NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4696R-1

Symbol Mp20 Full Name Muscle protein 20

CG No CG4696 Old CG No CG4696

Synonyms DMMP20, Dmmp20, CG4696, mp20, Tpn, Mp20

Accession No (Link to NCBI) NM_057295.3

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATGGACAAGGAGGCCCAGGA

Primer Seq. 3'

aatctagaggtaccCTTGCCGAGCAGGATCTTGCG

Predicted Fragment Size

IR fragment full Seq

0001 NATGGACAAG GAGGCCCAGG AGTGGATCGA GGCCATCATT GCCGAGAAGT TCCCCGCCGG 
0061 CCAGTCCTAC GAGGATGTGC TCAAGGACGG TCAGGTGCTG TGCAAACTGA TCAANCGTGC 
0121 TGTCGCCCAA TGCCGTGCCC AAGGTCAACT CCTCGGGCGG CCAGTTCAAG TTCATGGAGA 
0181 ACATCAACAA CTTCCAGAAG GCCCTGAAGG AGTACGGTGT GCCCGACATC GATGTCTTCC 
0241 AGACCGTCGA TCTGTACGAG AAGAAGGATA TTGCCAACGT TACCAACACC ATCTTCGCTT 
0301 TGGGCCGTGC CACCTACAAG CATGCCGACT TCAAGGGTCC CTTCCTGGGC CCCAAGCCCG 
0361 CCGATGAGTG CAAGCGCGAC TTCACCGAAG AGCAGCTGAA GGCTGGCCAG ACCATTGTGG 
0421 GTCTGCAGGC CGGTTCCAAC AAGGGAGCCA CCCAGGCTGG CCAGAACCTC GGCGCTGGCC 
0481 GCAAGATCCT GCTCGGCAAG

in silico PCR Fragment

0001 GATGGACAAG GAGGCCCAGG AGTGGATCGA GGCCATCATT GCCGAGAAGT TCCCCGCCGG 
0061 CCAGTCCTAC GAGGATGTGC TCAAGGACGG TCAGGTGCTG TGCAAACTGA TCAA-CGTGC 
0121 TGTCGCCCAA TGCCGTGCCC AAGGTCAACT CCTCGGGCGG CCAGTTCAAG TTCATGGAGA 
0181 ACATCAACAA CTTCCAGAAG GCCCTGAAGG AGTACGGTGT GCCCGACATC GATGTCTTCC 
0241 AGACCGTCGA TCTGTACGAG AAGAAGGATA TTGCCAACGT TACCAACACC ATCTTCGCTT 
0301 TGGGCCGTGC CACCTACAAG CATGCCGACT TCAAGGGTCC CTTCCTGGGC CCCAAGCCCG 
0361 CCGATGAGTG CAAGCGCGAT TTCACCGAAG AGCAGCTGAA GGCTGGCCAG ACCATTGTGG 
0421 GTCTGCAGGC CGGTTCCAAC AAGGGAGCCA CCCAGGCTGG CCAGAACCTC GGCGCTGGCC 
0481 GCAAGATCCT GCTCGGCAAG

Assemble Data


4696R-1.IR_full        1   ATGGACAAGGAGGCCCAGGAGTGGATCGAGGCCATCATTGCCGAGAAGTTCCCCGCCGG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico      1   ATGGACAAGGAGGCCCAGGAGTGGATCGAGGCCATCATTGCCGAGAAGTTCCCCGCCGG 59


4696R-1.IR_full       61  CCAGTCCTACGAGGATGTGCTCAAGGACGGTCAGGTGCTGTGCAAACTGATCAANCGTGC 119
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
4696R-1.in silico     61  CCAGTCCTACGAGGATGTGCTCAAGGACGGTCAGGTGCTGTGCAAACTGATCAA-CGTGC 119


4696R-1.IR_full       121 TGTCGCCCAATGCCGTGCCCAAGGTCAACTCCTCGGGCGGCCAGTTCAAGTTCATGGAGA 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     121 TGTCGCCCAATGCCGTGCCCAAGGTCAACTCCTCGGGCGGCCAGTTCAAGTTCATGGAGA 179


4696R-1.IR_full       181 ACATCAACAACTTCCAGAAGGCCCTGAAGGAGTACGGTGTGCCCGACATCGATGTCTTCC 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     181 ACATCAACAACTTCCAGAAGGCCCTGAAGGAGTACGGTGTGCCCGACATCGATGTCTTCC 239


