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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4486R-1 |
| Symbol |
Cyp9b2 |
Full Name |
Cytochrome P450-9b2 |
| CG No |
CG4486 |
Old CG No |
CG4486 |
| Synonyms |
cyp9b2, 9b2, P450, P-450, BEST:GH08116, CG4486, Cyp9b2 |
| Accession No (Link to NCBI) |
NM_078922.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Dourlen P, Fernandez-Gomez FJ, Dupont C, Grenier-Boley B, Bellenguez C, Obriot H, Caillierez R, Sottejeau Y, Chapuis J, Bretteville A, Abdelfettah F, Delay C, Malmanche N, Soininen H, Hiltunen M, Galas MC, Amouyel P, Sergeant N, Buée L, Lambert JC, Dermaut B.
Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology.
Mol Psychiatry (2017) 22(6) 874-883
[
PubMed ID = 27113998
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTTGTCTGGCCCTTGTGGTCA |
| Primer Seq. 3' |
aatctagaggtaccCTTTCTGCCGGGAAGCGTTTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTTGTCTGGC CCTTGTGGTC ATCGGTTATC TCATCTACAA ATGGAGCACG GCCACCTTCA
0061 AAACCTTTGA GGAAAGGAAG CTCTACTTCG AAAAGCCATA TCCCTTTGTG GGTAATATGG
0121 CTGCAGCGGC CTTGCAGAAG TCGTCCTTCC AGAGACAACT CACCGAGTTC TACGAACGCA
0181 CTCGTCAACA CAAACTGGTG GGCTTCTTCA ATATGCGCAC ACCTATGATC ACGCTAAACG
0241 ACCCGGAGCT GATCAAAAAG GTCTGCGTAA AGGACTTTGA CCACTTCCCC AACCACCAGC
0301 CCTTCATCAC CTCCAACGAT CGCCTGTTCA ACGACATGCT CAGCGTGATG AGGGACCAGC
0361 GGTGGAAGCA CATGAGAAAC ACCCTCACTC CGGTCTTCAC GGCGGCCAAA ATGCGCAACA
0421 TGTTCACCCT GATGAACGAG AGCTTCGCGG AGTGCCTGCA GCACCTGGAC AGCTCTTCGA
0481 AAACGCTTCC CGGCAGAAAG |
| in silico PCR Fragment |
0001 TTTGTCTGGC CCTTGTGGTC ATCGGTTATC TCATCTACAA ATGGAGCACG GCCACCTTCA
0061 AAACCTTTGA GGAAAGGAAG CTCTACTTCG AAAAGCCATA TCCCTTTGTG GGTAATATGG
0121 CTGCAGCGGC CTTGCAGAAG TCGTCCTTCC AGAGACAACT CACCGAGTTC TACGAACGCA
0181 CTCGTCAACA CAAACTGGTG GGCTTCTTCA ATATGCGCAC ACCTATGATC ACGCTAAACG
0241 ACCCGGAGCT GATCAAAAAG GTCTGCGTAA AGGACTTTGA CCACTTCCCC AACCACCAGC
0301 CCTTCATCAC CTCCAACGAT CGCCTGTTCA ACGACATGCT CAGCGTGATG AGGGACCAGC
0361 GGTGGAAGCA CATGAGAAAC ACCCTCACTC CGGTCTTCAC GGCGGCCAAA ATGCGCAACA
0421 TGTTCACCCT GATGAACGAG AGCTTCGCGG AGTGCCTGCA GCACCTGGAC AGCTCTTCGA
0481 AAACGCTTCC CGGCAGAAAG |
| Assemble Data |
4486R-1.IR_full 1 TTTGTCTGGCCCTTGTGGTCATCGGTTATCTCATCTACAAATGGAGCACGGCCACCTTCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 1 TTTGTCTGGCCCTTGTGGTCATCGGTTATCTCATCTACAAATGGAGCACGGCCACCTTCA 60
4486R-1.IR_full 61 AAACCTTTGAGGAAAGGAAGCTCTACTTCGAAAAGCCATATCCCTTTGTGGGTAATATGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 61 AAACCTTTGAGGAAAGGAAGCTCTACTTCGAAAAGCCATATCCCTTTGTGGGTAATATGG 120
4486R-1.IR_full 121 CTGCAGCGGCCTTGCAGAAGTCGTCCTTCCAGAGACAACTCACCGAGTTCTACGAACGCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 121 CTGCAGCGGCCTTGCAGAAGTCGTCCTTCCAGAGACAACTCACCGAGTTCTACGAACGCA 180
4486R-1.IR_full 181 CTCGTCAACACAAACTGGTGGGCTTCTTCAATATGCGCACACCTATGATCACGCTAAACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 181 CTCGTCAACACAAACTGGTGGGCTTCTTCAATATGCGCACACCTATGATCACGCTAAACG 240
4486R-1.IR_full 241 ACCCGGAGCTGATCAAAAAGGTCTGCGTAAAGGACTTTGACCACTTCCCCAACCACCAGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 241 ACCCGGAGCTGATCAAAAAGGTCTGCGTAAAGGACTTTGACCACTTCCCCAACCACCAGC 300
4486R-1.IR_full 301 CCTTCATCACCTCCAACGATCGCCTGTTCAACGACATGCTCAGCGTGATGAGGGACCAGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 301 CCTTCATCACCTCCAACGATCGCCTGTTCAACGACATGCTCAGCGTGATGAGGGACCAGC 360
