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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4646R-1 
 Symbol CG4646  Full Name CG4646 
 CG No CG4646  Old CG No CG4646 
 Synonyms BcDNA:SD14289, CG4646 
 Accession No (Link to NCBI) NM_137004.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTCGTGTGGGCCTTCAGATTT 
 Primer Seq. 3' 
aatctagaggtaccAGCTTAATGAACTTCGAGGCG 
 Predicted Fragment Size 
478 
 IR fragment full Seq 
0001 TTCGTGTGGG CCTTCAGATT TCCGCCACGC TGGAGAACGT GGACAAGCTG GAGACGTCTC 
0061 ATCCGGACTA CTCCTTCTTC TTGAAGCTGA AGTGCAGCAA CTGCGGCGAG CAGTCGGACA 
0121 AGTGGCACGA TATCACGGAA TCCGAACGTG TGCAACAGGA TTCGCGCAAT GCGGCCGGCT 
0181 TTAACTTCTT CATGAAGTGC AAGATGTGCA GCCGGGAGAA CAGCATCGAC ATCGTCGACA 
0241 AGTCGAATGC TCCCTACACG GCGGACGATT CTGGCGCATT TAAGACCATC GTGGTCTTCG 
0301 AGTGCCGCGG CGCAGAGTCC CGTCGAATTC CNTCGCCGCG CGTCGGTTGG CGCGTCTCAT 
0361 CCGCTGAAAA TGGCCAGCAG TTCGAGGAGG TGGACCTGTC CGAGGACGAC TGGGTGGAGT 
0421 ACGACCAGAA GAACAACAAC TCTGTGGGCA TTTATGAATT CGCCTCGAAG TTCATTAAGC 
0481 T 
 in silico PCR Fragment 
0001 TTCGTGTGGG CCTTCAGATT TCCGCCACGC TGGAGAACGT GGACAAGCTG GAGACGTCTC 
0061 ATCCGGACTA CTCCTTCTTC TTGAAGCTGA AGTGCAGCAA CTGCGGCGAG CAGTCGGACA 
0121 AGTGGCACGA TATCACGGAA TCCGAACGTG TGCAACAGGA TTCGCGCAAT GCGGCCGGCT 
0181 TTAACTTCTT CATGAAGTGC AAGATGTGCA GCCGGGAGAA CAGCATCGAC ATCGTCGACA 
0241 AGTCGAATGC TCCCTACACG GCGGACGATT CTGGCGCATT TAAGACCATC GTGGTCTTCG 
0301 AGTGCCGCGG CGCAGAG-CC CGTCGAATTC --TCGCCGCG CGTCGGTTGG CGCGTCTCAT 
0361 CCGCTGAAAA TGGCCAGCAG TTCGAGGAGG TGGACCTGTC CGAGGACGAC TGGGTGGAGT 
0421 ACGACCAGAA GAACAACAAC TCTGTGGGCA TTTATGAATT CGCCTCGAAG TTCATTAAGC 
0481 T 
 Assemble Data 

4646R-1.IR_full       1   TTCGTGTGGGCCTTCAGATTTCCGCCACGCTGGAGAACGTGGACAAGCTGGAGACGTCTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     1   TTCGTGTGGGCCTTCAGATTTCCGCCACGCTGGAGAACGTGGACAAGCTGGAGACGTCTC 60


4646R-1.IR_full       61  ATCCGGACTACTCCTTCTTCTTGAAGCTGAAGTGCAGCAACTGCGGCGAGCAGTCGGACA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     61  ATCCGGACTACTCCTTCTTCTTGAAGCTGAAGTGCAGCAACTGCGGCGAGCAGTCGGACA 120


4646R-1.IR_full       121 AGTGGCACGATATCACGGAATCCGAACGTGTGCAACAGGATTCGCGCAATGCGGCCGGCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     121 AGTGGCACGATATCACGGAATCCGAACGTGTGCAACAGGATTCGCGCAATGCGGCCGGCT 180


4646R-1.IR_full       181 TTAACTTCTTCATGAAGTGCAAGATGTGCAGCCGGGAGAACAGCATCGACATCGTCGACA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     181 TTAACTTCTTCATGAAGTGCAAGATGTGCAGCCGGGAGAACAGCATCGACATCGTCGACA 240


