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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4479R-1 |
| Symbol |
Mst35Ba |
Full Name |
Male-specific-transcript-35Ba |
| CG No |
CG4479 |
Old CG No |
CG4479 |
| Synonyms |
mst35Bb, BG:DS03431.2, mst35b, CG4479, BcDNA:AT31403, Mst35Ba |
| Accession No (Link to NCBI) |
NM_057702.4 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTAAATGAGTGCAAGAGCCTG |
| Primer Seq. 3' |
aatctagaggtaccGTATTGCTGGCAAATCCGTCG |
| Predicted Fragment Size |
423 |
| IR fragment full Seq |
0001 GTAAATGAGT GCAAGAGCCT GTGGAATGGC ATAATTTCCA TTTCTGCAAA AGATGAAAGT
0061 CCTAAAGGTC TCACTGAAAT GTGTAATCAT CCAATAAGGA GAGCACCTCA AAAATGTAAG
0121 CCAATGAAGT CCTGTGCAAA GCCGCGCCGA AAGGCAGCCT GTGCCAAGGC GACTCGGCCC
0181 AAGGTCAAGT GTGCACCGAG GCAGAAGTGC AGCAAGCAGG GACCTGTCAC TAACAACGCC
0241 TATTTGAATT TTGTGCGTTC CTTCCGAAAG AAGCACTGTA ACTTGAAACC GCGGGAATTA
0301 ATTGCAAAGG CCGCTAAAGC GTGGGCCAGG CTCTCGGAGA ATAGAAAGGA TCGATACCGC
0361 CGGATGGCAT GCAAGGTCAC CACCAGTGAA CGCCACAAGC GCCGACGGAT TTGCCAGCAA
0421 TAC |
| in silico PCR Fragment |
0001 GTAAATGAGT GCAAGAGCCT GTGGAATGGC ATAATTTCCA TTTCTGCAAA AGATGAAAGT
0061 CCTAAAGGTC TCACTGAAAT GTGTAATCAT CCAATAAGGA GAGCACCTCA AAAATGTAAG
0121 CCAATGAAGT CCTGTGCAAA GCCGCGCCGA AAGGCAGCCT GTGCCAAGGC GACTCGGCCC
0181 AAGGTCAAGT GTGCACCGAG GCAGAAGTGC AGCAAGCAGG GACCTGTCAC TAACAACGCC
0241 TATTTGAATT TTGTGCGTTC CTTCCGAAAG AAGCACTGTA ACTTGAAACC GCGGGAATTA
0301 ATTGCAAAGG CCGCTAAAGC GTGGGCCAGG CTCTCGGAGA ATAGAAAGGA TCGATACCGC
0361 CGGATGGCAT GCAAGGTCAC CACCAGTGAA CGCCACAAGC GCCGACGGAT TTGCCAGCAA
0421 TAC |
| Assemble Data |
4479R-1.IR_full 1 GTAAATGAGTGCAAGAGCCTGTGGAATGGCATAATTTCCATTTCTGCAAAAGATGAAAGT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 1 GTAAATGAGTGCAAGAGCCTGTGGAATGGCATAATTTCCATTTCTGCAAAAGATGAAAGT 60
4479R-1.IR_full 61 CCTAAAGGTCTCACTGAAATGTGTAATCATCCAATAAGGAGAGCACCTCAAAAATGTAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 61 CCTAAAGGTCTCACTGAAATGTGTAATCATCCAATAAGGAGAGCACCTCAAAAATGTAAG 120
4479R-1.IR_full 121 CCAATGAAGTCCTGTGCAAAGCCGCGCCGAAAGGCAGCCTGTGCCAAGGCGACTCGGCCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 121 CCAATGAAGTCCTGTGCAAAGCCGCGCCGAAAGGCAGCCTGTGCCAAGGCGACTCGGCCC 180
4479R-1.IR_full 181 AAGGTCAAGTGTGCACCGAGGCAGAAGTGCAGCAAGCAGGGACCTGTCACTAACAACGCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 181 AAGGTCAAGTGTGCACCGAGGCAGAAGTGCAGCAAGCAGGGACCTGTCACTAACAACGCC 240
4479R-1.IR_full 241 TATTTGAATTTTGTGCGTTCCTTCCGAAAGAAGCACTGTAACTTGAAACCGCGGGAATTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 241 TATTTGAATTTTGTGCGTTCCTTCCGAAAGAAGCACTGTAACTTGAAACCGCGGGAATTA 300
4479R-1.IR_full 301 ATTGCAAAGGCCGCTAAAGCGTGGGCCAGGCTCTCGGAGAATAGAAAGGATCGATACCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 301 ATTGCAAAGGCCGCTAAAGCGTGGGCCAGGCTCTCGGAGAATAGAAAGGATCGATACCGC 360
4479R-1.IR_full 361 CGGATGGCATGCAAGGTCACCACCAGTGAACGCCACAAGCGCCGACGGATTTGCCAGCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4479R-1.in silico 361 CGGATGGCATGCAAGGTCACCACCAGTGAACGCCACAAGCGCCGACGGATTTGCCAGCAA 420
4479R-1.IR_full 421 TAC 423
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4479R-1.in silico 421 TAC 423
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
405 |
0 |
0 |
0 |
NM_057702.4 |
CG4479-RA (Mst35Ba), mRNA |
| 55.8 |
226 |
75 |
64 |
28 |
NM_057703.3 |
CG4478-RA (Mst35Bb), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_135967.1 |
CG13272-RA (CG13272), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_136446.2 |
CG1603-RA (CG1603), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_080330.2 |
CG3620-RA, transcript variant A (norpA), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001014721.1 |
CG3620-RC, transcript variant C (norpA), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001014720.1 |
CG3620-RD, transcript variant D (norpA), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_167008.1 |
CG3620-RB, transcript variant B (norpA), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_142783.2 |
CG13849-RA (Nop56), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142734.1 |
CG6656-RA (CG6656), mRNA |
| 0 |
0 |
0 |
8 |
9 |
NM_132130.1 |
CG12796-RA (CG12796), mRNA |
| 0 |
0 |
0 |
8 |
3 |
NM_137084.2 |
CG8241-RA (CG8241), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_079056.2 |
CG5784-RB, transcript variant B (Mapmodulin), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166258.1 |
CG5784-RA, transcript variant A (Mapmodulin), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_001043085.1 |
CG34123-RC, transcript variant C (CG34123), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_001043083.1 |
CG34123-RB, transcript variant B (CG34123), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_001043084.1 |
CG34123-RD, transcript variant D (CG34123), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167646.1 |
CG32827-RA (CG32827), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_139366.2 |
CG12099-RB, transcript variant B (CG12099), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167895.1 |
CG12099-RA, transcript variant A (CG12099), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001014552.1 |
CG15792-RD, transcript variant D (zip), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079136.2 |
CG15792-RA, transcript variant A (zip), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001014553.1 |
CG15792-RC, transcript variant C (zip), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166704.1 |
CG15792-RB, transcript variant B (zip), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_057506.2 |
CG7672-RA, transcript variant A (gl), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169807.2 |
CG7672-RB, transcript variant B (gl), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_080306.2 |
CG3443-RB (pcx), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_206140.1 |
CG8912-RC, transcript variant C (Psi), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_057775.3 |
CG8912-RB, transcript variant B (Psi), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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