NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4462R-1

Symbol CG4462 Full Name CG4462

CG No CG4462 Old CG No CG4462

Synonyms CG4462

Accession No (Link to NCBI) NM_142592.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAACTTTATCCCGGCTCGGAG

Primer Seq. 3'

aatctagaggtaccCTGGCAACAGAATCACACCAA

Predicted Fragment Size

IR fragment full Seq

0001 GAACTTTATC CCGGCTCGGA GTTCACCTGC GACACAAGTT ATTTGTCTAG CAGCAGCAAC 
0061 TCCAGTTTTA GTATTTCGGA TAACCAGTGC TATGTCCTGG ACGAGTCCAC TGGCACCAGC 
0121 TCCGAGTGCG AGCAGTTCAA CTACGTATCC AGTTTCGACT CCCTGATCAT GCAGTTCAAT 
0181 CTGGTCTGCT TGAGGGACAT CTTCGTGGCA TGGACTCAGT ACTGGCATTT GTTCGGAGTT 
0241 CTTGTAGGCG GCGTTATGGG CACCAAGATG ATGCTGGGCA TCAGCCCGAG GAGCACCTAT 
0301 TGTGTGGGCG CTGTAGCCCA GATTTTGTGT GGAGTAGTCA CTGGATATGC TAGGGATTTT 
0361 AGTCTGCACT GCGCCTTCCG TTGCCTCTCC GCTGTCTGTT GTGCGATCAT GTTCACAGCT 
0421 GGTCAAGCCA TATTTGCCGA TATCACGGCG GGAATGCATC GCATTGGTGC CATTATCCTA 
0481 TACGACACTT TCTGGTCCAT TGGTGTGATT CTGTTGCCAG

in silico PCR Fragment

0001 GAACTTTATC CCGGCTCGGA GTTCACCTGC GACACCAGTT ATTTGACTAG CAGCAGCAAC 
0061 TCCAGTTTTA GTATTTCGGA TAACCAGTGC TATGTCCTGG ACGAGTCCAC TGGCACCAGC 
0121 TCCGAGTGCG AGCAGTTCAA CTACGTATCC AGTTTCGACT CCCTGATCAT GCAGTTCAAT 
0181 CTGGTCTGCT TGAGGGACAT CTTCGTGGCC TGGACTCAGT ACTGGCATTT GTTCGGAGTT 
0241 CTTGTAGGCG GCGTTATGGG CACCAAGATG ATGCTGGGCA TCAGCCCGAG GAGCACCTAT 
0301 TGTGTCGGTG CTGTAGCCCA GATTTTGTGT GGAGTAGTCA CTGGATATGC TAGGGATTTT 
0361 AGTCTGCACT GCGCCTTCCG TTGTCTCTCC GCTGTCTGTT GTGCGATCAT GTTCACAGCT 
0421 GGTCAAGCCA TATTTGCCGA TATCACGGCG GGAATGCATC GCATTGGTGC CATTATCCTA 
0481 TACGACACTT TCTGGTCCAT TGGTGTGATT CTGTTGCCAG

Assemble Data


4462R-1.IR_full       1   GAACTTTATCCCGGCTCGGAGTTCACCTGCGACACAAGTTATTTGTCTAGCAGCAGCAAC 60
                          ||||||||||||||||||||||||||||||||||| ||||||||| ||||||||||||||
4462R-1.in silico     1   GAACTTTATCCCGGCTCGGAGTTCACCTGCGACACCAGTTATTTGACTAGCAGCAGCAAC 60


4462R-1.IR_full       61  TCCAGTTTTAGTATTTCGGATAACCAGTGCTATGTCCTGGACGAGTCCACTGGCACCAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     61  TCCAGTTTTAGTATTTCGGATAACCAGTGCTATGTCCTGGACGAGTCCACTGGCACCAGC 120


4462R-1.IR_full       121 TCCGAGTGCGAGCAGTTCAACTACGTATCCAGTTTCGACTCCCTGATCATGCAGTTCAAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     121 TCCGAGTGCGAGCAGTTCAACTACGTATCCAGTTTCGACTCCCTGATCATGCAGTTCAAT 180


4462R-1.IR_full       181 CTGGTCTGCTTGAGGGACATCTTCGTGGCATGGACTCAGTACTGGCATTTGTTCGGAGTT 240
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
4462R-1.in silico     181 CTGGTCTGCTTGAGGGACATCTTCGTGGCCTGGACTCAGTACTGGCATTTGTTCGGAGTT 240


