|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4821R-1 |
| Symbol |
Tequila |
Full Name |
Tequila |
| CG No |
CG4821 |
Old CG No |
CG4821 |
| Synonyms |
CG4948, CG18403, graal, SP72, CG18153, CK00198, anon-EST:ParkEST161, CG4821, BEST:GH18038, BEST:CK00198, Tequila, Teq, teq |
| Accession No (Link to NCBI) |
NM_140031.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13
[
PubMed ID = 16631589
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTATTGGTGCCTCCGCCTTT |
| Primer Seq. 3' |
aatctagaggtaccCAGGCGCATGCCAATTGGTTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTATTGGTG CCTCCGCCTT TCGACCATAA GTTTTATAGT CCTCAGTCGA CAAATCCAAG
0061 CATTAATCGA AATCAATCTG CATCTGGTCC CAACTCCCAA GCGGCAATTA AAGAAGCTCT
0121 TAAGCTTATG CTGCGTCCCT ATTTCAATCA CAGTGGGAAT GCCCAAGAGC AACTTGCCCA
0181 ACAGGCTGAA TCGGCTATAG TGTCTGTGAT TAGTAAAGGC GGTCAACCCA CTACCACCCA
0241 CCACACCAAG GCCAACTTTC CAAGACACCT AAAACAGACC CNTGACTTTT GACGCCGAAC
0301 TTATTAAAAG CGGGTGAACA GGAAAGCTTG GATTCCGTTG ACGACGACTA TGTTTTTCCA
0361 GATGCCAGGG AGACGTCCCG AACCGAGCAG ACCCTAGATC CCAGTACCAC ATACGCCTCG
0421 ACAAACTTTC AACGGAGCAC TCGCAGGGCT GAGTTAGATC CTGACACCCT AACCGCAACT
0481 ACAATGAAAA CCAATTGGCA TGcGCCTG |
| in silico PCR Fragment |
0001 TGTATTGGTG CCTCCGCCTT TCGACCATAA GTTTTATAGT CCTCAGTCGA CAAATCCAAG
0061 CATTAATCGA AATCAATCTG CATCTGGTCC CAACTCCCAA GCGGCAATTA AAGAAGCTCT
0121 TAAGCTTATG CTGCGTCCCT ATTTCAATCA CAGTGGGAAT GCCCAAGAGC AACTTGCTCA
0181 ACAGGCTGAA TCGGCTATAG TGTCTGTAAT TAGTAA--GC CGTCAA-CCA CTACCA-CCA
0241 CCACACCAAG GCCAAC-TTC CAAGACACCT AAAACAGACC C-TGAC-TTT GACGCCGAAC
0301 TTATT-AAAG CGGGTGAACA GGAAAGCTTG GATTCCGTTG ACGACGACTA TGTTTTTCCA
0361 GATGCCAGGG AGACGTCCCG AACCGAGCAG ACCCTAGATC CCAGTACCAC ATACGCCTCG
0421 ACAAACTTTC AACGGAGCAC TCGCAGGGCT GAGTTAGATC CTGACACCCT AACCGCAACT
0481 ACAATGAAAA CCAATTGGCA TGCGCCTG |
| Assemble Data |
4821R-1.IR_full 1 TGTATTGGTGCCTCCGCCTTTCGACCATAAGTTTTATAGTCCTCAGTCGACAAATCCAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4821R-1.in silico 1 TGTATTGGTGCCTCCGCCTTTCGACCATAAGTTTTATAGTCCTCAGTCGACAAATCCAAG 60
4821R-1.IR_full 61 CATTAATCGAAATCAATCTGCATCTGGTCCCAACTCCCAAGCGGCAATTAAAGAAGCTCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4821R-1.in silico 61 CATTAATCGAAATCAATCTGCATCTGGTCCCAACTCCCAAGCGGCAATTAAAGAAGCTCT 120
4821R-1.IR_full 121 TAAGCTTATGCTGCGTCCCTATTTCAATCACAGTGGGAATGCCCAAGAGCAACTTGCCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
4821R-1.in silico 121 TAAGCTTATGCTGCGTCCCTATTTCAATCACAGTGGGAATGCCCAAGAGCAACTTGCTCA 180
4821R-1.IR_full 181 ACAGGCTGAATCGGCTATAGTGTCTGTGATTAGTAAAGGCGGTCAACCCACTACCACCCA 240
||||||||||||||||||||||||||| |||||||| || ||||| ||||||||| |||
4821R-1.in silico 181 ACAGGCTGAATCGGCTATAGTGTCTGTAATTAGTAA--GCCGTCAA-CCACTACCA-CCA 240
4821R-1.IR_full 241 CCACACCAAGGCCAACTTTCCAAGACACCTAAAACAGACCCNTGACTTTTGACGCCGAAC 300
|||||||||||||||| |||||||||||||||||||||||| |||| |||||||||||||
4821R-1.in silico 241 CCACACCAAGGCCAAC-TTCCAAGACACCTAAAACAGACCC-TGAC-TTTGACGCCGAAC 300
4821R-1.IR_full 301 TTATTAAAAGCGGGTGAACAGGAAAGCTTGGATTCCGTTGACGACGACTATGTTTTTCCA 360
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
4821R-1.in silico 301 TTATT-AAAGCGGGTGAACAGGAAAGCTTGGATTCCGTTGACGACGACTATGTTTTTCCA 360
