National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4824R-5 
 Symbol BicC  Full Name Bicaudal C 
 CG No CG4824  Old CG No CG4824 
 Synonyms Bic-C, BG:DS00913.2, br34, l(2)br34, CG4824, BicC 
 Accession No (Link to NCBI) NM_057517.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTTGTCCTGTGCCTCTTTCA 
 Primer Seq. 3' 
aatctagaggtaccGTCAAGACTGCTAAGGATGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGTTGTCCTG TGCCTCTTTC AATAAACTGA TGTATCCGTC GGCCGCAGAT GTTGCCAAAC 
0061 CGCCAATGGT GGGCTTGGAG GTGGAAGCTG GATCCATTGG ATCCCTGTCC AGTCTTCACG 
0121 CCCTTCCATC GACCACCTCG GTGGGCAGTG GAGCCCCCAG CGAGACGCAG AGCGAGNATC 
0181 TCCTCCGTGG ATAGCGATTG GAGTGACATA CGCGCCATCG CCATGAAGTT GGGCGTTCAA 
0241 AACCCAGACG ATCTCCACAC GGAACGTTTC AAGGTGGATC GACAGAAATT GGAACAGTTA 
0301 ATAAAAGCGG AGAGCTCCAT TGAGGGAATG AATGGAGCTG AATATTTTTT CCATGACATC 
0361 ATGAACACTA CGGATACTTA TGTGAGCTGG CCTTGCAGAC TCAAAATTGG TGCCAAGAGC 
0421 AAGAAGGATC CCCATGTGCG AATTGTGGGA AAGGTGGATC AGGTTCAGCG GGCAAAGGAG 
0481 CGCATCCTTA GCAGTCTTGA C 
 in silico PCR Fragment 
0001 TGTTGTCCTG TGCCTCTTTC AATAAACTGA TGTATCCGTC GGCCGCAGAT GTTGCCAAAC 
0061 CGCCAATGGT GGGCTTGGAG GTGGAAGCTG GATCCATTGG ATCCCTGTCC AGTCTTCACG 
0121 CCCTTCCATC GACCACCTCG GTGGGCAGTG GAGCCCCCAG CGAGACGCAG AGCGAG-ATC 
0181 TCCTCCGTGG ATAGCGATTG GAGTGACATA CGCGCCATCG CCATGAAGTT GGGCGTTCAA 
0241 AACCCAGACG ATCTCCACAC GGAACGTTTC AAGGTGGATC GACAGAAATT GGAACAGTTA 
0301 ATAAAAGCGG AGAGCTCCAT TGAGGGAATG AATGGAGCTG AATATTTTTT CCATGACATC 
0361 ATGAACACTA CGGATACTTA TGTGAGCTGG CCTTGCAGAC TCAAAATTGG TGCCAAGAGC 
0421 AAGAAGGATC CCCATGTGCG AATTGTGGGA AAGGTGGATC AGGTTCAGCG GGCAAAGGAG 
0481 CGCATCCTTA GCAGTCTTGA C 
 Assemble Data 

4824R-5.IR_full       1   TGTTGTCCTGTGCCTCTTTCAATAAACTGATGTATCCGTCGGCCGCAGATGTTGCCAAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     1   TGTTGTCCTGTGCCTCTTTCAATAAACTGATGTATCCGTCGGCCGCAGATGTTGCCAAAC 60


4824R-5.IR_full       61  CGCCAATGGTGGGCTTGGAGGTGGAAGCTGGATCCATTGGATCCCTGTCCAGTCTTCACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     61  CGCCAATGGTGGGCTTGGAGGTGGAAGCTGGATCCATTGGATCCCTGTCCAGTCTTCACG 120


4824R-5.IR_full       121 CCCTTCCATCGACCACCTCGGTGGGCAGTGGAGCCCCCAGCGAGACGCAGAGCGAGNATC 180
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
4824R-5.in silico     121 CCCTTCCATCGACCACCTCGGTGGGCAGTGGAGCCCCCAGCGAGACGCAGAGCGAG-ATC 180


4824R-5.IR_full       181 TCCTCCGTGGATAGCGATTGGAGTGACATACGCGCCATCGCCATGAAGTTGGGCGTTCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     181 TCCTCCGTGGATAGCGATTGGAGTGACATACGCGCCATCGCCATGAAGTTGGGCGTTCAA 240


