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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4686R-1 |
| Symbol |
CG4686 |
Full Name |
CG4686 |
| CG No |
CG4686 |
Old CG No |
CG4686 |
| Synonyms |
BcDNA:AT09463, CG4686 |
| Accession No (Link to NCBI) |
NM_142580.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGAATACGTGACCCTGGGCA |
| Primer Seq. 3' |
aatctagaggtaccCATCAGGCAGAATCCTCCGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGAATACGT GACCCTGGGC AATCCTGTCA GCAAGATGGT AGCCTCGTCC GCATNCCGCN
0061 CCCTGCTCCG CACGCTTGGT CTGCGTCCCA AGAAGGTGCC GGTGCAGGAG ACGAGTATGG
0121 CGGTGCTCCC TGCCGCCCAC AGCTACGCGC ATTCACACGG ATCACTCTAT CGACTGGCCG
0181 GCTGCCATTA CCACTTCATT CGGCTGGCCG GGATCGTCGG CGCGTCGGCC ATCTTTATGG
0241 GCGCCTACTG CAAGTACGTC CTGAAGGACG TCAGCGATCC CAAGGAGCAG GTGGACTCGC
0301 AGGCCTTTGC TGATGTGGCC AATCGCATCC ACTTTCTGCA CTCCTTTGCC ATGATGGCCN
0361 ATGCCTCTGG CCCACTATCC CGTATTCACT GGCACTTTGA TGATTACGGG CATGATGCTT
0421 TTCAGCGGCT GCATGTACTA CCGCGCTTTG ACTGGCGAGA AGCGTCTGCA ACCGTACGCG
0481 ACCGTCGGAG GATTCTGCCT GATG |
| in silico PCR Fragment |
0001 TTGAATACGT GACCCTGGGC AATCCTGTCA GCAAGATGGT AGCCTCGTCC GCAT-CCG--
0061 CCCTGCTCCG CACGCTTGGT CTGCGTCCCA AGAAGGTGCC GGTGCAGGAG ACGAGTATGG
0121 CGGTGCTCCC TGCCGCCCAC AGCTACGCGC ATTCACACGG ATCACTCTAT CGACTGGCCG
0181 GCTGCCATTA CCACTTCATT CGGCTGGCCG GGATCGTCGG CGCGTCGGCC ATCTTTATGG
0241 GCGCCTACTG CAAGTACGTC CTGAAGGACG TCAGCGATCC CAAGGAGCAG GTGGACTCGC
0301 AGGCCTTTGC TGATGTGGCC AATCGCATCC ACTTTCTGCA CTCCTTTGCC ATGATGGCC-
0361 ATGCCTCTGG CCCACTATCC CGTATTCACT GGCACTTTGA TGATTACGGG CATGATGCTT
0421 TTCAGCGGCT GCATGTACTA CCGCGCTTTG ACTGGCGAGA AGCGTCTGCA ACCGTACGCG
0481 ACCGTCGGAG GATTCTGCCT GATG |
| Assemble Data |
4686R-1.IR_full 1 TTGAATACGTGACCCTGGGCAATCCTGTCAGCAAGATGGTAGCCTCGTCCGCATNCCGCN 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
4686R-1.in silico 1 TTGAATACGTGACCCTGGGCAATCCTGTCAGCAAGATGGTAGCCTCGTCCGCAT-CCG-- 60
4686R-1.IR_full 61 CCCTGCTCCGCACGCTTGGTCTGCGTCCCAAGAAGGTGCCGGTGCAGGAGACGAGTATGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 61 CCCTGCTCCGCACGCTTGGTCTGCGTCCCAAGAAGGTGCCGGTGCAGGAGACGAGTATGG 120
4686R-1.IR_full 121 CGGTGCTCCCTGCCGCCCACAGCTACGCGCATTCACACGGATCACTCTATCGACTGGCCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 121 CGGTGCTCCCTGCCGCCCACAGCTACGCGCATTCACACGGATCACTCTATCGACTGGCCG 180
4686R-1.IR_full 181 GCTGCCATTACCACTTCATTCGGCTGGCCGGGATCGTCGGCGCGTCGGCCATCTTTATGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 181 GCTGCCATTACCACTTCATTCGGCTGGCCGGGATCGTCGGCGCGTCGGCCATCTTTATGG 240
4686R-1.IR_full 241 GCGCCTACTGCAAGTACGTCCTGAAGGACGTCAGCGATCCCAAGGAGCAGGTGGACTCGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 241 GCGCCTACTGCAAGTACGTCCTGAAGGACGTCAGCGATCCCAAGGAGCAGGTGGACTCGC 300
4686R-1.IR_full 301 AGGCCTTTGCTGATGTGGCCAATCGCATCCACTTTCTGCACTCCTTTGCCATGATGGCCN 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 301 AGGCCTTTGCTGATGTGGCCAATCGCATCCACTTTCTGCACTCCTTTGCCATGATGGCC- 360
4686R-1.IR_full 361 ATGCCTCTGGCCCACTATCCCGTATTCACTGGCACTTTGATGATTACGGGCATGATGCTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 361 ATGCCTCTGGCCCACTATCCCGTATTCACTGGCACTTTGATGATTACGGGCATGATGCTT 420
4686R-1.IR_full 421 TTCAGCGGCTGCATGTACTACCGCGCTTTGACTGGCGAGAAGCGTCTGCAACCGTACGCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4686R-1.in silico 421 TTCAGCGGCTGCATGTACTACCGCGCTTTGACTGGCGAGAAGCGTCTGCAACCGTACGCG 480
4686R-1.IR_full 481 ACCGTCGGAGGATTCTGCCTGATG 504
||||||||||||||||||||||||
4686R-1.in silico 481 ACCGTCGGAGGATTCTGCCTGATG 504
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_142580.1 |
CG4686-RA (CG4686), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_130531.2 |
CG11384-RA (CG11384), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_080250.2 |
CG6224-RA (dbo), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_138188.1 |
CG1231-RA (CG1231), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165712.1 |
CG1516-RL, transcript variant L (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165708.1 |
CG1516-RG, transcript variant G (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165711.1 |
CG1516-RK, transcript variant K (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165706.1 |
CG1516-RB, transcript variant B (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165707.1 |
CG1516-RD, transcript variant D (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165710.1 |
CG1516-RJ, transcript variant J (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165705.1 |
CG1516-RA, transcript variant A (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165709.1 |
CG1516-RI, transcript variant I (CG1516), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136683.2 |
CG1516-RE, transcript variant E (CG1516), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_079818.2 |
CG10002-RA (fkh), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_057324.3 |
CG5109-RA (Pcl), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_169826.1 |
CG31224-RA (CG31224), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_167858.1 |
CG32335-RA (CG32335), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078992.2 |
CG8581-RA, transcript variant A (fra), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165913.1 |
CG8581-RB, transcript variant B (fra), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_206420.1 |
CG33291-RA (CG33291), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_143093.2 |
CG13654-RA (CG13654), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142669.2 |
CG10830-RA (CG10830), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057819.3 |
CG9258-RA (nrv1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057950.2 |
CG2054-RA (Cht2), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_142179.1 |
CG6499-RA (CG6499), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_139786.1 |
CG13300-RA (CG13300), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_135371.3 |
CG7627-RA (CG7627), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167735.1 |
CG32521-RB, transcript variant B (CG32521), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167734.1 |
CG32521-RA, transcript variant A (CG32521), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167736.1 |
CG32521-RC, transcript variant C (CG32521), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
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