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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4694R-4 |
| Symbol |
her |
Full Name |
hermaphrodite |
| CG No |
CG4694 |
Old CG No |
CG4694 |
| Synonyms |
l(2)mat, CG4694, her |
| Accession No (Link to NCBI) |
NM_057224.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal, female abnormal genitalia |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTTAGTGCGGATCGGGATTC |
| Primer Seq. 3' |
aatctagaggtaccGGCGCAGTTCCTCCGCAGTTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCTTAGTGCG GATCGGGATT CGGTGGAGGA AGAATATGGA TCGTGCTCGG CCTGGAAATT
0061 CCGTTGCAGC GTGGACAGGT GCCCCTACAG GACGAACAGA CCCTACAATC TGGCGCGTCA
0121 TGAGGAGTCC CACATTGGTA TTACGCAgTC CAAGCTATAC GGATGCCCCG TTTGCGTTTA
0181 CAACACCGAC AAGGCGTCGA ACCTGAAGCG GCACGTGTCT ATCAAACATC CTGGGTGCaA
0241 ANNGNNNCCT CCGGAAGCGC AGCACAAGGA TCGCAATGCC AAGTTGCAGT GCCTGGTGAT
0301 GGGATGCAGA TACGAGACAA ATCGTCCATA CGACCTCAAG CGCCACTTGA TGGTGCACAA
0361 CAATCCGGAG AAGAGCCACA GGACGTTTAA GTGCTCCCTT TGCACCTACA GCTCCGACCG
0421 AAAGGCCAAT CTCAAGCGGC ACCACGAGCT ACGCGCATTC GGGCATAGAG GAGGCCATTC
0481 AAACTGCGGA GGAACTGCGC C |
| in silico PCR Fragment |
0001 GCTTAGTGCG GATCGGGATT CGGTGGAGGA AGAATATGGA TCGTGCTCGG CCTGGAAATT
0061 CCGTTGCAGC GTGGACAGGT GCCCCTACAG GACGAACAGA CCCTACAATC TGGCGCGTCA
0121 TGAGGAGTCC CACATTGGTA TTACGCAGTC CAAGCTATAC GGATGCCCCG TTTGCGTTTA
0181 CAACACCGAC AAGGCGTCGA ACCTGAAGCG GCACGTGTCT ATCAAACATC CTGGGTGCAA
0241 AAAGCGCCCT CCGGAAGCGC AGCACAAGGA TCGCAATGCC AAGTTGCAGT GCCTGGTGAT
0301 GGGATGCAGA TACGAGACAA ATCGTCCATA CGACCTCAAG CGCCACTTGA TGGTGCACAA
0361 CAATCCGGAG AAGAGCCACA GGACGTTTAA GTGCTCCCTT TGCACCTACA GCTCCGACCG
0421 AAAGGCCAAT CTCAAGCGGC ACCACGAGCT ACGC-CATTC GGGCATAGAG GAGGCCATTC
0481 AAACTGCGGA GGAACTGCGC C |
| Assemble Data |
4694R-4.IR_full 1 GCTTAGTGCGGATCGGGATTCGGTGGAGGAAGAATATGGATCGTGCTCGGCCTGGAAATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 1 GCTTAGTGCGGATCGGGATTCGGTGGAGGAAGAATATGGATCGTGCTCGGCCTGGAAATT 60
4694R-4.IR_full 61 CCGTTGCAGCGTGGACAGGTGCCCCTACAGGACGAACAGACCCTACAATCTGGCGCGTCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 61 CCGTTGCAGCGTGGACAGGTGCCCCTACAGGACGAACAGACCCTACAATCTGGCGCGTCA 120
4694R-4.IR_full 121 TGAGGAGTCCCACATTGGTATTACGCAGTCCAAGCTATACGGATGCCCCGTTTGCGTTTA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 121 TGAGGAGTCCCACATTGGTATTACGCAGTCCAAGCTATACGGATGCCCCGTTTGCGTTTA 180
4694R-4.IR_full 181 CAACACCGACAAGGCGTCGAACCTGAAGCGGCACGTGTCTATCAAACATCCTGGGTGCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 181 CAACACCGACAAGGCGTCGAACCTGAAGCGGCACGTGTCTATCAAACATCCTGGGTGCAA 240
4694R-4.IR_full 241 ANNGNNNCCTCCGGAAGCGCAGCACAAGGATCGCAATGCCAAGTTGCAGTGCCTGGTGAT 300
| | |||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 241 AAAGCGCCCTCCGGAAGCGCAGCACAAGGATCGCAATGCCAAGTTGCAGTGCCTGGTGAT 300
4694R-4.IR_full 301 GGGATGCAGATACGAGACAAATCGTCCATACGACCTCAAGCGCCACTTGATGGTGCACAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 301 GGGATGCAGATACGAGACAAATCGTCCATACGACCTCAAGCGCCACTTGATGGTGCACAA 360
4694R-4.IR_full 361 CAATCCGGAGAAGAGCCACAGGACGTTTAAGTGCTCCCTTTGCACCTACAGCTCCGACCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4694R-4.in silico 361 CAATCCGGAGAAGAGCCACAGGACGTTTAAGTGCTCCCTTTGCACCTACAGCTCCGACCG 420
4694R-4.IR_full 421 AAAGGCCAATCTCAAGCGGCACCACGAGCTACGCGCATTCGGGCATAGAGGAGGCCATTC 480
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
4694R-4.in silico 421 AAAGGCCAATCTCAAGCGGCACCACGAGCTACGC-CATTCGGGCATAGAGGAGGCCATTC 480
4694R-4.IR_full 481 AAACTGCGGAGGAACTGCGCC 501
|||||||||||||||||||||
4694R-4.in silico 481 AAACTGCGGAGGAACTGCGCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057224.3 |
CG4694-RA (her), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_165215.1 |
CG31803-RA (CG31803), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_136044.2 |
CG10333-RA (CG10333), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_166790.2 |
CG32019-RA, transcript variant A (bt), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_205877.1 |
CG32019-RD, transcript variant D (bt), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_205876.1 |
CG32019-RC, transcript variant C (bt), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_205875.1 |
CG32019-RE, transcript variant E (bt), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139790.1 |
CG8270-RA (CG8270), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_164454.1 |
CG31671-RA (tho2), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_135011.2 |
CG15631-RA (CG15631), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079589.2 |
CG6649-RA (Ugt35b), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132777.1 |
CG9091-RA (RpL37a), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_057406.3 |
CG6222-RA (su(s)), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132567.1 |
CG15732-RA (CG15732), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137047.2 |
CG6197-RA (CG6197), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165468.1 |
CG8276-RA, transcript variant A (bin3), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_078902.2 |
CG8276-RB, transcript variant B (bin3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137497.2 |
CG10924-RA (CG10924), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132503.2 |
CG11696-RA (CG11696), mRNA |
| 0 |
0 |
0 |
6 |
15 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_134987.1 |
CG15431-RA (CG15431), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001015171.1 |
CG41066-PA.3 (CG41066), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_137116.2 |
CG8422-RA (CG8422), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_170247.1 |
CG17367-RC, transcript variant C (Lnk), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_143181.2 |
CG17367-RB, transcript variant B (Lnk), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_170246.1 |
CG17367-RA, transcript variant A (Lnk), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_058035.2 |
CG8717-RA (slv), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_169665.1 |
CG18519-RB, transcript variant B (CG18519), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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| Dcompartment |
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Penetrance |
| + |
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