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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4524R-1 
 Symbol CG32573  Full Name CG32573 
 CG No CG32573  Old CG No CG4524 
 Synonyms CG4524, CG13013, CG32573 
 Accession No (Link to NCBI) NM_167529.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGATCCAGCTGCTCCAGTTG 
 Primer Seq. 3' 
aatctagaggtaccTCGCGATCCGCGTACAGTTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTGATCCAGC TGCTCCAGTT GCTTCTATGT ACTATTCCAC TCGTTGTGGG CGAGGTTCTG 
0061 CCCCTGCAAC TCGATTTGGA CAACAGTCAA ATTACCATAC GGCGAAGTGC CAGTCGCGAT 
0121 GACAAATTGC AGGTATTCCA CAGCATCTGG GGCGCCATTC GCTCCGACTT CGCCACCGAT 
0181 ACGGACAAGG TGAAGGGCCT GTACAGTCAG CTGGGCGGAT TTATTCGCGA CGATGTGCAG 
0241 TGCAGCAGCG GCTCCACGGG TCGATCTGCC TGCGAGCTGC AGCAGGAGGT GCGACGCCAC 
0301 CTGCGGNACC CTCATGGCCC AGCGGCTGGC CAATCTGTTG AACGCCGAGG AGAGCATTCA 
0361 GACATACGGT ATGTACATAG CGGCCAGTGT GGAGCCCGCC CTGGTCAGGG ACATTCTGGT 
0421 GCACGCCATC GAGGACGTGT ACTTCCATCG ACCGCTGGAG AAGCTGGTGC AGCAGCTGGA 
0481 GCAACTGTAC GcGGATCgCG A 
 in silico PCR Fragment 
0001 ATGATCCAGC TGCTCCAGTT GCTTCTATGT ACTATTCCAC TCGTTGTGGG CGAGGTTCTG 
0061 CCCCTGCAAC TCGATTTGGA CAACAGTCAA ATTACCATAC GGCGAAGTGC CAGTCGCGAT 
0121 GACAAATTGC AGGTATTCCA CAGCATCTGG GGCGCCATTC GCTCCGACTT CGCCACCGAT 
0181 ACGGACAAGG TGAAGGGCCT GTACAGTCAG CTGGGCGGAT ATATTCGCGA CGATGTGCAG 
0241 TGCAGTAGCG GCTCCACGGG TCGATCTGCC TGCGAGCTGC AGCAGGAGGT GCGACGCCAC 
0301 CTGCGG-ACC CTCATGGCCC AGCGGCTGGC CAATCTGTTG AACGCCGAGG AGAGCATTCA 
0361 GACATACGGT ATGTACATAG CGGCCAGTGT GGAGCCCGCC CTGGTCAGGG ACATTCTGGT 
0421 GCACGCCATC GAGGACGTGT ACTTCCATCG ACCGCTGGAG AAGCTGGTGC AGCAGCTGGA 
0481 GCAACTGTAC GCGGATCGCG A 
 Assemble Data 

4524R-1.IR_full        1   TGATCCAGCTGCTCCAGTTGCTTCTATGTACTATTCCACTCGTTGTGGGCGAGGTTCTG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico      1   TGATCCAGCTGCTCCAGTTGCTTCTATGTACTATTCCACTCGTTGTGGGCGAGGTTCTG 59


4524R-1.IR_full       61  CCCCTGCAACTCGATTTGGACAACAGTCAAATTACCATACGGCGAAGTGCCAGTCGCGAT 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     61  CCCCTGCAACTCGATTTGGACAACAGTCAAATTACCATACGGCGAAGTGCCAGTCGCGAT 119


4524R-1.IR_full       121 GACAAATTGCAGGTATTCCACAGCATCTGGGGCGCCATTCGCTCCGACTTCGCCACCGAT 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     121 GACAAATTGCAGGTATTCCACAGCATCTGGGGCGCCATTCGCTCCGACTTCGCCACCGAT 179


4524R-1.IR_full       181 ACGGACAAGGTGAAGGGCCTGTACAGTCAGCTGGGCGGATTTATTCGCGACGATGTGCAG 239
                          |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
4524R-1.in silico     181 ACGGACAAGGTGAAGGGCCTGTACAGTCAGCTGGGCGGATATATTCGCGACGATGTGCAG 239


