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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4768R-3 |
| Symbol |
CG4768 |
Full Name |
CG4768 |
| CG No |
CG4768 |
Old CG No |
CG4768 |
| Synonyms |
CG4768 |
| Accession No (Link to NCBI) |
NM_132936.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTATTTGGCCGGAAAGCCACT |
| Primer Seq. 3' |
aatctagaggtaccTACATGAAGCGCTGGCGTTTA |
| Predicted Fragment Size |
408 |
| IR fragment full Seq |
0001 CTATTTGGCC GGAAAGCCAC TACCGCCATG CAACTCAAAT CCACATGCCA ATCGTCGTTC
0061 GCCGAANGCC GGAGCATCAC CACCAGAGTG GCAGTGGTGG CTCTGGCCTG GGATTCCACG
0121 GCTCCGGATT GGGCCTGACC GGTGGTCCGC TCACGGCTGC CGGGGGTGGG CACTTGCTGT
0181 CCGCCGGAGC AGCCGGTGGC CGTCAGGAGG GAACGGGCGG TGcCCCCGGC GGCGGNAATC
0241 GGCGATGACT TGCACACGTT CAAGGAGGCC TTGGTTTTGC GGCCACGTGG CCCGGAACTA
0301 TTGGCATTTG TCGCCAACGA AATCGCGCGA CATTGCAAGC GTCCCGGGAG TCCCATTGAC
0361 GACAAAATGG ACATTTGCCA CTACAGTAGT AANACGCCAG CGCTTCATGT A |
| in silico PCR Fragment |
0001 CTATTTGGCC GGAAAGCCAC TACCGCCATG CAACTCAAAT CCACATGCCA ATCGTCGTTC
0061 GCCGAA-GCC GGAGCATCAC CACCAGAGTG GCAGTGGTGG CTCTGGCCTG GGATTCCACG
0121 GCTCCGGATT GGGCCTGACC GGTGGTCCGC TCACGGCTGC CGGGGGTGGG CACTTGCTGT
0181 CCGCCGGAGC AGCCGGTGGC CGTCAGGAGG GAACGGGCGG TGCCCCCGGC GGCGG-AATC
0241 GGCGATGACT TGCACACGTT CAAGGAGGCC TTGGTTTTGC GGCCACGTGG CCCGGAACTA
0301 TTGGCATTTG TCGCCAACGA AATCGCGCGA CATTGCAAGC GTCCCGGGAG TCCCATTGAC
0361 GACAAAATGG ACATTTGCCA CTACAGTAGT AA-ACGCCAG CGCTTCATGT A |
| Assemble Data |
4768R-3.IR_full 1 CTATTTGGCCGGAAAGCCACTACCGCCATGCAACTCAAATCCACATGCCAATCGTCGTTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4768R-3.in silico 1 CTATTTGGCCGGAAAGCCACTACCGCCATGCAACTCAAATCCACATGCCAATCGTCGTTC 60
4768R-3.IR_full 61 GCCGAANGCCGGAGCATCACCACCAGAGTGGCAGTGGTGGCTCTGGCCTGGGATTCCACG 120
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
4768R-3.in silico 61 GCCGAA-GCCGGAGCATCACCACCAGAGTGGCAGTGGTGGCTCTGGCCTGGGATTCCACG 120
4768R-3.IR_full 121 GCTCCGGATTGGGCCTGACCGGTGGTCCGCTCACGGCTGCCGGGGGTGGGCACTTGCTGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4768R-3.in silico 121 GCTCCGGATTGGGCCTGACCGGTGGTCCGCTCACGGCTGCCGGGGGTGGGCACTTGCTGT 180
4768R-3.IR_full 181 CCGCCGGAGCAGCCGGTGGCCGTCAGGAGGGAACGGGCGGTGCCCCCGGCGGCGGNAATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
4768R-3.in silico 181 CCGCCGGAGCAGCCGGTGGCCGTCAGGAGGGAACGGGCGGTGCCCCCGGCGGCGG-AATC 240
4768R-3.IR_full 241 GGCGATGACTTGCACACGTTCAAGGAGGCCTTGGTTTTGCGGCCACGTGGCCCGGAACTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4768R-3.in silico 241 GGCGATGACTTGCACACGTTCAAGGAGGCCTTGGTTTTGCGGCCACGTGGCCCGGAACTA 300
4768R-3.IR_full 301 TTGGCATTTGTCGCCAACGAAATCGCGCGACATTGCAAGCGTCCCGGGAGTCCCATTGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4768R-3.in silico 301 TTGGCATTTGTCGCCAACGAAATCGCGCGACATTGCAAGCGTCCCGGGAGTCCCATTGAC 360
4768R-3.IR_full 361 GACAAAATGGACATTTGCCACTACAGTAGTAANACGCCAGCGCTTCATGTA 411
|||||||||||||||||||||||||||||||| ||||||||||||||||||
4768R-3.in silico 361 GACAAAATGGACATTTGCCACTACAGTAGTAA-ACGCCAGCGCTTCATGTA 411
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
390 |
0 |
0 |
0 |
NM_132936.2 |
CG4768-RA (CG4768), mRNA |
| 0 |
0 |
3 |
2 |
14 |
NM_133075.2 |
CG6461-RA (CG6461), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_205883.1 |
CG4574-RD, transcript variant D (Plc21C), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_057503.4 |
CG4574-RB, transcript variant B (Plc21C), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079453.2 |
CG6975-RA (gig), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_132581.1 |
CG11138-RC, transcript variant C (CG11138), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136866.2 |
CG8983-RB, transcript variant B (ERp60), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165849.1 |
CG8983-RA, transcript variant A (ERp60), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_130671.2 |
CG2713-RA (CG2713), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079418.2 |
CG6895-RA (GNBP1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079633.2 |
CG6476-RA, transcript variant A (Su(var)3-9), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142774.2 |
CG18596-RA (CG18596), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143497.2 |
CG7896-RA (CG7896), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132468.2 |
CG1394-RA (CG1394), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137558.2 |
CG11906-RA (CG11906), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001031909.1 |
CG33517-RE, transcript variant E (D2R), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014760.2 |
CG33517-RA, transcript variant A (D2R), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014757.2 |
CG33517-RD, transcript variant D (D2R), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014758.2 |
CG33517-RC, transcript variant C (D2R), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167218.1 |
CG32685-RC (CG32685), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080142.2 |
CG1925-RA (mus205), mRNA |
| 0 |
0 |
0 |
9 |
18 |
NM_057849.3 |
CG3733-RA (Chd1), mRNA |
| 0 |
0 |
0 |
8 |
6 |
NM_136690.2 |
CG1513-RA (CG1513), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_136891.2 |
CG8877-RA (prp8), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_170625.1 |
CG17034-RC, transcript variant C (CG17034), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_170624.1 |
CG17034-RB, transcript variant B (CG17034), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_080707.2 |
CG3430-RA (CG3430), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_135244.2 |
CG9200-RA (CG9200), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_143196.1 |
CG8968-RA (CG8968), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
 |
| + |
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