NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4586R-3

Symbol CG4586 Full Name CG4586

CG No CG4586 Old CG No CG4586

Synonyms CG4586

Accession No (Link to NCBI) NM_132143.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGGCGTTTCTATGAGGATCCC

Primer Seq. 3'

aatctagaggtaccATGGCCACATTGGCGGTATTA

Predicted Fragment Size

IR fragment full Seq

0001 AGGCGTTTCT ATGAGGATCC CGACTTCGCG NAgcNAGATG CATCCGAGCT GTCTGTCATA 
0061 CAAGGAGCGC TACGAACAGA CCGTGGCCCG ATCCACACGC TTCCTGGCCA AACTGCGCCA 
0121 ATGGCAGCGG GAGAAGCAGC CACAACTTCA GCTTAGTGTG AATATGCTGA GGGACTTTCG 
0181 ACTGCTACTC TCCGGATCCC TGGGCACGGG CCTCTTCCAG CAGAGCTTCC CACTTCGGGT 
0241 GCATTTCTCC ATGTTCATGA CCACCTTGCT GAGCCAGGGA ACCGAGGAGC AGCAGGCCCA 
0301 GTGGCTCAAT CGATCCTGGC ACATGGACGG CGTCCTGGGC ACCTATGCGC AAACGGAGCT 
0361 GGGTCACGGC ACCTTTGTCC GGGGCCTGGA AACCCGAGCC GACTACGATC CCATCACGCA 
0421 GGAATTCATC CTGAACACCC CAACGCAAAG TTCATACAAG TGGTGGCCCG GCGGACTTGG 
0481 TAATACCGCC AATGTGGCCA T

in silico PCR Fragment

0001 AGGCGTTTCT ATGAGGATCC CGACTTCGCG GAGCCAG-TG CATCCGAGCT GTCTGTCATA 
0061 CAAGGAGCGC TACGAACAGA CCGTGGCCCG ATCCACACGC TTCCTGGCCA AACTGCGCCA 
0121 ATGGCAGCGG GAGAAGCAGC CACAACTTCA GCTTAGTGTG AATATGCTGA GGGACTTTCG 
0181 GCTGCTACTC TCCGGATCCC TGGGCACGGG CCTCTTCCAG CAGAGCTTCC CCCTTCGGGT 
0241 GCATTTCTCC ATGTTCATGA CCACCTTGCT GAGCCAGGGA ACCGAGGAGC AGCAGGCCCA 
0301 GTGGCTCAAT CGATCCTGGC ACATGGACGG CGTCCTGGGC ACCTATGCGC AAACGGAGCT 
0361 GGGTCACGGC ACCTTTGTCC GGGGCCTGGA AACCCGAGCC GACTACGATC CCATCACGCA 
0421 GGAATTCATC CTGAACACCC CAACGCAAAG TTCATACAAG TGGTGGCCCG GCGGACTTGG 
0481 TAATACCGCC AATGTGGCCA T

Assemble Data


4586R-3.IR_full       1   AGGCGTTTCTATGAGGATCCCGACTTCGCGNAGCNAGATGCATCCGAGCTGTCTGTCATA 60
                          |||||||||||||||||||||||||||||| ||| || ||||||||||||||||||||||
4586R-3.in silico     1   AGGCGTTTCTATGAGGATCCCGACTTCGCGGAGCCAG-TGCATCCGAGCTGTCTGTCATA 60


4586R-3.IR_full       61  CAAGGAGCGCTACGAACAGACCGTGGCCCGATCCACACGCTTCCTGGCCAAACTGCGCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     61  CAAGGAGCGCTACGAACAGACCGTGGCCCGATCCACACGCTTCCTGGCCAAACTGCGCCA 120


4586R-3.IR_full       121 ATGGCAGCGGGAGAAGCAGCCACAACTTCAGCTTAGTGTGAATATGCTGAGGGACTTTCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     121 ATGGCAGCGGGAGAAGCAGCCACAACTTCAGCTTAGTGTGAATATGCTGAGGGACTTTCG 180


4586R-3.IR_full       181 ACTGCTACTCTCCGGATCCCTGGGCACGGGCCTCTTCCAGCAGAGCTTCCCACTTCGGGT 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
4586R-3.in silico     181 GCTGCTACTCTCCGGATCCCTGGGCACGGGCCTCTTCCAGCAGAGCTTCCCCCTTCGGGT 240


