5	NM_169228.1	CG31258-RA (CG31258), mRNA
0	0	0	4	4	NM_135235.2	CG10805-RA (CG10805), mRNA
0	0	0	4	4	NM_137191.1	CG18625-RA (CG18625), mRNA
0	0	0	4	3	NM_136900.1	CG8859-RA (Cyp6g2), mRNA

Back