4824R-5.IR_full       241 AACCCAGACGATCTCCACACGGAACGTTTCAAGGTGGATCGACAGAAATTGGAACAGTTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     241 AACCCAGACGATCTCCACACGGAACGTTTCAAGGTGGATCGACAGAAATTGGAACAGTTA 300


4824R-5.IR_full       301 ATAAAAGCGGAGAGCTCCATTGAGGGAATGAATGGAGCTGAATATTTTTTCCATGACATC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     301 ATAAAAGCGGAGAGCTCCATTGAGGGAATGAATGGAGCTGAATATTTTTTCCATGACATC 360


4824R-5.IR_full       361 ATGAACACTACGGATACTTATGTGAGCTGGCCTTGCAGACTCAAAATTGGTGCCAAGAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     361 ATGAACACTACGGATACTTATGTGAGCTGGCCTTGCAGACTCAAAATTGGTGCCAAGAGC 420


4824R-5.IR_full       421 AAGAAGGATCCCCATGTGCGAATTGTGGGAAAGGTGGATCAGGTTCAGCGGGCAAAGGAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4824R-5.in silico     421 AAGAAGGATCCCCATGTGCGAATTGTGGGAAAGGTGGATCAGGTTCAGCGGGCAAAGGAG 480


4824R-5.IR_full       481 CGCATCCTTAGCAGTCTTGAC 501
                          |||||||||||||||||||||
4824R-5.in silico     481 CGCATCCTTAGCAGTCTTGAC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_057517.2  CG4824-RA, transcript variant A (BicC), mRNA 
100   482  0  0  0  NM_165144.1  CG4824-RB, transcript variant B (BicC), mRNA 
26.14   126  2  1  2  NM_165145.1  CG4824-RD, transcript variant D (BicC), mRNA 
0.2   1  3  4  2  NM_165234.1  CG31742-RA (CG31742), mRNA 
0   0  3  3  5  NM_142190.2  CG4931-RA (Sra-1), mRNA 
0   0  2  2  4  NM_142242.2  CG9597-RA (CG9597), mRNA 
0   0  2  2  4  NM_132108.2  CG3977-RA (Ctr1A), mRNA 
0   0  1  3  5  NM_136135.2  CG10132-RA (CG10132), mRNA 
0   0  1  3  2  NM_001042799.1  CG10966-RB, transcript variant B (rdgA), mRNA 
0   0  1  3  2  NM_078537.2  CG10966-RA, transcript variant A (rdgA), mRNA 
0   0  1  2  6  NM_139732.2  CG10542-RA (CG10542), mRNA 
0   0  1  2  5  NM_135735.1  CG5525-RA (CG5525), mRNA 
0   0  1  2  2  NM_136581.2  CG8237-RA (CG8237), mRNA 
0   0  0  10  7  NM_142599.1  CG4836-RC, transcript variant C (CG4836), mRNA 
0   0  0  10  7  NM_169882.1  CG4836-RB, transcript variant B (CG4836), mRNA 
0   0  0  10  7  NM_169883.1  CG4836-RA, transcript variant A (CG4836), mRNA 
0   0  0  7  7  NM_080259.1  CG14066-RA, transcript variant A (larp), mRNA 
0   0  0  7  7  NM_170366.1  CG14066-RB, transcript variant B (larp), mRNA 
0   0  0  7  7  NM_170365.1  CG14066-RC, transcript variant C (larp), mRNA 
0   0  0  6  5  NM_142975.2  CG5669-RA (CG5669), mRNA 
0   0  0  5  6  NM_168440.2  CG32072-RA (CG32072), mRNA 
0   0  0  5  3  NM_134989.2  CG32072-RA (CG32072), mRNA, ubiquitously expressed CG15437-RA (morgue), mRNA 
0   0  0  5  3  NM_078705.2  CG1685-RA (pen), mRNA 
0   0  0  4  9  NM_057507.3  CG8442-RA (Glu-RI), mRNA 
0   0  0  4  6  NM_137212.2  CG30089-RA (CG30089), mRNA 
0   0  0  4  5  NM_079205.3  CG7507-RA, transcript variant A (Dhc64C), mRNA 
0   0  0  4  4  NM_143422.1  CG11898-RA (CG11898), mRNA 
0   0  0  4  3  NM_079757.3  CG5610-RA (nAcRalpha-96Aa), mRNA 
0   0  0  4  3  NM_079747.2  CG5394-RA, transcript variant A (Aats-glupro), mRNA 
0   0  0  3  7  NM_140174.4  CG7839-RA (CG7839), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.