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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4466R-1 |
| Symbol |
Hsp27 |
Full Name |
Heat shock protein 27 |
| CG No |
CG4466 |
Old CG No |
CG4466 |
| Synonyms |
hsp27, Hsp28, HSP28, 27K, DmHsp27, hsp-27, HSP27, hsp28, Dhsp27, hsp26, hsp27/26, 28, hsp 27, CG4466, anon-WO0140519.69, Hsp27 |
| Accession No (Link to NCBI) |
NM_079276.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTATACCACTGCTGCACTTGG |
| Primer Seq. 3' |
aatctagaggtaccCCTTGAGGGTCAGCACACCGT |
| Predicted Fragment Size |
477 |
| IR fragment full Seq |
0001 TTATACCACT GCTGCACTTG GCCCGGGAGT TGGATCATGA CTACCGCACC GACTGGGGGC
0061 ATTTGCTGGA GGATGACTTC GGTTTTGGCG TCCATGCCCA CGATCTGTTC CATCCGCGTC
0121 GCCTGCTACT GCCCAACACC CTGGGACTGG GTCGTCGTCG TTATTCGCCG TACGAGAGGA
0181 GCCATGGCCA CCACAATCAA ATGTCACGTC GCGCGTCGGG GGGTCCAAAC GCTCTGCTGC
0241 CCGCCGTGGG CAAAGATGGC TTCCAGGTGT GCATGGATGT GTCGCAGTTC AAGCCCAACG
0301 AGCTGACCGT CAAGGTGGTG GACAACACCG TGGTGGTAGA GGGGAAGCAC GAGGAGCGCG
0361 AGGACGGCCA TGGAATGATC CAGCGTCACT TTGTGCGCAA GTATACCCTG CCCAAGGGCT
0421 TTGACCCCAA CGAGGTAGTG TCCACTGTCT CATCCGACGG TGTGCTGACC CTCAAGG |
| in silico PCR Fragment |
0001 TTATACCACT GCTGCACTTG GCCCGGGAGT TGGATCATGA CTACCGCACC GACTGGGGGC
0061 ATTTGCTGGA GGATGACTTC GGTTTTGGCG TCCATGCCCA CGATCTGTTC CATCCGCGTC
0121 GCCTGCTACT GCCCAACACC CTGGGACTGG GTCGTCGTCG TTATTCGCCG TACGAGAGGA
0181 GCCATGGCCA CCACAATCAA ATGTCACGTC GCGCGTCGGG GGGTCCAAAC GCTCTGCTGC
0241 CCGCCGTGGG CAAAGATGGC TTCCAGGTGT GCATGGATGT GTCGCAGTTC AAGCCCAACG
0301 AGCTGACCGT CAAGGTGGTG GACAACACCG TGGTGGTAGA GGGGAAGCAC GAGGAGCGCG
0361 AGGACGGCCA TGGAATGATC CAGCGTCACT TTGTGCGCAA GTATACCCTG CCCAAGGGCT
0421 TTGACCCCAA CGAGGTAGTG TCCACTGTCT CATCCGACGG TGTGCTGACC CTCAAGG |
| Assemble Data |
4466R-1.IR_full 1 TTATACCACTGCTGCACTTGGCCCGGGAGTTGGATCATGACTACCGCACCGACTGGGGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 1 TTATACCACTGCTGCACTTGGCCCGGGAGTTGGATCATGACTACCGCACCGACTGGGGGC 60
4466R-1.IR_full 61 ATTTGCTGGAGGATGACTTCGGTTTTGGCGTCCATGCCCACGATCTGTTCCATCCGCGTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 61 ATTTGCTGGAGGATGACTTCGGTTTTGGCGTCCATGCCCACGATCTGTTCCATCCGCGTC 120
4466R-1.IR_full 121 GCCTGCTACTGCCCAACACCCTGGGACTGGGTCGTCGTCGTTATTCGCCGTACGAGAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 121 GCCTGCTACTGCCCAACACCCTGGGACTGGGTCGTCGTCGTTATTCGCCGTACGAGAGGA 180
4466R-1.IR_full 181 GCCATGGCCACCACAATCAAATGTCACGTCGCGCGTCGGGGGGTCCAAACGCTCTGCTGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 181 GCCATGGCCACCACAATCAAATGTCACGTCGCGCGTCGGGGGGTCCAAACGCTCTGCTGC 240
4466R-1.IR_full 241 CCGCCGTGGGCAAAGATGGCTTCCAGGTGTGCATGGATGTGTCGCAGTTCAAGCCCAACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 241 CCGCCGTGGGCAAAGATGGCTTCCAGGTGTGCATGGATGTGTCGCAGTTCAAGCCCAACG 300
4466R-1.IR_full 301 AGCTGACCGTCAAGGTGGTGGACAACACCGTGGTGGTAGAGGGGAAGCACGAGGAGCGCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 301 AGCTGACCGTCAAGGTGGTGGACAACACCGTGGTGGTAGAGGGGAAGCACGAGGAGCGCG 360
4466R-1.IR_full 361 AGGACGGCCATGGAATGATCCAGCGTCACTTTGTGCGCAAGTATACCCTGCCCAAGGGCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 361 AGGACGGCCATGGAATGATCCAGCGTCACTTTGTGCGCAAGTATACCCTGCCCAAGGGCT 420
