|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4591R-1 |
| Symbol |
Tsp86D |
Full Name |
Tetraspanin 86D |
| CG No |
CG4591 |
Old CG No |
CG4591 |
| Synonyms |
CG4591, Dm.Tsp86D, Tsp86D |
| Accession No (Link to NCBI) |
NM_079585.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Jeibmann A, Halama K, Witte HT, Kim SN, Eikmeier K, Koos B, Klämbt C, Paulus W.
Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
J Neurooncol (2015) 124(3) 373-83
[
PubMed ID = 26224160
]
[
RRC reference
]
Dornier E, Coumailleau F, Ottavi JF, Moretti J, Boucheix C, Mauduit P, Schweisguth F, Rubinstein E.
TspanC8 tetraspanins regulate ADAM10/Kuzbanian trafficking and promote Notch activation in flies and mammals.
J Cell Biol (2012) 199(3) 481-96
[
PubMed ID = 23091066
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAATCACAGATACCATCAGGG |
| Primer Seq. 3' |
aatctagaggtaccGCTGGGCAAAGTCGATGAAGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAATCACAGA TACCATCAGG GGGGCAACTA CATGCACCCG CGGATCTCCa NCTATCCGCA
0061 CCACTTCAGC TACGTTTCCT CTTGCGTCAA GTACATGATC TTCCTGCTAA ACTTCCTCTT
0121 CTGGCTCTTC GGCGGCCTGC TCCTGGCCAT CGGCGTGNTA CGCCTTCATG GACAAACTGA
0181 TGGACGGCAA TGGCTGGCTG CGCTTGGACA CCATCTACGA TGTGATCTTC AACATCTCCC
0241 TGGTGATGAT CATCGCCGGA GTCATCGTCT TCACGGTCAg cTTCGCCGGC TGTTTGGgtN
0301 NGCTNCGCGA GAACACCTGG CTGCTAAAGC TGTACTCCAT GTGCCTGCTT CTCTTCTTCA
0361 TACTGGAGAT GTCGCTGGCC ATCATCTGCT TCGTTTTCCC GCAGTACATG AACTCGTTCC
0421 TCGAGTACCA GTTCACGGAC AAAATCATCC ACTCGTACCG TGACGACTCC GACTTGCAAA
0481 ACTTCATCGA CTTTGCCCAG C |
| in silico PCR Fragment |
0001 CAATCACAGA TACCATCAGG GGGGCAACTA CATGCACCCG CGGATCTCCA CCTATCCGCA
0061 CCACTTCAGC TACGTTTCCT CTTGCGTCAA GTACATGATC TTCCTGCTAA ACTTCCTCTT
0121 CTGGCTCTTC GGCGGCCTGC TCCTGGCCAT CGGCGTG-TA CGCCTTCATG GACAAACTGA
0181 TGGACGGCAA TGGCTGGCTG CGCTTGGACA CCATCTACGA TGTGATCTTC AACATCTCCC
0241 TGGTGATGAT CATCGCCGGA GTCATCGTCT TCACGGTCAG CTTCGCCGGC TGTTTGGGTG
0301 CGCTGCGCGA GAACACCTGG CTGCTAAAGC TGTACTCCAT GTGCCTGCTT CTCTTCTTCA
0361 TACTGGAGAT GTCGCTGGCC ATCATCTGCT TCGTTTTCCC GCAGTACATG AACTCGTTCC
0421 TCGAGTACCA GTTCACGGAC AAAATCATCC ACTCGTACCG TGACGACTCC GACTTGCAAA
0481 ACTTCATCGA CTTTGCCCAG C |
| Assemble Data |
4591R-1.IR_full 1 CAATCACAGATACCATCAGGGGGGCAACTACATGCACCCGCGGATCTCCANCTATCCGCA 60
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
4591R-1.in silico 1 CAATCACAGATACCATCAGGGGGGCAACTACATGCACCCGCGGATCTCCACCTATCCGCA 60
4591R-1.IR_full 61 CCACTTCAGCTACGTTTCCTCTTGCGTCAAGTACATGATCTTCCTGCTAAACTTCCTCTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 61 CCACTTCAGCTACGTTTCCTCTTGCGTCAAGTACATGATCTTCCTGCTAAACTTCCTCTT 120
4591R-1.IR_full 121 CTGGCTCTTCGGCGGCCTGCTCCTGGCCATCGGCGTGNTACGCCTTCATGGACAAACTGA 180
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
4591R-1.in silico 121 CTGGCTCTTCGGCGGCCTGCTCCTGGCCATCGGCGTG-TACGCCTTCATGGACAAACTGA 180
4591R-1.IR_full 181 TGGACGGCAATGGCTGGCTGCGCTTGGACACCATCTACGATGTGATCTTCAACATCTCCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 181 TGGACGGCAATGGCTGGCTGCGCTTGGACACCATCTACGATGTGATCTTCAACATCTCCC 240
4591R-1.IR_full 241 TGGTGATGATCATCGCCGGAGTCATCGTCTTCACGGTCAGCTTCGCCGGCTGTTTGGGTN 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 241 TGGTGATGATCATCGCCGGAGTCATCGTCTTCACGGTCAGCTTCGCCGGCTGTTTGGGTG 300
4591R-1.IR_full 301 GCTNCGCGAGAACACCTGGCTGCTAAAGCTGTACTCCATGTGCCTGCTTCTCTTCTTCA 359
