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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4724R-3 
 Symbol CG32568  Full Name CG32568 
 CG No CG32568  Old CG No CG4724 
 Synonyms CG4724, CG32568 
 Accession No (Link to NCBI) NM_167542.2 
 Inserted Chr. lll 
 Insertional Mutation  2 lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAAAAGCGACCAACCTGTCGGA 
 Primer Seq. 3' 
aatctagaggtaccAGAGCCGTAGAAGACCTTTGA 
 Predicted Fragment Size 
491 
 IR fragment full Seq 
0001 AAAAGCGACC AACCTGTCGG ATAAGAAGGC AATCGTAGAT CTATTCTCTA GTTTCGTGAA 
0061 TACACATCAT TTTCGACCCA ACCCCGATGG TCAGAGGATT GATAAAATAA TTGTAATAAA 
0121 GTTANGTGGA ACGCTTAGAA ACTACAGATG TAAATGATCG CAAATTTATG CAGAGTATAT 
0181 TGCGGCGCGT CTATTTAAAN GTGCACCGAC TTGCGTCTAT TTATTCGAAA TCAGCTCAAC 
0241 GATGTATTCT TTCGATTGAT TTTCGAAGGA ACTGACTTTC ATTGCATCCC AGAAATTCTG 
0301 GAAATTTACT ATGATATAAT CAAGGGATTT ACTCAACCAT ATGAGACTGA ACATGAGCAA 
0361 CTGCTCTTTA AGATCCTTTT GCCTTTGCAC AAGCCGCCAT CGTTAACAAA GTATTTTCAT 
0421 CAGCTGATAA AATGCATTAT TGCATTCCTT AACCAATATC CATCTTTCAT TGAAAAATAT 
0481 GTCAAAGGTC TTCTACGGCT CT 
 in silico PCR Fragment 
0001 AAAAGCGACC AACCTGTCGG ATAAGAAGGC AATCGTAGAT CTATTCTCTA GTTTCGTGAA 
0061 TACACATCAT TTTCGACCCA ACCCCGATGG TCAGAGGATT GATAAAATAA TT-------- 
0121 -TTA-GTGGA ACGCTTAGAA ACTACAGATG TAAATGATCG CAAATTTATG CAGAGTATAT 
0181 TGCGGCGCGT CTATTTAAA- GTGCACCGAC TTGCGTCTAT TTATTCGAAA TCAGCTCAAC 
0241 GATGTATTCT TTCGATTGAT TTTCGAAGGA ACTGACTTTC ATTGCATCCC AGAAATTCTG 
0301 GAAATTTACT ATGATATAAT CAAGGGATTT ACTCAACCAT ATGAGACTGA ACATGAGCAA 
0361 CTGCTCTTTA AGATCCTTTT GCCTTTGCAC AAGCCGCCAT CGTTAACAAA GTATTTTCAT 
0421 CAGCTGATAA AATGCATTAT TGCATTCCTT AACCAATATC CATCTTTCAT TGAAAAATAT 
0481 GTCAAAGGTC TTCTACGGCT CT 
 Assemble Data 

4724R-3.IR_full       1   AAAAGCGACCAACCTGTCGGATAAGAAGGCAATCGTAGATCTATTCTCTAGTTTCGTGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     1   AAAAGCGACCAACCTGTCGGATAAGAAGGCAATCGTAGATCTATTCTCTAGTTTCGTGAA 60


4724R-3.IR_full       61  TACACATCATTTTCGACCCAACCCCGATGGTCAGAGGATTGATAAAATAATTGTAATAAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||        
4724R-3.in silico     61  TACACATCATTTTCGACCCAACCCCGATGGTCAGAGGATTGATAAAATAATT-------- 120


4724R-3.IR_full       121 GTTANGTGGAACGCTTAGAAACTACAGATGTAAATGATCGCAAATTTATGCAGAGTATAT 180
                           ||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     121 -TTA-GTGGAACGCTTAGAAACTACAGATGTAAATGATCGCAAATTTATGCAGAGTATAT 180


4724R-3.IR_full       181 TGCGGCGCGTCTATTTAAANGTGCACCGACTTGCGTCTATTTATTCGAAATCAGCTCAAC 240
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     181 TGCGGCGCGTCTATTTAAA-GTGCACCGACTTGCGTCTATTTATTCGAAATCAGCTCAAC 240


