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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4539R-1 |
| Symbol |
Bka |
Full Name |
Bekka |
| CG No |
CG4539 |
Old CG No |
CG4539 |
| Synonyms |
CG4539, mip, l(2)02695, BcDNA:RE14949, Bka |
| Accession No (Link to NCBI) |
NM_080128.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGACGCGCGGCTTAAGGATCA |
| Primer Seq. 3' |
aatctagaggtaccCGCTCAATCGCGTACTTATGC |
| Predicted Fragment Size |
498 |
| IR fragment full Seq |
0001 AGACGCGCGG CTTAAGGATC AGATTCGAGT GAAAAGAAAG AAGGCCGAGG ATCCGCACCA
0061 GATCAAGGTG CACGAGGCCA CCCAGCAGAG NTCCGCTCTG TTCTTCCAGT ACAACACCCA
0121 GCTGGGACCG CCGTACCACA TCGTTCTGGA CACGAACTTC ATTAACTTTA GCATTAAGAA
0181 CAAACTGGAT ATTGTGCAAG GTATGATGGA CTGTCTGTAT GCCAAGTGCA TACCCTATAT
0241 CTCCGACTGC GTGCGTGCCG AGCTGGAGAA GCTGGGCAAC AAGTACAAGC TGGCCCTGCG
0301 CATCATCTCC GATCCCAGAT TCGAGCGACT GCCGTGCCTG CACAAGGGCA CCTATGCGGA
0361 CGATTGCCTC GTGGAGCGGG TGCGCCAGCA CAAATGCTAC ATTGTGGCCA CCAACGACAA
0421 GGACCTTAAG AATCGCATCC GGAAGATTCC CGGCGTGCCC ATCATGTACG tNGCTGNGCA
0481 TAAGTACGCG ATTGAGCG |
| in silico PCR Fragment |
0001 AGACGCGCGG CTTAAGGATC AGATTCGAGT GAAAAGAAAG AAGGCCGAGG ATCCGCACCA
0061 GATCAAGGTG CACGAGGCCA CCCAGCAGAG CTCCGCTCTG TTCTTCCAGT ACAACACCCA
0121 GCTGGGACCG CCGTACCACA TCGTTCTGGA CACGAACTTC ATTAACTTTA GCATTAAGAA
0181 CAAACTGGAT ATTGTGCAAG GTATGATGGA CTGTCTGTAT GCCAAGTGCA TACCCTATAT
0241 CTCCGACTGC GTGCGTGCCG AGCTGGAGAA GCTGGGCAAC AAGTACAAGC TGGCCCTGCG
0301 CATCATCTCC GATCCCAGAT TCGAGCGACT GCCGTGCCTG CACAAGGGCA CCTATGCGGA
0361 CGATTGCCTC GTGGAGCGGG TGCGCCAGCA CAAATGCTAC ATTGTGGCCA CCAACGACAA
0421 GGACCTTAAG AATCGCATCC GGAAGATTCC CGGCGTGCCC ATCATGTACG TGGCTGCGCA
0481 TAAGTACGCG ATTGAGCG |
| Assemble Data |
4539R-1.IR_full 1 AGACGCGCGGCTTAAGGATCAGATTCGAGTGAAAAGAAAGAAGGCCGAGGATCCGCACCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 1 AGACGCGCGGCTTAAGGATCAGATTCGAGTGAAAAGAAAGAAGGCCGAGGATCCGCACCA 60
4539R-1.IR_full 61 GATCAAGGTGCACGAGGCCACCCAGCAGAGNTCCGCTCTGTTCTTCCAGTACAACACCCA 120
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
4539R-1.in silico 61 GATCAAGGTGCACGAGGCCACCCAGCAGAGCTCCGCTCTGTTCTTCCAGTACAACACCCA 120
4539R-1.IR_full 121 GCTGGGACCGCCGTACCACATCGTTCTGGACACGAACTTCATTAACTTTAGCATTAAGAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 121 GCTGGGACCGCCGTACCACATCGTTCTGGACACGAACTTCATTAACTTTAGCATTAAGAA 180
4539R-1.IR_full 181 CAAACTGGATATTGTGCAAGGTATGATGGACTGTCTGTATGCCAAGTGCATACCCTATAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 181 CAAACTGGATATTGTGCAAGGTATGATGGACTGTCTGTATGCCAAGTGCATACCCTATAT 240
4539R-1.IR_full 241 CTCCGACTGCGTGCGTGCCGAGCTGGAGAAGCTGGGCAACAAGTACAAGCTGGCCCTGCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 241 CTCCGACTGCGTGCGTGCCGAGCTGGAGAAGCTGGGCAACAAGTACAAGCTGGCCCTGCG 300
4539R-1.IR_full 301 CATCATCTCCGATCCCAGATTCGAGCGACTGCCGTGCCTGCACAAGGGCACCTATGCGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 301 CATCATCTCCGATCCCAGATTCGAGCGACTGCCGTGCCTGCACAAGGGCACCTATGCGGA 360
4539R-1.IR_full 361 CGATTGCCTCGTGGAGCGGGTGCGCCAGCACAAATGCTACATTGTGGCCACCAACGACAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4539R-1.in silico 361 CGATTGCCTCGTGGAGCGGGTGCGCCAGCACAAATGCTACATTGTGGCCACCAACGACAA 420