4462R-1.IR_full       241 CTTGTAGGCGGCGTTATGGGCACCAAGATGATGCTGGGCATCAGCCCGAGGAGCACCTAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     241 CTTGTAGGCGGCGTTATGGGCACCAAGATGATGCTGGGCATCAGCCCGAGGAGCACCTAT 300


4462R-1.IR_full       301 TGTGTGGGCGCTGTAGCCCAGATTTTGTGTGGAGTAGTCACTGGATATGCTAGGGATTTT 360
                          ||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     301 TGTGTCGGTGCTGTAGCCCAGATTTTGTGTGGAGTAGTCACTGGATATGCTAGGGATTTT 360


4462R-1.IR_full       361 AGTCTGCACTGCGCCTTCCGTTGCCTCTCCGCTGTCTGTTGTGCGATCATGTTCACAGCT 420
                          ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
4462R-1.in silico     361 AGTCTGCACTGCGCCTTCCGTTGTCTCTCCGCTGTCTGTTGTGCGATCATGTTCACAGCT 420


4462R-1.IR_full       421 GGTCAAGCCATATTTGCCGATATCACGGCGGGAATGCATCGCATTGGTGCCATTATCCTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     421 GGTCAAGCCATATTTGCCGATATCACGGCGGGAATGCATCGCATTGGTGCCATTATCCTA 480


4462R-1.IR_full       481 TACGACACTTTCTGGTCCATTGGTGTGATTCTGTTGCCAG 520
                          ||||||||||||||||||||||||||||||||||||||||
4462R-1.in silico     481 TACGACACTTTCTGGTCCATTGGTGTGATTCTGTTGCCAG 520

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	502	0	0	1	NM_142592.1	CG4462-RA (CG4462), mRNA
0.19	1	2	4	8	NM_176746.1	CG5424-RC, transcript variant C (f), mRNA
0.19	1	2	4	8	NM_176745.1	CG5424-RD, transcript variant D (f), mRNA
0.19	1	2	4	8	NM_001042819.1	CG5424-RF, transcript variant F (f), mRNA
0.19	1	2	4	8	NM_078660.2	CG5424-RB, transcript variant B (f), mRNA
0.19	1	2	3	2	NM_167563.2	CG5424-RA, transcript variant A (f), mRNA
0.19	1	2	3	2	NM_176747.1	CG5424-RE, transcript variant E (f), mRNA
0	0	5	10	21	NM_142590.1	CG4465-RA (CG4465), mRNA
0	0	2	9	12	NM_169782.1	CG31243-RA, transcript variant A (cpo), mRNA
0	0	2	9	12	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
0	0	2	9	12	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
0	0	2	7	6	NM_135979.1	CG15134-RA (CG15134), mRNA
0	0	1	5	7	NM_079409.2	CG8127-RA, transcript variant A (Eip75B), mRNA
0	0	1	4	5	NM_136277.2	CG1416-RA, transcript variant A (CG1416), mRNA
0	0	1	4	5	NM_165398.1	CG1416-RB, transcript variant B (CG1416), mRNA
0	0	1	4	5	NM_165399.1	CG1416-RC, transcript variant C (CG1416), mRNA
0	0	1	4	2	NM_136263.1	CG8665-RA (CG8665), mRNA
0	0	1	3	3	NM_132748.1	CG12539-RA (CG12539), mRNA
0	0	1	3	2	NM_139767.1	CG13293-RA (CG13293), mRNA
0	0	1	2	7	NM_001015268.1	CG40450-PB.3 (CG40450), mRNA
0	0	1	2	5	NM_140907.1	CG17732-RA (CG17732), mRNA
0	0	1	2	5	NM_001031886.1	CG33692-RA, transcript variant A (CG33692), mRNA
0	0	1	2	5	NM_001031889.1	CG33691-RA, transcript variant A (CG33691), mRNA
0	0	1	2	5	NM_001031887.1	CG33691-RC, transcript variant C (CG33691), mRNA
0	0	1	2	3	NM_141918.1	CG10013-RA (CG10013), mRNA
0	0	0	7	2	NM_130616.2	CG4290-RA (CG4290), mRNA
0	0	0	5	8	NM_135605.2	CG6729-RA (CG6729), mRNA
0	0	0	5	7	NM_079393.2	CG4083-RA (Mo25), mRNA
0	0	0	5	7	NM_164780.1	CG7392-RC, transcript variant C (Cka), mRNA
0	0	0	5	7	NM_164781.1	CG7392-RD, transcript variant D (Cka), mRNA

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