4524R-1.IR_full       241 TGCAGCAGCGGCTCCACGGGTCGATCTGCCTGCGAGCTGCAGCAGGAGGTGCGACGCCAC 299
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     241 TGCAGTAGCGGCTCCACGGGTCGATCTGCCTGCGAGCTGCAGCAGGAGGTGCGACGCCAC 299


4524R-1.IR_full       301 CTGCGGNACCCTCATGGCCCAGCGGCTGGCCAATCTGTTGAACGCCGAGGAGAGCATTCA 359
                          |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     301 CTGCGG-ACCCTCATGGCCCAGCGGCTGGCCAATCTGTTGAACGCCGAGGAGAGCATTCA 359


4524R-1.IR_full       361 GACATACGGTATGTACATAGCGGCCAGTGTGGAGCCCGCCCTGGTCAGGGACATTCTGGT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     361 GACATACGGTATGTACATAGCGGCCAGTGTGGAGCCCGCCCTGGTCAGGGACATTCTGGT 419


4524R-1.IR_full       421 GCACGCCATCGAGGACGTGTACTTCCATCGACCGCTGGAGAAGCTGGTGCAGCAGCTGGA 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4524R-1.in silico     421 GCACGCCATCGAGGACGTGTACTTCCATCGACCGCTGGAGAAGCTGGTGCAGCAGCTGGA 479


4524R-1.IR_full       481 GCAACTGTACGCGGATCGCGA 500
                          |||||||||||||||||||||
4524R-1.in silico     481 GCAACTGTACGCGGATCGCGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  5  NM_167529.1  CG32573-RA (CG32573), mRNA 
0.2   1  8  2  9  NM_164638.1  CG14025-RC, transcript variant C (Bsg25D), mRNA 
0.2   1  8  2  9  NM_057568.4  CG14025-RA, transcript variant A (Bsg25D), mRNA 
0.2   1  8  2  9  NM_164637.1  CG14025-RB, transcript variant B (Bsg25D), mRNA 
0.2   1  3  3  5  NM_141504.1  CG2747-RA, transcript variant A (CG2747), mRNA 
0.2   1  3  3  2  NM_169205.1  CG2747-RB, transcript variant B (CG2747), mRNA 
0   0  3  11  21  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   0  3  4  9  NM_001042872.1  CG14042-RB, transcript variant B (CG14042), mRNA 
0   0  3  4  9  NM_001042871.1  CG14042-RA, transcript variant A (CG14042), mRNA 
0   0  3  4  9  NM_144359.2  CG14041-RB, transcript variant B (SP555), mRNA 
0   0  3  4  9  NM_164618.1  CG14041-RA, transcript variant A (SP555), mRNA 
0   0  3  2  8  NM_141572.1  CG11768-RA (CG11768), mRNA 
0   0  3  2  8  NM_079673.2  CG14307-RF, transcript variant F (fru), mRNA 
0   0  3  2  8  NM_169816.1  CG14307-RB, transcript variant B (fru), mRNA 
0   0  3  2  3  NM_143555.2  CG9717-RA (CG9717), mRNA 
0   0  2  5  5  NM_080167.3  CG9074-RA (Mst57Da), mRNA 
0   0  2  5  3  NM_001014496.1  CG1506-RC, transcript variant C (Ac3), mRNA 
0   0  2  5  3  NM_165389.1  CG1506-RB, transcript variant B (Ac3), mRNA 
0   0  2  5  3  NM_136272.2  CG1506-RA, transcript variant A (Ac3), mRNA 
0   0  2  4  3  NM_164674.1  CG31643-RA (CG31643), mRNA 
0   0  2  3  13  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  2  3  12  NM_080325.3  CG6450-RC (lva), mRNA 
0   0  2  3  11  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  2  2  8  NM_135954.1  CG13260-RA (CG13260), mRNA 
0   0  2  2  6  NM_143536.1  CG15537-RA (CG15537), mRNA 
0   0  1  12  45  NM_137342.2  CG30460-RC, transcript variant C (CG30460), mRNA 
0   0  1  12  42  NM_206150.1  CG30460-RB, transcript variant B (CG30460), mRNA 
0   0  1  12  42  NM_206149.1  CG30460-RA, transcript variant A (CG30460), mRNA 
0   0  1  6  4  NM_167285.2  CG11727-RA, transcript variant A (CG11727), mRNA 
0   0  1  6  4  NM_132488.3  CG11727-RB, transcript variant B (CG11727), mRNA 

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