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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4698R-2 |
| Symbol |
Wnt4 |
Full Name |
Wnt oncogene analog 4 |
| CG No |
CG4698 |
Old CG No |
CG4698 |
| Synonyms |
wnt4, Wnt-4, DWnt4, Dwnt4, DWnt-4, Wnt, wnt-4, clone 2.4, anon-EST:Liang-2.4, CG4698, Wnt4 |
| Accession No (Link to NCBI) |
NM_057624.2 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGGATTACCTGGGAATCTGG |
| Primer Seq. 3' |
aatctagaggtaccCTCCCTGTTGTGCTTTTTTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGGATTACC TGGGAATCTG GGATACGGCG GGACCATGCT GAACGGCGGA GGAGTTGGAG
0061 GTGCTGCCGG AATGGGCCTA GGAATCGGCT CCAACACTAA TAACATGGAT ATGCAGCAAG
0121 GCCTTTACAA CGAACACTTC ATTTCGGAGC ACACGGTGAT GGCGGTGTTC ACTTCGCAGG
0181 GCCAAGTGGG TGGACCCTGC AGATACATGC CTGCCACGCG GCGTCAGAAC CACCAGTGTC
0241 GCAAGGAGAC GGGATTGCCG GGAACCCTTA GCGAGGCCCG CCGTCTGGCC ACCACCCACT
0301 GCGAGGAACA GTTCCGCTAC GACCGCTGGA ACTGCTCGAT CGAGACGCGC GGAAAGCGGA
0361 ACATCTTCAA GAAGCTCTAC AAGGAGACTG CGCTTCGTCC NACGCGCTCA CAgCGGCCGC
0421 CATGACCCAC TCGATAGCTA GGGCCTGTGC CGAGGGACGT ATGACCAAGT GCAGCTGCGG
0481 GCCAAAAAAG CACAACAGGG AG |
| in silico PCR Fragment |
0001 AAGGATTACC TGGGAATCTG GGATACGGCG GGACCATGCT GAACGGCGGA GGAGTTGGAG
0061 GTGCTGCCGG AATGGGCCTA GGAATCGGCT CCAACACTAA TAACATGGAT ATGCAGCAAG
0121 GCCTTTACAA CGAACACTTC ATTTCGGAGC ACACGGTGAT GGCGGTGTTC ACTTCGCAGG
0181 GCCAAGTGGG TGGACCCTGC AGATACATGC CTGCCACGCG GCGTCAGAAC CACCAGTGTC
0241 GCAAGGAGAC GGGATTGCCG GGAACCCTTA GCGAGGCCCG CCGTCTGGCC ACCACCCACT
0301 GCGAGGAACA GTTCCGCTAC GACCGCTGGA ACTGCTCGAT CGAGACGCGC GGAAAGCGGA
0361 ACATCTTCAA GAAGCTCTAC AAGGAGACTG C-CTTCGTCC -ACGCGCTCA CAGCGGCCGC
0421 CATGACCCAC TCGATAGCCA GGGCCTGTGC CGAGGGACGT ATGACCAAGT GCAGCTGCGG
0481 GCCAAAAAAG CACAACAGGG AG |
| Assemble Data |
4698R-2.IR_full 1 AAGGATTACCTGGGAATCTGGGATACGGCGGGACCATGCTGAACGGCGGAGGAGTTGGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 1 AAGGATTACCTGGGAATCTGGGATACGGCGGGACCATGCTGAACGGCGGAGGAGTTGGAG 60
4698R-2.IR_full 61 GTGCTGCCGGAATGGGCCTAGGAATCGGCTCCAACACTAATAACATGGATATGCAGCAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 61 GTGCTGCCGGAATGGGCCTAGGAATCGGCTCCAACACTAATAACATGGATATGCAGCAAG 120
4698R-2.IR_full 121 GCCTTTACAACGAACACTTCATTTCGGAGCACACGGTGATGGCGGTGTTCACTTCGCAGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 121 GCCTTTACAACGAACACTTCATTTCGGAGCACACGGTGATGGCGGTGTTCACTTCGCAGG 180
4698R-2.IR_full 181 GCCAAGTGGGTGGACCCTGCAGATACATGCCTGCCACGCGGCGTCAGAACCACCAGTGTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 181 GCCAAGTGGGTGGACCCTGCAGATACATGCCTGCCACGCGGCGTCAGAACCACCAGTGTC 240
4698R-2.IR_full 241 GCAAGGAGACGGGATTGCCGGGAACCCTTAGCGAGGCCCGCCGTCTGGCCACCACCCACT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 241 GCAAGGAGACGGGATTGCCGGGAACCCTTAGCGAGGCCCGCCGTCTGGCCACCACCCACT 300
4698R-2.IR_full 301 GCGAGGAACAGTTCCGCTACGACCGCTGGAACTGCTCGATCGAGACGCGCGGAAAGCGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 301 GCGAGGAACAGTTCCGCTACGACCGCTGGAACTGCTCGATCGAGACGCGCGGAAAGCGGA 360
4698R-2.IR_full 361 ACATCTTCAAGAAGCTCTACAAGGAGACTGCGCTTCGTCCNACGCGCTCACAGCGGCCGC 420
||||||||||||||||||||||||||||||| |||||||| |||||||||||||||||||
4698R-2.in silico 361 ACATCTTCAAGAAGCTCTACAAGGAGACTGC-CTTCGTCC-ACGCGCTCACAGCGGCCGC 420