4466R-1.IR_full 421 TTGACCCCAACGAGGTAGTGTCCACTGTCTCATCCGACGGTGTGCTGACCCTCAAGG 477
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4466R-1.in silico 421 TTGACCCCAACGAGGTAGTGTCCACTGTCTCATCCGACGGTGTGCTGACCCTCAAGG 477
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
459 |
0 |
0 |
0 |
NM_079276.2 |
CG4466-RA (Hsp27), mRNA |
| 0.21 |
1 |
4 |
13 |
9 |
NM_079270.1 |
CG4190-RA (Hsp67Bc), mRNA |
| 0 |
0 |
24 |
11 |
30 |
NM_079275.2 |
CG4463-RA (Hsp23), mRNA |
| 0 |
0 |
7 |
14 |
40 |
NM_079273.2 |
CG4183-RA (Hsp26), mRNA |
| 0 |
0 |
2 |
31 |
13 |
NM_079274.2 |
CG4167-RA (Hsp67Ba), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057685.3 |
CG11527-RA (Tig), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_170078.1 |
CG10175-RB, transcript variant B (CG10175), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_170077.1 |
CG10175-RA, transcript variant A (CG10175), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_142894.1 |
CG10175-RC, transcript variant C (CG10175), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_134962.2 |
CG31772-RA (CG31772), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_057518.3 |
CG6358-RA (Xpac), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079484.4 |
CG7437-RA, transcript variant A (mub), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168946.3 |
CG7437-RB, transcript variant B (mub), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206423.2 |
CG7437-RC, transcript variant C (mub), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001043154.1 |
CG7437-RD, transcript variant D (mub), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132955.1 |
CG4937-RA, transcript variant A (RhoGAP15B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042817.1 |
CG4937-RB, transcript variant B (RhoGAP15B), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132606.1 |
CG3754-RA (CG3754), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137594.2 |
CG11018-RA (CG11018), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_140177.1 |
CG6272-RA (CG6272), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137331.2 |
CG9000-RA (CG9000), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_134690.2 |
CG2807-RA (CG2807), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_079103.2 |
CG4533-RA, transcript variant A (l(2)efl), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_166620.1 |
CG4533-RB, transcript variant B (l(2)efl), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_001014513.1 |
CG8739-RC, transcript variant C (cmp44E), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_165624.1 |
CG8739-RB, transcript variant B (cmp44E), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_165625.1 |
CG8739-RA, transcript variant A (cmp44E), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_079034.2 |
CG8425-RA (Jhe), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168171.1 |
CG32397-RA (CG32397), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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