4586R-3.IR_full       241 GCATTTCTCCATGTTCATGACCACCTTGCTGAGCCAGGGAACCGAGGAGCAGCAGGCCCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     241 GCATTTCTCCATGTTCATGACCACCTTGCTGAGCCAGGGAACCGAGGAGCAGCAGGCCCA 300


4586R-3.IR_full       301 GTGGCTCAATCGATCCTGGCACATGGACGGCGTCCTGGGCACCTATGCGCAAACGGAGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     301 GTGGCTCAATCGATCCTGGCACATGGACGGCGTCCTGGGCACCTATGCGCAAACGGAGCT 360


4586R-3.IR_full       361 GGGTCACGGCACCTTTGTCCGGGGCCTGGAAACCCGAGCCGACTACGATCCCATCACGCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     361 GGGTCACGGCACCTTTGTCCGGGGCCTGGAAACCCGAGCCGACTACGATCCCATCACGCA 420


4586R-3.IR_full       421 GGAATTCATCCTGAACACCCCAACGCAAAGTTCATACAAGTGGTGGCCCGGCGGACTTGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4586R-3.in silico     421 GGAATTCATCCTGAACACCCCAACGCAAAGTTCATACAAGTGGTGGCCCGGCGGACTTGG 480


4586R-3.IR_full       481 TAATACCGCCAATGTGGCCAT 501
                          |||||||||||||||||||||
4586R-3.in silico     481 TAATACCGCCAATGTGGCCAT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	1	2	NM_132143.1	CG4586-RA (CG4586), mRNA
0.2	1	10	14	33	NM_137420.2	CG5009-RA (CG5009), mRNA
0	0	6	15	10	NM_079078.2	CG9707-RA (Acox57D-p), mRNA
0	0	5	3	2	NM_141496.1	CG3250-RA (Os-C), mRNA
0	0	3	3	4	NM_134671.1	CG11592-RA (CG11592), mRNA
0	0	2	10	16	NM_079079.2	CG9709-RA (Acox57D-d), mRNA
0	0	2	4	5	NM_164553.1	CG2818-RB, transcript variant B (CG2818), mRNA
0	0	2	4	5	NM_134960.2	CG2818-RA, transcript variant A (CG2818), mRNA
0	0	2	3	5	NM_176181.1	CG8291-RC, transcript variant C (CG8291), mRNA
0	0	2	3	5	NM_137220.2	CG8291-RA, transcript variant A (CG8291), mRNA
0	0	2	3	5	NM_166121.2	CG8291-RB, transcript variant B (CG8291), mRNA
0	0	2	3	2	NM_142963.2	CG6173-RA (kal-1), mRNA
0	0	2	2	2	NM_205956.1	CG33302-RA (CG33302), mRNA
0	0	1	4	7	NM_132116.1	CG14441-RA (CG14441), mRNA
0	0	1	4	5	NM_078698.2	CG1676-RA (cactin), mRNA
0	0	1	4	5	NM_001014585.1	CG6169-RD, transcript variant D (CG6169), mRNA
0	0	1	4	5	NM_206378.1	CG6169-RC, transcript variant C (CG6169), mRNA
0	0	1	4	5	NM_168634.3	CG6169-RB, transcript variant B (CG6169), mRNA
0	0	1	4	5	NM_140548.3	CG6169-RA, transcript variant A (CG6169), mRNA
0	0	1	3	11	NM_057685.3	CG11527-RA (Tig), mRNA
0	0	1	3	8	NM_130555.1	CG14772-RA (CG14772), mRNA
0	0	1	3	4	NM_001042820.1	CG34145-RA (CG34145), mRNA
0	0	1	3	4	NM_134912.1	CG15404-RA (CG15404), mRNA
0	0	1	3	3	NM_141641.2	CG8149-RA (CG8149), mRNA
0	0	1	3	2	NM_136652.2	CG1809-RA (CG1809), mRNA
0	0	1	3	2	NM_142139.1	CG3610-RA (CG3610), mRNA
0	0	1	3	2	NM_141820.1	CG17230-RA, transcript variant A (CG17230), mRNA
0	0	1	3	2	NM_169394.1	CG17230-RB, transcript variant B (CG17230), mRNA
0	0	1	3	2	NM_078584.2	CG1848-RC, transcript variant C (LIMK1), mRNA
0	0	1	3	2	NM_167326.1	CG1848-RA, transcript variant A (LIMK1), mRNA

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