4486R-1.IR_full 361 GGTGGAAGCACATGAGAAACACCCTCACTCCGGTCTTCACGGCGGCCAAAATGCGCAACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 361 GGTGGAAGCACATGAGAAACACCCTCACTCCGGTCTTCACGGCGGCCAAAATGCGCAACA 420
4486R-1.IR_full 421 TGTTCACCCTGATGAACGAGAGCTTCGCGGAGTGCCTGCAGCACCTGGACAGCTCTTCGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4486R-1.in silico 421 TGTTCACCCTGATGAACGAGAGCTTCGCGGAGTGCCTGCAGCACCTGGACAGCTCTTCGA 480
4486R-1.IR_full 481 AAACGCTTCCCGGCAGAAAG 500
||||||||||||||||||||
4486R-1.in silico 481 AAACGCTTCCCGGCAGAAAG 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_078922.2 |
CG4486-RA (Cyp9b2), mRNA |
| 2.9 |
14 |
28 |
48 |
72 |
NM_078921.2 |
CG4485-RA (Cyp9b1), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_165964.1 |
CG30487-RA (CG30487), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_141880.2 |
CG10005-RA (CG10005), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_079806.2 |
CG31062-RA (side), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132050.1 |
CG3011-RA (CG3011), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143702.2 |
CG5889-RA (Mdh), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_137420.2 |
CG5009-RA (CG5009), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_140184.2 |
CG7628-RA (CG7628), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141130.3 |
CG11489-RB, transcript variant B (CG11489), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_176386.1 |
CG11489-RC, transcript variant C (CG11489), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_057523.3 |
CG18412-RA (ph-p), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_080055.2 |
CG3895-RA (ph-d), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166093.1 |
CG8174-RA, transcript variant A (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137190.2 |
CG8174-RB, transcript variant B (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166094.1 |
CG8174-RC, transcript variant C (SRPK), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_140780.1 |
CG13700-RA (CG13700), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_134869.2 |
CG2964-RA (CG2964), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141993.2 |
CG7518-RB, transcript variant B (CG7518), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169489.1 |
CG7518-RA, transcript variant A (CG7518), mRNA |
| 0 |
0 |
0 |
8 |
7 |
NM_136727.2 |
CG18408-RA, transcript variant A (CAP), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_079737.2 |
CG17077-RB, transcript variant B (pnt), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_170070.1 |
CG17077-RC, transcript variant C (pnt), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_170651.3 |
CG17077-RD, transcript variant D (pnt), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_136647.2 |
CG8809-RA (Camta), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_139684.2 |
CG17030-RA (CG17030), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_138003.2 |
CG5591-RA (CG5591), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_135201.1 |
CG9595-RA (osm-6), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168879.2 |
CG9936-RC, transcript variant C (skd), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_079914.2 |
CG9936-RD, transcript variant D (skd), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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