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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4774R-4 
 Symbol CG4774  Full Name CG4774 
 CG No CG4774  Old CG No CG4774 
 Synonyms CG4774 
 Accession No (Link to NCBI) NM_143161.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACGTTTTGGGAGTCGGCGGAA 
 Primer Seq. 3' 
aatctagaggtaccGGGGTCCAGGAAGGAACCGAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACGTTTTGGG AGTCGGCGGA AGCAGCTTCG TGAACTGCCT GAATCGCTAT GCGGCCGCCA 
0061 CGGGATTCAT TCGGATCTCG TTTCTGGACA TTAAGCGGCG CAGGAACTAC GAGTTAGCCA 
0121 GACTTCGCCT CTATGCCGAT GAGAAGAAGC AGTCGCTCCA TTTGCGCACC TTGCAGGGAC 
0181 GCCATCTCCT GCAGGGTGTC ATCGAAAGGA AGAACTTCCT GGTGGATGAC ATCCGGGAGG 
0241 CCAGGCACAA AGTCCAGGAG CGGGTGCGCG ANGAAGATCG ACGAGATACG CGAAGAGCGA 
0301 GAGAACATTA TGACCATACC CAACATGCTG ACCATCAGTC GTGCCGTCCT CTCACCCTAC 
0361 ATCGGATATG TTATAGTCCA GGGCGATTTC ACCCTGGGCA TGAGCCTTCT AGCGTTCGCC 
0421 GGCATCACAG ATCTGTTGGA TGGACAGATC GCACGACGGT GGCCTTCGCA GGCGAGCAAG 
0481 TTCGGTTCCT TCCTGGACCC C 
 in silico PCR Fragment 
0001 ACGTTTTGGG AGTCGGCGGA AGCAGCTTCG TGAACTGCCT GAATCGCTAT GCGGCCGCCA 
0061 CGGGATTCAT TCGGATCTCG TTTCTGGACA TTAAGCGGCG CAGGAACTAC GAGTTAGCCA 
0121 GACTTCGCCT CTATGCCGAT GAGAAGAAGC AGTCGCTCCA TTTGCGCACC TTGCAGGGAC 
0181 GCCATCTCCT GCAGGGTGTC ATCGAAAGGA AGAACTTCCT GGTGGATGAC ATCCGGGAGG 
0241 CCAGGCACAA AGTCCAGGAG CGGGTGCGCG A-GAAGATCG ACGAGATACG CGAAGAGCGA 
0301 GAGAACATTA TGACCATACC CAACATGCTG ACCATCAGTC GTGCCGTCCT CTCACCCTAC 
0361 ATCGGATATG TTATAGTCCA GGGCGATTTC ACCCTGGGCA TGAGCCTTCT AGCGTTCGCC 
0421 GGCATCACAG ATCTGTTGGA TGGACAGATC GCACGACGGT GGCCTTCGCA GGCGAGCAAG 
0481 TTCGGTTCCT TCCTGGACCC C 
 Assemble Data 

4774R-4.IR_full       1   ACGTTTTGGGAGTCGGCGGAAGCAGCTTCGTGAACTGCCTGAATCGCTATGCGGCCGCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     1   ACGTTTTGGGAGTCGGCGGAAGCAGCTTCGTGAACTGCCTGAATCGCTATGCGGCCGCCA 60


4774R-4.IR_full       61  CGGGATTCATTCGGATCTCGTTTCTGGACATTAAGCGGCGCAGGAACTACGAGTTAGCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     61  CGGGATTCATTCGGATCTCGTTTCTGGACATTAAGCGGCGCAGGAACTACGAGTTAGCCA 120


4774R-4.IR_full       121 GACTTCGCCTCTATGCCGATGAGAAGAAGCAGTCGCTCCATTTGCGCACCTTGCAGGGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     121 GACTTCGCCTCTATGCCGATGAGAAGAAGCAGTCGCTCCATTTGCGCACCTTGCAGGGAC 180


4774R-4.IR_full       181 GCCATCTCCTGCAGGGTGTCATCGAAAGGAAGAACTTCCTGGTGGATGACATCCGGGAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     181 GCCATCTCCTGCAGGGTGTCATCGAAAGGAAGAACTTCCTGGTGGATGACATCCGGGAGG 240


