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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4423R-4 |
| Symbol |
GstD6 |
Full Name |
Glutathione S transferase D6 |
| CG No |
CG4423 |
Old CG No |
CG4423 |
| Synonyms |
DmGSTD6, DmGST25, Gst, GST, gstD25, GstD25, CG4423, GstD6 |
| Accession No (Link to NCBI) |
NM_080176.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
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Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTATAACATGTCTGGTTCCCC |
| Primer Seq. 3' |
aatctagaggtaccACCATTTCGGTTGTGGAAACG |
| Predicted Fragment Size |
498 |
| IR fragment full Seq |
0001 CTATAACATG TCTGGTTCCC CGAGCACCCG GGCCGTTATG ATGACCGCCA AAGCTGTGGG
0061 AGTCGAGTTC AACTCGATAC AAGTTAATAC CTTTGTGGGG GAACAACTGG AGCCATGGTT
0121 TGTAAAGATA AACCCTCAGC ACACGATTCC AACCCTGGTG GACAATTTAT TCGTTATTTG
0181 GGAAACTCGG GCCATTGTTG TCTACCTAGT GGAGCAGTAC GGTAAGGATG ATTCTCTGTA
0241 CCCGAAGGAT CCCCAGAAGC AAGCGCTGAT CAACCAGCGT CTGTACTTCG ATATGGGCAC
0301 CCTGTACGAT GGCATTGCCA AGTACTTTTT CCCACTGCTT CGGACGGGCA AACCCGGAAC
0361 TCAGGAGAAT CTTGAGAAAC TGAACGCTGC CTTCGATCTT CTCAACAACT TCCTGGATGG
0421 CCAGGATTAC GTGGCCGGCA ATCAGCTTTC AGTGGCAGAT ATCGTTATAT TGGCCACCGT
0481 TTCCACAACC GAAATGGT |
| in silico PCR Fragment |
0001 CTATAACATG TCTGGTTCCC CGAGCACCCG GGCCGTTATG ATGACCGCCA AAGCTGTGGG
0061 AGTCGAGTTC AACTCGATAC AAGTTAATAC CTTTGTGGGG GAACAACTGG AGCCATGGTT
0121 TGTAAAGATA AACCCTCAGC ACACGATTCC AACCCTGGTG GACAATTTAT TCGTTATTTG
0181 GGAAACTCGG GCCATTGTTG TCTACCTAGT GGAGCAGTAC GGTAAGGATG ATTCTCTGTA
0241 CCCGAAGGAT CCCCAGAAGC AAGCGCTGAT CAACCAGCGT CTGTACTTCG ATATGGGCAC
0301 CCTGTACGAT GGCATTGCCA AGTACTTTTT CCCACTGCTT CGGACGGGCA AACCCGGAAC
0361 TCAGGAGAAT CTTGAGAAAC TGAACGCTGC CTTCGATCTT CTCAACAACT TCCTGGATGG
0421 CCAGGATTAC GTGGCCGGCA ATCAGCTTTC AGTGGCAGAT ATCGTTATAT TGGCCACCGT
0481 TTCCACAACC GAAATGGT |
| Assemble Data |
4423R-4.IR_full 1 CTATAACATGTCTGGTTCCCCGAGCACCCGGGCCGTTATGATGACCGCCAAAGCTGTGGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 1 CTATAACATGTCTGGTTCCCCGAGCACCCGGGCCGTTATGATGACCGCCAAAGCTGTGGG 60
4423R-4.IR_full 61 AGTCGAGTTCAACTCGATACAAGTTAATACCTTTGTGGGGGAACAACTGGAGCCATGGTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 61 AGTCGAGTTCAACTCGATACAAGTTAATACCTTTGTGGGGGAACAACTGGAGCCATGGTT 120
4423R-4.IR_full 121 TGTAAAGATAAACCCTCAGCACACGATTCCAACCCTGGTGGACAATTTATTCGTTATTTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 121 TGTAAAGATAAACCCTCAGCACACGATTCCAACCCTGGTGGACAATTTATTCGTTATTTG 180
4423R-4.IR_full 181 GGAAACTCGGGCCATTGTTGTCTACCTAGTGGAGCAGTACGGTAAGGATGATTCTCTGTA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 181 GGAAACTCGGGCCATTGTTGTCTACCTAGTGGAGCAGTACGGTAAGGATGATTCTCTGTA 240
4423R-4.IR_full 241 CCCGAAGGATCCCCAGAAGCAAGCGCTGATCAACCAGCGTCTGTACTTCGATATGGGCAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 241 CCCGAAGGATCCCCAGAAGCAAGCGCTGATCAACCAGCGTCTGTACTTCGATATGGGCAC 300