4539R-1.IR_full 421 GGACCTTAAGAATCGCATCCGGAAGATTCCCGGCGTGCCCATCATGTACGTNGCTGNGCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||| |||| |||
4539R-1.in silico 421 GGACCTTAAGAATCGCATCCGGAAGATTCCCGGCGTGCCCATCATGTACGTGGCTGCGCA 480
4539R-1.IR_full 481 TAAGTACGCGATTGAGCG 498
||||||||||||||||||
4539R-1.in silico 481 TAAGTACGCGATTGAGCG 498
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
480 |
0 |
0 |
0 |
NM_080128.2 |
CG4539-RA (Bka), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_136625.2 |
CG8027-RA (CG8027), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_165500.1 |
CG30156-RA (CG30156), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143205.1 |
CG14551-RA (CG14551), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_166223.1 |
CG6556-RA (cnk), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176435.1 |
CG8176-RA, transcript variant A (CG8176), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176436.1 |
CG8176-RB, transcript variant B (CG8176), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079375.2 |
CG5949-RA (DNApol-delta), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_001042819.1 |
CG5424-RF, transcript variant F (f), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_176746.1 |
CG5424-RC, transcript variant C (f), mRNA |
| 0 |
0 |
1 |
4 |
15 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_144045.1 |
CG13551-RA, transcript variant A (CG13551), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166597.1 |
CG13551-RB, transcript variant B (CG13551), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166598.1 |
CG13551-RC, transcript variant C (CG13551), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001032005.1 |
CG6782-RD, transcript variant D (CG6782), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001032008.1 |
CG6783-RC, transcript variant C (CG6783), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001032009.1 |
CG6783-RB, transcript variant B (CG6783), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001032010.1 |
CG6783-RA, transcript variant A (CG6783), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_057518.3 |
CG6358-RA (Xpac), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135746.2 |
CG5792-RA, transcript variant A (CG5792), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165019.2 |
CG5792-RB, transcript variant B (CG5792), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139532.1 |
CG14967-RA (CG14967), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_134591.2 |
CG1501-RA (unc), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_079153.3 |
CG13888-RA (Gr61a), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057760.4 |
CG12245-RA (gcm), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_057922.3 |
CG2699-RA (Pi3K21B), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057770.3 |
CG3193-RA (crn), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_176745.1 |
CG5424-RD, transcript variant D (f), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078660.2 |
CG5424-RB, transcript variant B (f), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169430.2 |
CG6939-RB, transcript variant B (Sbf), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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