4646R-1.IR_full       241 AGTCGAATGCTCCCTACACGGCGGACGATTCTGGCGCATTTAAGACCATCGTGGTCTTCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     241 AGTCGAATGCTCCCTACACGGCGGACGATTCTGGCGCATTTAAGACCATCGTGGTCTTCG 300


4646R-1.IR_full       301 AGTGCCGCGGCGCAGAGTCCCGTCGAATTCCNTCGCCGCGCGTCGGTTGGCGCGTCTCAT 360
                          ||||||||||||||||| ||||||||||||  ||||||||||||||||||||||||||||
4646R-1.in silico     301 AGTGCCGCGGCGCAGAG-CCCGTCGAATTC--TCGCCGCGCGTCGGTTGGCGCGTCTCAT 360


4646R-1.IR_full       361 CCGCTGAAAATGGCCAGCAGTTCGAGGAGGTGGACCTGTCCGAGGACGACTGGGTGGAGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     361 CCGCTGAAAATGGCCAGCAGTTCGAGGAGGTGGACCTGTCCGAGGACGACTGGGTGGAGT 420


4646R-1.IR_full       421 ACGACCAGAAGAACAACAACTCTGTGGGCATTTATGAATTCGCCTCGAAGTTCATTAAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4646R-1.in silico     421 ACGACCAGAAGAACAACAACTCTGTGGGCATTTATGAATTCGCCTCGAAGTTCATTAAGC 480


4646R-1.IR_full       481 T 481
                          |
4646R-1.in silico     481 T 481



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   460  0  0  0  NM_137004.2  CG4646-RA (CG4646), mRNA 
0   0  7  2  6  NM_080152.2  CG5092-RA (Tor), mRNA 
0   0  6  3  2  NM_137414.2  CG4996-RA (CG4996), mRNA 
0   0  3  4  3  NM_134700.2  CG3561-RA (CG3561), mRNA 
0   0  2  7  5  NM_001038734.1  CG16902-RC (Hr4), mRNA 
0   0  2  4  4  NM_079096.2  CG9889-RA (yellow-d), mRNA 
0   0  2  4  2  NM_143454.1  CG7584-RA (Obp99c), mRNA 
0   0  2  3  3  NM_080110.2  CG32356-RA, transcript variant A (ImpE1), mRNA 
0   0  2  3  2  NM_164601.1  CG2837-RA, transcript variant A (CG2837), mRNA 
0   0  2  3  2  NM_164602.1  CG2837-RC, transcript variant C (CG2837), mRNA 
0   0  2  3  2  NM_135030.2  CG2837-RB, transcript variant B (CG2837), mRNA 
0   0  2  2  6  NM_078664.2  CG5870-RA (beta-Spec), mRNA 
0   0  2  2  5  NM_133114.2  CG32541-RA (CG32541), mRNA 
0   0  1  3  9  NM_143511.2  CG7946-RA (CG7946), mRNA 
0   0  1  3  8  NM_132941.2  CG4805-RA, transcript variant A (ppk28), mRNA 
0   0  1  3  8  NM_001014748.1  CG4805-RB, transcript variant B (ppk28), mRNA 
0   0  1  3  4  NM_001014461.1  CG9967-RB, transcript variant B (CG9967), mRNA 
0   0  1  3  4  NM_078583.2  CG2559-RA (Lsp1alpha), mRNA 
0   0  1  3  4  NM_057276.3  CG4178-RA (Lsp1beta), mRNA 
0   0  1  2  3  NM_135084.1  CG14020-RA (CG14020), mRNA 
0   0  1  2  2  NM_176737.1  CG9213-RA (CG9213), mRNA 
0   0  1  2  1  NM_143322.1  CG12883-RA (CG12883), mRNA 
0   0  0  8  6  NM_166647.2  CG3907-RA, transcript variant A (CG3907), mRNA 
0   0  0  8  6  NM_138026.3  CG3907-RB, transcript variant B (CG3907), mRNA 
0   0  0  7  7  NM_057269.2  CG10236-RA (LanA), mRNA 
0   0  0  6  7  NM_079502.2  CG1059-RA (Karybeta3), mRNA 
0   0  0  6  6  NM_141398.1  CG10272-RA, transcript variant A (gpp), mRNA 
0   0  0  6  6  NM_169144.1  CG10272-RC, transcript variant C (gpp), mRNA 
0   0  0  6  6  NM_169143.1  CG10272-RD, transcript variant D (gpp), mRNA 
0   0  0  6  6  NM_169142.1  CG10272-RB, transcript variant B (gpp), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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