||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 301 GCTGCGCGAGAACACCTGGCTGCTAAAGCTGTACTCCATGTGCCTGCTTCTCTTCTTCA 359
4591R-1.IR_full 361 TACTGGAGATGTCGCTGGCCATCATCTGCTTCGTTTTCCCGCAGTACATGAACTCGTTCC 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 361 TACTGGAGATGTCGCTGGCCATCATCTGCTTCGTTTTCCCGCAGTACATGAACTCGTTCC 419
4591R-1.IR_full 421 TCGAGTACCAGTTCACGGACAAAATCATCCACTCGTACCGTGACGACTCCGACTTGCAAA 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4591R-1.in silico 421 TCGAGTACCAGTTCACGGACAAAATCATCCACTCGTACCGTGACGACTCCGACTTGCAAA 479
4591R-1.IR_full 481 ACTTCATCGACTTTGCCCAGC 500
|||||||||||||||||||||
4591R-1.in silico 481 ACTTCATCGACTTTGCCCAGC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_079585.2 |
CG4591-RA (Tsp86D), mRNA |
| 1.65 |
8 |
36 |
83 |
76 |
NM_080315.2 |
CG10742-RA (Tsp3A), mRNA |
| 0 |
0 |
3 |
61 |
176 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_132859.2 |
CG9066-RA (CG9066), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_142275.1 |
CG17929-RA (CG17929), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_057507.3 |
CG8442-RA (Glu-RI), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_143054.2 |
CG11168-RA (CG11168), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142481.1 |
CG7698-RA (CG7698), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132000.2 |
CG12730-RA (CG12730), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079067.2 |
CG11949-RA, transcript variant A (cora), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_078763.1 |
CG9093-RA (Tsp26A), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_206455.1 |
CG33323-RA (Fer1), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_176161.1 |
CG33007-RA (CG33007), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_132104.2 |
CG3226-RA (CG3226), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_144350.1 |
CG7487-RA (RecQ4), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168041.1 |
CG32263-RA (CG32263), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167950.1 |
CG1146-RB, transcript variant B (CG1146), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167951.1 |
CG1146-RC, transcript variant C (CG1146), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139468.1 |
CG1146-RA, transcript variant A (CG1146), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176195.1 |
CG33017-RB (CG33017), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079278.2 |
CG3922-RB (RpS17), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136857.2 |
CG12443-RA (ths), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164933.1 |
CG7438-RB, transcript variant B (Myo31DF), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_078814.2 |
CG7438-RA, transcript variant A (Myo31DF), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143510.1 |
CG15528-RA (CG15528), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_166140.1 |
CG8421-RA, transcript variant A (Asph), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_166141.2 |
CG8421-RD, transcript variant D (Asph), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079033.2 |
CG8421-RB, transcript variant B (Asph), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165747.2 |
CG4001-RC, transcript variant C (Pfk), mRNA |
|
|
|