4821R-1.IR_full 361 GATGCCAGGGAGACGTCCCGAACCGAGCAGACCCTAGATCCCAGTACCACATACGCCTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4821R-1.in silico 361 GATGCCAGGGAGACGTCCCGAACCGAGCAGACCCTAGATCCCAGTACCACATACGCCTCG 420
4821R-1.IR_full 421 ACAAACTTTCAACGGAGCACTCGCAGGGCTGAGTTAGATCCTGACACCCTAACCGCAACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4821R-1.in silico 421 ACAAACTTTCAACGGAGCACTCGCAGGGCTGAGTTAGATCCTGACACCCTAACCGCAACT 480
4821R-1.IR_full 481 ACAATGAAAACCAATTGGCATGCGCCTG 508
||||||||||||||||||||||||||||
4821R-1.in silico 481 ACAATGAAAACCAATTGGCATGCGCCTG 508
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_168314.1 |
CG4821-RD, transcript variant D (Tequila), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_140031.1 |
CG4821-RA, transcript variant A (Tequila), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_168316.1 |
CG4821-RB, transcript variant B (Tequila), mRNA |
| 0.41 |
2 |
5 |
3 |
16 |
NM_136593.1 |
CG8181-RA (CG8181), mRNA |
| 0 |
0 |
11 |
39 |
312 |
NM_170227.2 |
CG31439-RA (CG31439), mRNA |
| 0 |
0 |
5 |
5 |
3 |
NM_078879.2 |
CG10363-RA (TepIV), mRNA |
| 0 |
0 |
5 |
3 |
2 |
NM_057907.2 |
CG4007-RA (Nrk), mRNA |
| 0 |
0 |
3 |
3 |
7 |
NM_139408.1 |
CG12023-RA, transcript variant A (GV1), mRNA |
| 0 |
0 |
2 |
6 |
9 |
NM_130498.1 |
CG13362-RA (CG13362), mRNA |
| 0 |
0 |
2 |
4 |
12 |
NM_136423.1 |
CG11101-RA (pwn), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_168120.1 |
CG10625-RB, transcript variant B (CG10625), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_168119.1 |
CG10625-RD, transcript variant D (CG10625), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_168121.1 |
CG10625-RC, transcript variant C (CG10625), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_139713.2 |
CG10625-RA, transcript variant A (CG10625), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_164582.1 |
CG3054-RA, transcript variant A (l(2)k05819), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_134985.2 |
CG3054-RB, transcript variant B (l(2)k05819), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079496.2 |
CG14637-RA (abs), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_135589.2 |
CG17104-RA (CG17104), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078517.2 |
CG2175-RA, transcript variant A (dec-1), mRNA |
| 0 |
0 |
1 |
7 |
18 |
NM_137883.1 |
CG13535-RA (CG13535), mRNA |
| 0 |
0 |
1 |
6 |
17 |
NM_142330.1 |
CG5225-RA (CG5225), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_167438.1 |
CG9151-RB, transcript variant B (acj6), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_001014744.1 |
CG9151-RC, transcript variant C (acj6), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_080137.2 |
CG9151-RA, transcript variant A (acj6), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_001014743.1 |
CG9151-RD, transcript variant D (acj6), mRNA |
| 0 |
0 |
1 |
5 |
15 |
NM_167212.1 |
CG32694-RB, transcript variant B (CG32694), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167193.1 |
CG32702-RA (CG32702), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_130685.1 |
CG14420-RA (CG14420), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166259.1 |
CG30325-RA (CG30325), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_132357.3 |
CG1468-RA (CG1468), mRNA |
|
|
|
