NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4461R-3

Symbol CG4461 Full Name CG4461

CG No CG4461 Old CG No CG4461

Synonyms 148460_at, Hsp20, gh12586, BEST:GH12586, CG4461

Accession No (Link to NCBI) NM_140047.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTCTCTGGTGCCTACCACATA

Primer Seq. 3'

aatctagaggtaccTGAGGATGCCATCGCTGGATA

Predicted Fragment Size

IR fragment full Seq

0001 GTCTCTGGTG CCTACCACAT ATCGCGATTT GTCGCGCGAA TTTGATCGAC CCCGTCCGCT 
0061 CTACCATCCG CCGTATGATT TCCAGCTATA TCCGTATCTG TGGGACGATT CGCGTCTCTG 
0121 GTGGCCATCT CCTCACACCA GCAGAAGTGA CCTACTCCGA CCTCTGGATG AGCTGGTGTC 
0181 GCGTCGAGTG CGCAACCAGT TGATCCAATC GACGCCGTAT GAGTGGGCGC ATCCAATGAG 
0241 ATGGGACAAC TACTATTCCG GTGAGCGTGT CCATGTGGAT GAGAAGGGAT TTCGAATTGA 
0301 CATCGATGTG CGACAATTTC ATCCGCACGA CATTGTGGTC AAAACCAATG ACGACTATGT 
0361 CATCGTCCAG GGAAATCACA ATCGTCGCGA CGAGGGTTCC AATGGCCTGG TGGAGCGGCA 
0421 CTTTGTGAGG AAGTACCTTC TGCCCCGCGG ATACAATGCC AATGAGGTAA TCTCGGACAT 
0481 ATCCAGCGAT GGCATCCTCA

in silico PCR Fragment

0001 GTCTCTGGTG CCTACCACAT ATCGCGATTT GTCGCGCGAA TTTGATCGAC TCCGTCCGCT 
0061 CTACCATCCG CCGTATGATT TCCAGCTATA TCCGTATCTG TGGGACGATT CGCGTCTCTG 
0121 GTGGCCATCG CCTCACACCA GCAGAAGTGA CCTACTCCGA CCTCTGGATG AGCTGGTGTC 
0181 GCGTCGAGTG CGCAACCAGT TGATCCAATC GACGCCGTAT GAGTGGGCGC ATCCAATGAG 
0241 GTGGGACAAC TACTATTCCG GTGAGCGTGT CCATGTGGAT GAGAAGGGAT TTCGGATTGA 
0301 CATCGATGTG CGACAATTTC ATCCCCACGA CATTGTGGTC AAAACCAATG ACGACTATGT 
0361 CATCGTCCAG GGAAATCACA ATCGTCGCGA CGAGGGTTCC AATGGCCTGG TGGAGCGGCA 
0421 CTTTGTGAGG AAGTACCTTC TGCCCCGCGG ATACAATGCC AATGAGGTAA TCTCGGACAT 
0481 ATCCAGCGAT GGCATCCTCA

Assemble Data


4461R-3.IR_full       1   GTCTCTGGTGCCTACCACATATCGCGATTTGTCGCGCGAATTTGATCGACCCCGTCCGCT 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
4461R-3.in silico     1   GTCTCTGGTGCCTACCACATATCGCGATTTGTCGCGCGAATTTGATCGACTCCGTCCGCT 60


4461R-3.IR_full       61  CTACCATCCGCCGTATGATTTCCAGCTATATCCGTATCTGTGGGACGATTCGCGTCTCTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4461R-3.in silico     61  CTACCATCCGCCGTATGATTTCCAGCTATATCCGTATCTGTGGGACGATTCGCGTCTCTG 120


4461R-3.IR_full       121 GTGGCCATCTCCTCACACCAGCAGAAGTGACCTACTCCGACCTCTGGATGAGCTGGTGTC 180
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
4461R-3.in silico     121 GTGGCCATCGCCTCACACCAGCAGAAGTGACCTACTCCGACCTCTGGATGAGCTGGTGTC 180


4461R-3.IR_full       181 GCGTCGAGTGCGCAACCAGTTGATCCAATCGACGCCGTATGAGTGGGCGCATCCAATGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4461R-3.in silico     181 GCGTCGAGTGCGCAACCAGTTGATCCAATCGACGCCGTATGAGTGGGCGCATCCAATGAG 240