4696R-1.IR_full       241 AGACCGTCGATCTGTACGAGAAGAAGGATATTGCCAACGTTACCAACACCATCTTCGCTT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     241 AGACCGTCGATCTGTACGAGAAGAAGGATATTGCCAACGTTACCAACACCATCTTCGCTT 299


4696R-1.IR_full       301 TGGGCCGTGCCACCTACAAGCATGCCGACTTCAAGGGTCCCTTCCTGGGCCCCAAGCCCG 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     301 TGGGCCGTGCCACCTACAAGCATGCCGACTTCAAGGGTCCCTTCCTGGGCCCCAAGCCCG 359


4696R-1.IR_full       361 CCGATGAGTGCAAGCGCGACTTCACCGAAGAGCAGCTGAAGGCTGGCCAGACCATTGTGG 419
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     361 CCGATGAGTGCAAGCGCGATTTCACCGAAGAGCAGCTGAAGGCTGGCCAGACCATTGTGG 419


4696R-1.IR_full       421 GTCTGCAGGCCGGTTCCAACAAGGGAGCCACCCAGGCTGGCCAGAACCTCGGCGCTGGCC 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4696R-1.in silico     421 GTCTGCAGGCCGGTTCCAACAAGGGAGCCACCCAGGCTGGCCAGAACCTCGGCGCTGGCC 479


4696R-1.IR_full       481 GCAAGATCCTGCTCGGCAAG 499
                          ||||||||||||||||||||
4696R-1.in silico     481 GCAAGATCCTGCTCGGCAAG 499

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	481	0	0	0	NM_057295.3	CG4696-RA, transcript variant A (Mp20), mRNA
100	481	0	0	0	NM_001014522.1	CG4696-RB, transcript variant B (Mp20), mRNA
0.2	1	3	4	5	NM_206717.2	CG9220-RC (CG9220), mRNA
0.2	1	2	4	2	NM_165801.1	CG12323-RB, transcript variant B (Prosbeta5), mRNA
0.2	1	2	4	2	NM_143757.3	CG12323-RA, transcript variant A (Prosbeta5), mRNA
0	0	3	10	23	NM_139603.2	CG14996-RB (Chd64), mRNA
0	0	3	2	6	NM_080011.2	CG7035-RB, transcript variant B (Cbp80), mRNA
0	0	3	2	6	NM_167012.1	CG7035-RA, transcript variant A (Cbp80), mRNA
0	0	3	2	4	NM_057357.2	CG1569-RA (rod), mRNA
0	0	2	8	17	NM_078712.2	CG12794-RA (lcs), mRNA
0	0	2	4	25	NM_142610.2	CG5023-RA (CG5023), mRNA
0	0	2	3	3	NM_139844.2	CG8606-RA, transcript variant A (RhoGEF4), mRNA
0	0	2	3	3	NM_168199.1	CG8606-RB, transcript variant B (RhoGEF4), mRNA
0	0	2	3	3	NM_141341.2	CG1208-RA (CG1208), mRNA
0	0	2	3	3	NM_058030.3	CG4581-RA (Thiolase), mRNA
0	0	2	2	2	NM_137628.1	CG13868-RA (CG13868), mRNA
0	0	1	6	3	NM_001043241.1	CG14713-RB (CG14713), mRNA
0	0	1	3	7	NM_139356.1	CG3524-RA (v(2)k05816), mRNA
0	0	1	3	6	NM_169701.2	CG31045-RB, transcript variant B (Mhcl), mRNA
0	0	1	3	6	NM_169700.2	CG31045-RA, transcript variant A (Mhcl), mRNA
0	0	1	3	6	NM_001043250.1	CG31045-RG, transcript variant G (Mhcl), mRNA
0	0	1	3	5	NM_170650.3	CG31045-RC, transcript variant C (Mhcl), mRNA
0	0	1	3	5	NM_001043251.1	CG31045-RF, transcript variant F (Mhcl), mRNA
0	0	1	3	4	NM_136536.2	CG2110-RA (Cyp4ad1), mRNA
0	0	1	3	3	NM_137199.2	CG8182-RA, transcript variant A (GalNAc-T1), mRNA
0	0	1	3	3	NM_166099.1	CG8182-RB, transcript variant B (GalNAc-T1), mRNA
0	0	1	3	3	NM_135559.2	CG5337-RA (CG5337), mRNA
0	0	1	2	5	NM_001043040.1	CG17800-PAJ (Dscam), mRNA
0	0	1	2	4	NM_080107.1	CG15191-RA (e(y)2), mRNA
0	0	1	2	4	NM_001042789.1	CG13366-RB, transcript variant B (CG13366), mRNA

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