4774R-4.IR_full       241 CCAGGCACAAAGTCCAGGAGCGGGTGCGCGANGAAGATCGACGAGATACGCGAAGAGCGA 300
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
4774R-4.in silico     241 CCAGGCACAAAGTCCAGGAGCGGGTGCGCGA-GAAGATCGACGAGATACGCGAAGAGCGA 300


4774R-4.IR_full       301 GAGAACATTATGACCATACCCAACATGCTGACCATCAGTCGTGCCGTCCTCTCACCCTAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     301 GAGAACATTATGACCATACCCAACATGCTGACCATCAGTCGTGCCGTCCTCTCACCCTAC 360


4774R-4.IR_full       361 ATCGGATATGTTATAGTCCAGGGCGATTTCACCCTGGGCATGAGCCTTCTAGCGTTCGCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     361 ATCGGATATGTTATAGTCCAGGGCGATTTCACCCTGGGCATGAGCCTTCTAGCGTTCGCC 420


4774R-4.IR_full       421 GGCATCACAGATCTGTTGGATGGACAGATCGCACGACGGTGGCCTTCGCAGGCGAGCAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4774R-4.in silico     421 GGCATCACAGATCTGTTGGATGGACAGATCGCACGACGGTGGCCTTCGCAGGCGAGCAAG 480


4774R-4.IR_full       481 TTCGGTTCCTTCCTGGACCCC 501
                          |||||||||||||||||||||
4774R-4.in silico     481 TTCGGTTCCTTCCTGGACCCC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_170237.1  CG4774-RB, transcript variant B (CG4774), mRNA 
100   482  0  0  0  NM_143161.1  CG4774-RA, transcript variant A (CG4774), mRNA 
100   482  0  0  0  NM_170238.1  CG4774-RC, transcript variant C (CG4774), mRNA 
0.41   2  2  3  2  NM_130653.2  CG13759-RA (CG13759), mRNA 
0.2   1  2  2  6  NM_132037.2  CG12239-RA (CG12239), mRNA 
0   0  4  2  6  NM_167940.1  CG32305-RA (CG32305), mRNA 
0   0  2  3  8  NM_169545.1  CG9374-RF, transcript variant F (lkb1), mRNA 
0   0  2  3  8  NM_169547.1  CG9374-RI, transcript variant I (lkb1), mRNA 
0   0  2  3  8  NM_169542.1  CG9374-RC, transcript variant C (lkb1), mRNA 
0   0  2  3  8  NM_169546.1  CG9374-RH, transcript variant H (lkb1), mRNA 
0   0  2  3  8  NM_169541.1  CG9374-RA, transcript variant A (lkb1), mRNA 
0   0  2  3  8  NM_169544.1  CG9374-RE, transcript variant E (lkb1), mRNA 
0   0  2  3  8  NM_169543.1  CG9374-RD, transcript variant D (lkb1), mRNA 
0   0  2  3  8  NM_142045.2  CG9374-RB, transcript variant B (lkb1), mRNA 
0   0  2  3  3  NM_169486.1  CG7381-RC, transcript variant C (CG7381), mRNA 
0   0  2  3  3  NM_169485.1  CG7381-RB, transcript variant B (CG7381), mRNA 
0   0  2  3  3  NM_141985.1  CG7381-RA, transcript variant A (CG7381), mRNA 
0   0  1  3  5  NM_080142.2  CG1925-RA (mus205), mRNA 
0   0  1  3  3  NM_138192.1  CG13891-RA (CG13891), mRNA 
0   0  1  2  7  NM_080247.2  CG13221-RA (Vhl), mRNA 
0   0  1  2  4  NM_140569.2  CG5841-RA (mib1), mRNA 
0   0  1  2  3  NM_141447.2  CG14609-RA (CG14609), mRNA 
0   0  1  2  2  NM_135388.2  CG9233-RA (fu2), mRNA 
0   0  0  10  12  NM_140627.2  CG4784-RA (CG4784), mRNA 
0   0  0  5  13  NM_001043070.1  CG34146-RA (brp), mRNA 
0   0  0  5  6  NM_140914.2  CG7749-RA, transcript variant A (fat2), mRNA 
0   0  0  5  6  NM_001031967.1  CG7749-RB, transcript variant B (fat2), mRNA 
0   0  0  5  3  NM_057512.3  CG3724-RA (Pgd), mRNA 
0   0  0  4  8  NM_169197.2  CG31187-RA (CG31187), mRNA 
0   0  0  4  5  NM_167939.1  CG32301-RA (CG32301), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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