|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4788R-3 |
| Symbol |
CG4788 |
Full Name |
CG4788 |
| CG No |
CG4788 |
Old CG No |
CG4788 |
| Synonyms |
CG4788 |
| Accession No (Link to NCBI) |
NM_135655.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTGGAGAAGACGAAGAGGA |
| Primer Seq. 3' |
aatctagaggtaccTGGTACTGCACCCTCTACGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTGGAGAA GACGAAGAGG AAGTGCTNCC CAGTTCGTGT CCGCGCCGTA CATAATGCCC
0061 AAGCCACTGG TAGTCAATGG GCACACATCG ATCTTTCAAG TGGGCGGCGA AATGGCCCAT
0121 ATGGGCGACA TTCCGGCGCT CTCGAAAATT AGTGATGGTG GACAGCTAGT ATACCGAATA
0181 AAGCCGGGCA CCCATGCCCA TGTCATCAAC TATGTGATCA TCGACGAGGG CTCCGACNTC
0241 GCTGGAGAAG GCCAGATCCG GCGACCAGGA GGCCATGCGT CTGCTGCTGG AATCGCAACG
0301 TGACAGTGCT GCGTCCAAGC TACAAAAGTT TTTAACCAAC CATAAGGGAA CTCCGCCATC
0361 GGGCTATATT TCATCGCTAA TACTATCACg gCCAGTGGCC AGCGTCTCAA CGTCTGCGGG
0421 CACTCCAgcN GCCACTGGCC ATGTGACTAC TCCTCAAATC CATCCGAACT TCTCCATCGC
0481 CAGCGTAGAG GGTGCAGTAC CA |
| in silico PCR Fragment |
0001 TGGTGGAGAA GACGAAGAGG AAGTGCT-CC CAGTTCGTGT CCGCGCCGTA CATAATGCCC
0061 AAGCCACTGG TAGTCAATGG GCACACATCG ATCTTTCAAG TGGGCGGCGA AATGGCCCAT
0121 ATGGGCGACA TTCCGGCGCT CTCGAAAATT AGTGATGGTG GACAGCTAGT ATACCGAATA
0181 AAGCCGGGCA CCCATGCCCA TGTCATCAAC TATGTGATCA TCGACGAGGG CTCCGAC-TC
0241 GCTGGAGAAG GCCAGATCCG GCGACCAGGA GGCCATGCGT CTGCTGCTGG AATCGCAACG
0301 TGACAGTGCT GCGTCCAAGC TACAAAAGTT TTTAACCAAC CATAAGGGAA CTCCGCCATC
0361 GGGCTATATT TCATCGCTAA TACTATCACG GCCAGTGGCC AGCGTCTCAA CGTCTGCGGG
0421 CACTCCAGCG GCCACTGGCC ATGTGACTAC TCCTCAAATC CATCCGAACT TCTCCATCGC
0481 CAGCGTAGAG GGTGCAGTAC CA |
| Assemble Data |
4788R-3.IR_full 1 TGGTGGAGAAGACGAAGAGGAAGTGCTNCCCAGTTCGTGTCCGCGCCGTACATAATGCCC 60
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
4788R-3.in silico 1 TGGTGGAGAAGACGAAGAGGAAGTGCT-CCCAGTTCGTGTCCGCGCCGTACATAATGCCC 60
4788R-3.IR_full 61 AAGCCACTGGTAGTCAATGGGCACACATCGATCTTTCAAGTGGGCGGCGAAATGGCCCAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 61 AAGCCACTGGTAGTCAATGGGCACACATCGATCTTTCAAGTGGGCGGCGAAATGGCCCAT 120
4788R-3.IR_full 121 ATGGGCGACATTCCGGCGCTCTCGAAAATTAGTGATGGTGGACAGCTAGTATACCGAATA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 121 ATGGGCGACATTCCGGCGCTCTCGAAAATTAGTGATGGTGGACAGCTAGTATACCGAATA 180
4788R-3.IR_full 181 AAGCCGGGCACCCATGCCCATGTCATCAACTATGTGATCATCGACGAGGGCTCCGACNTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
4788R-3.in silico 181 AAGCCGGGCACCCATGCCCATGTCATCAACTATGTGATCATCGACGAGGGCTCCGAC-TC 240
4788R-3.IR_full 241 GCTGGAGAAGGCCAGATCCGGCGACCAGGAGGCCATGCGTCTGCTGCTGGAATCGCAACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 241 GCTGGAGAAGGCCAGATCCGGCGACCAGGAGGCCATGCGTCTGCTGCTGGAATCGCAACG 300
4788R-3.IR_full 301 TGACAGTGCTGCGTCCAAGCTACAAAAGTTTTTAACCAACCATAAGGGAACTCCGCCATC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 301 TGACAGTGCTGCGTCCAAGCTACAAAAGTTTTTAACCAACCATAAGGGAACTCCGCCATC 360
4788R-3.IR_full 361 GGGCTATATTTCATCGCTAATACTATCACGGCCAGTGGCCAGCGTCTCAACGTCTGCGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 361 GGGCTATATTTCATCGCTAATACTATCACGGCCAGTGGCCAGCGTCTCAACGTCTGCGGG 420
4788R-3.IR_full 421 CACTCCAGCNGCCACTGGCCATGTGACTACTCCTCAAATCCATCCGAACTTCTCCATCGC 480
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
4788R-3.in silico 421 CACTCCAGCGGCCACTGGCCATGTGACTACTCCTCAAATCCATCCGAACTTCTCCATCGC 480
4788R-3.IR_full 481 CAGCGTAGAGGGTGCAGTACCA 502
||||||||||||||||||||||
4788R-3.in silico 481 CAGCGTAGAGGGTGCAGTACCA 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135655.2 |
CG4788-RA (CG4788), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_166315.1 |
CG12758-RB, transcript variant B (sano), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_166314.1 |
CG12758-RA, transcript variant A (sano), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057598.3 |
CG10739-RA (pigeon), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168939.1 |
CG32448-RA (CG32448), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168111.3 |
CG32423-RA, transcript variant A (alan-shepard), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168113.2 |
CG32423-RD, transcript variant D (alan-shepard), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168112.1 |
CG32423-RB, transcript variant B (alan-shepard), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079543.2 |
CG1257-RA (alpha-Est3), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_138021.2 |
CG3860-RA (CG3860), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165836.1 |
CG7776-RB, transcript variant B (E(Pc)), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078974.2 |
CG7776-RA, transcript variant A (E(Pc)), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169487.1 |
CG31342-RA (CG31342), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139802.2 |
CG32392-RB, transcript variant B (CG32392), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_166242.1 |
CG10936-RB, transcript variant B (CG10936), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137395.1 |
CG10936-RA, transcript variant A (CG10936), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142593.1 |
CG4459-RA (CG4459), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168049.1 |
CG32257-RA (Gr64b), mRNA |
| 0 |
0 |
0 |
7 |
15 |
NM_167482.3 |
CG32580-RA (CG32580), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_057380.2 |
CG8355-RA, transcript variant A (sli), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_057381.2 |
CG8355-RB, transcript variant B (sli), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_057379.2 |
CG8355-RC, transcript variant C (sli), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_144447.1 |
CG18870-RA (CG18870), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_078722.2 |
CG17941-RA (ds), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176184.2 |
CG18250-RC, transcript variant C (Dg), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_166791.1 |
CG1793-RB, transcript variant B (MED26), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_134789.1 |
CG15356-RA (CG15356), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
|
|
|
|