4698R-2.IR_full 421 CATGACCCACTCGATAGCTAGGGCCTGTGCCGAGGGACGTATGACCAAGTGCAGCTGCGG 480
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
4698R-2.in silico 421 CATGACCCACTCGATAGCCAGGGCCTGTGCCGAGGGACGTATGACCAAGTGCAGCTGCGG 480
4698R-2.IR_full 481 GCCAAAAAAGCACAACAGGGAG 502
||||||||||||||||||||||
4698R-2.in silico 481 GCCAAAAAAGCACAACAGGGAG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057624.2 |
CG4698-RA (Wnt4), mRNA |
| 0 |
0 |
7 |
5 |
9 |
NM_078778.3 |
CG4889-RA, transcript variant A (wg), mRNA |
| 0 |
0 |
7 |
5 |
9 |
NM_164746.1 |
CG4889-RB, transcript variant B (wg), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_080104.2 |
CG4032-RA (Abl), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_136675.1 |
CG1625-RA (CG1625), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_079749.4 |
CG5405-RA, transcript variant A (KrT95D), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_170108.2 |
CG5405-RB, transcript variant B (KrT95D), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_170110.2 |
CG5405-RC, transcript variant C (KrT95D), mRNA |
| 0 |
0 |
3 |
2 |
12 |
NM_205902.1 |
CG3304-RB, transcript variant B (CG3304), mRNA |
| 0 |
0 |
3 |
2 |
12 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
3 |
2 |
12 |
NM_134926.2 |
CG3304-RA, transcript variant A (CG3304), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_169440.1 |
CG6977-RA (Cad87A), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_142947.2 |
CG5933-RA (CG5933), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143536.1 |
CG15537-RA (CG15537), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_132443.1 |
CG16922-RA (CG16922), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_057865.3 |
CG3324-RA (Pkg21D), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_136158.2 |
CG10538-RA (CdGAPr), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_168647.1 |
CG32156-RA, transcript variant A (Mbs), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_168648.1 |
CG32156-RB, transcript variant B (Mbs), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_168646.1 |
CG32156-RC, transcript variant C (Mbs), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_078989.2 |
CG12369-RA, transcript variant A (Lac), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_140280.2 |
CG5688-RA (Grip163), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_138045.2 |
CG4057-RB, transcript variant B (tamo), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166664.1 |
CG4057-RA, transcript variant A (tamo), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_080358.2 |
CG1484-RA (fliI), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_142235.1 |
CG4699-RA, transcript variant A (CG4699), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169672.1 |
CG4699-RB, transcript variant B (CG4699), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169673.1 |
CG4699-RC, transcript variant C (CG4699), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_134997.2 |
CG17840-RA (CG17840), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139788.1 |
CG14911-RA (CG14911), mRNA |
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