4724R-3.IR_full       241 GATGTATTCTTTCGATTGATTTTCGAAGGAACTGACTTTCATTGCATCCCAGAAATTCTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     241 GATGTATTCTTTCGATTGATTTTCGAAGGAACTGACTTTCATTGCATCCCAGAAATTCTG 300


4724R-3.IR_full       301 GAAATTTACTATGATATAATCAAGGGATTTACTCAACCATATGAGACTGAACATGAGCAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     301 GAAATTTACTATGATATAATCAAGGGATTTACTCAACCATATGAGACTGAACATGAGCAA 360


4724R-3.IR_full       361 CTGCTCTTTAAGATCCTTTTGCCTTTGCACAAGCCGCCATCGTTAACAAAGTATTTTCAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     361 CTGCTCTTTAAGATCCTTTTGCCTTTGCACAAGCCGCCATCGTTAACAAAGTATTTTCAT 420


4724R-3.IR_full       421 CAGCTGATAAAATGCATTATTGCATTCCTTAACCAATATCCATCTTTCATTGAAAAATAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4724R-3.in silico     421 CAGCTGATAAAATGCATTATTGCATTCCTTAACCAATATCCATCTTTCATTGAAAAATAT 480


4724R-3.IR_full       481 GTCAAAGGTCTTCTACGGCTCT 502
                          ||||||||||||||||||||||
4724R-3.in silico     481 GTCAAAGGTCTTCTACGGCTCT 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   473  0  0  2  NM_167542.2  CG32568-RA (CG32568), mRNA 
0   0  3  4  4  NM_001015303.1  CG40113-PB.3 (CG40113), mRNA 
0   0  3  4  4  NM_001015302.1  CG40113-PA.3 (CG40113), mRNA 
0   0  2  3  3  NM_175977.1  CG14026-RB, transcript variant B (tkv), mRNA 
0   0  2  3  2  NM_175975.1  CG14026-RA, transcript variant A (tkv), mRNA 
0   0  2  3  2  NM_175976.1  CG14026-RC, transcript variant C (tkv), mRNA 
0   0  2  3  2  NM_175978.1  CG14026-RD, transcript variant D (tkv), mRNA 
0   0  1  7  2  NM_206593.1  CG1873-RC, transcript variant C (Ef1alpha100E), mRNA 
0   0  1  7  2  NM_170570.1  CG1873-RB, transcript variant B (Ef1alpha100E), mRNA 
0   0  1  7  2  NM_206592.1  CG1873-RD, transcript variant D (Ef1alpha100E), mRNA 
0   0  1  7  2  NM_079872.4  CG1873-RA, transcript variant A (Ef1alpha100E), mRNA 
0   0  1  4  2  NM_164401.3  CG31795-RB, transcript variant B (ia2), mRNA 
0   0  1  2  4  NM_001038756.1  CG33968-RA (CG33968), mRNA 
0   0  0  5  3  NM_142384.1  CG14325-RA (CG14325), mRNA 
0   0  0  4  5  NM_135643.2  CG6192-RA (CG6192), mRNA 
0   0  0  4  4  NM_137512.2  CG5469-RD, transcript variant D (Gint3), mRNA 
0   0  0  4  3  NM_138206.1  CG3371-RA (CG3371), mRNA 
0   0  0  4  3  NM_140523.2  CG7764-RA (Tfb2), mRNA 
0   0  0  3  8  NM_139573.1  CG1120-RA (CG1120), mRNA 
0   0  0  3  8  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
0   0  0  3  8  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
0   0  0  3  3  NM_058090.2  CG7121-RA (Tehao), mRNA 
0   0  0  3  3  NM_164990.2  CG6716-RB, transcript variant B (prd), mRNA 
0   0  0  3  2  NM_139922.2  CG13676-RA (CG13676), mRNA 
0   0  0  3  2  NM_001042866.1  CG34102-RA (BG642163), mRNA 
0   0  0  2  9  NM_079876.2  CG1483-RA, transcript variant A (Map205), mRNA 
0   0  0  2  9  NM_170586.1  CG1483-RB, transcript variant B (Map205), mRNA 
0   0  0  2  8  NM_135017.2  CG15628-RA (CG15628), mRNA 
0   0  0  2  7  NM_136338.2  CG12792-RA (l(2)09851), mRNA 
0   0  0  2  6  NM_078607.2  CG6157-RA (dah), mRNA 

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