4461R-3.IR_full       241 ATGGGACAACTACTATTCCGGTGAGCGTGTCCATGTGGATGAGAAGGGATTTCGAATTGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
4461R-3.in silico     241 GTGGGACAACTACTATTCCGGTGAGCGTGTCCATGTGGATGAGAAGGGATTTCGGATTGA 300


4461R-3.IR_full       301 CATCGATGTGCGACAATTTCATCCGCACGACATTGTGGTCAAAACCAATGACGACTATGT 360
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
4461R-3.in silico     301 CATCGATGTGCGACAATTTCATCCCCACGACATTGTGGTCAAAACCAATGACGACTATGT 360


4461R-3.IR_full       361 CATCGTCCAGGGAAATCACAATCGTCGCGACGAGGGTTCCAATGGCCTGGTGGAGCGGCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4461R-3.in silico     361 CATCGTCCAGGGAAATCACAATCGTCGCGACGAGGGTTCCAATGGCCTGGTGGAGCGGCA 420


4461R-3.IR_full       421 CTTTGTGAGGAAGTACCTTCTGCCCCGCGGATACAATGCCAATGAGGTAATCTCGGACAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4461R-3.in silico     421 CTTTGTGAGGAAGTACCTTCTGCCCCGCGGATACAATGCCAATGAGGTAATCTCGGACAT 480


4461R-3.IR_full       481 ATCCAGCGATGGCATCCTCA 500
                          ||||||||||||||||||||
4461R-3.in silico     481 ATCCAGCGATGGCATCCTCA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140047.1	CG4461-RA (CG4461), mRNA
0	0	3	4	2	NM_168097.1	CG32247-RA (CG32247), mRNA
0	0	2	4	10	NM_079268.2	CG10923-RA (Klp67A), mRNA
0	0	2	3	2	NM_001014740.1	CG11111-RD, transcript variant D (rdgB), mRNA
0	0	2	3	2	NM_167382.1	CG11111-RA, transcript variant A (rdgB), mRNA
0	0	2	3	2	NM_001014741.1	CG11111-RC, transcript variant C (rdgB), mRNA
0	0	1	4	6	NM_142652.2	CG31200-RA (CG31200), mRNA
0	0	1	4	2	NM_057624.2	CG4698-RA (Wnt4), mRNA
0	0	1	3	4	NM_141666.2	CG9492-RA (CG9492), mRNA
0	0	1	3	3	NM_206391.1	CG33256-RA (CG33256), mRNA
0	0	1	2	4	NM_176156.1	CG30044-RA (CG30044), mRNA
0	0	1	2	2	NM_134988.1	CG15432-RA (CG15432), mRNA
0	0	1	2	2	NM_140747.1	CG7460-RB (CG7460), mRNA
0	0	0	7	7	NM_141644.2	CG9379-RA (by), mRNA
0	0	0	5	9	NM_205882.1	CG4574-RF, transcript variant F (Plc21C), mRNA
0	0	0	5	9	NM_057503.4	CG4574-RB, transcript variant B (Plc21C), mRNA
0	0	0	5	9	NM_205883.1	CG4574-RD, transcript variant D (Plc21C), mRNA
0	0	0	5	9	NM_057504.4	CG4574-RA, transcript variant A (Plc21C), mRNA
0	0	0	5	9	NM_205884.1	CG4574-RC, transcript variant C (Plc21C), mRNA
0	0	0	5	5	NM_169429.3	CG14723-RA, transcript variant A (HisCl1), mRNA
0	0	0	5	5	NM_141859.3	CG14723-RB, transcript variant B (HisCl1), mRNA
0	0	0	5	3	NM_170281.1	CG5476-RA (CG5476), mRNA
0	0	0	5	3	NM_165574.1	CG30379-RA (CG30379), mRNA
0	0	0	4	6	NM_136256.2	CG8678-RA (CG8678), mRNA
0	0	0	4	3	NM_136068.2	CG17597-RA (CG17597), mRNA
0	0	0	4	3	NM_168416.1	CG32066-RB, transcript variant B (CG32066), mRNA
0	0	0	4	3	NM_168417.1	CG32066-RC, transcript variant C (CG32066), mRNA
0	0	0	4	2	NM_079726.3	CG5374-RA, transcript variant A (T-cp1), mRNA
0	0	0	4	2	NM_170016.1	CG5374-RB, transcript variant B (T-cp1), mRNA
0	0	0	3	8	NM_138059.1	CG13575-RA (CG13575), mRNA

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