4423R-4.IR_full 301 CCTGTACGATGGCATTGCCAAGTACTTTTTCCCACTGCTTCGGACGGGCAAACCCGGAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 301 CCTGTACGATGGCATTGCCAAGTACTTTTTCCCACTGCTTCGGACGGGCAAACCCGGAAC 360
4423R-4.IR_full 361 TCAGGAGAATCTTGAGAAACTGAACGCTGCCTTCGATCTTCTCAACAACTTCCTGGATGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 361 TCAGGAGAATCTTGAGAAACTGAACGCTGCCTTCGATCTTCTCAACAACTTCCTGGATGG 420
4423R-4.IR_full 421 CCAGGATTACGTGGCCGGCAATCAGCTTTCAGTGGCAGATATCGTTATATTGGCCACCGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4423R-4.in silico 421 CCAGGATTACGTGGCCGGCAATCAGCTTTCAGTGGCAGATATCGTTATATTGGCCACCGT 480
4423R-4.IR_full 481 TTCCACAACCGAAATGGT 498
||||||||||||||||||
4423R-4.in silico 481 TTCCACAACCGAAATGGT 498
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
480 |
0 |
0 |
0 |
NM_080176.1 |
CG4423-RA (GstD6), mRNA |
| 1.66 |
8 |
5 |
13 |
72 |
NM_080375.1 |
CG4371-RA (GstD7), mRNA |
| 1.45 |
7 |
7 |
21 |
58 |
NM_080173.1 |
CG4181-RA (GstD2), mRNA |
| 0.2 |
1 |
3 |
4 |
5 |
NM_143006.1 |
CG13617-RA (CG13617), mRNA |
| 0 |
0 |
5 |
18 |
41 |
NM_080174.2 |
CG11512-RA (GstD4), mRNA |
| 0 |
0 |
4 |
10 |
22 |
NM_080177.1 |
CG4421-RA (GstD8), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_136404.2 |
CG3358-RB, transcript variant B (CG3358), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_165497.1 |
CG3358-RA, transcript variant A (CG3358), mRNA |
| 0 |
0 |
3 |
5 |
5 |
NM_137485.2 |
CG17531-RA (GstE7), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142619.1 |
CG4362-RA (CG4362), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141647.2 |
CG9386-RA (CG9386), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_166130.1 |
CG18255-RE, transcript variant E (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137538.1 |
CG15100-RA (CG15100), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170340.1 |
CG5643-RF, transcript variant F (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170339.1 |
CG5643-RE, transcript variant E (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143312.2 |
CG5643-RC, transcript variant C (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170336.1 |
CG5643-RA, transcript variant A (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170341.1 |
CG5643-RG, transcript variant G (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170338.1 |
CG5643-RD, transcript variant D (wdb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170337.1 |
CG5643-RB, transcript variant B (wdb), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043115.1 |
CG1066-RC, transcript variant C (Shab), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079068.2 |
CG11001-RA (FK506-bp2), mRNA |
| 0 |
0 |
0 |
19 |
57 |
NM_001038953.1 |
CG10045-RB, transcript variant B (GstD1), mRNA |
| 0 |
0 |
0 |
19 |
57 |
NM_079602.3 |
CG10045-RA, transcript variant A (GstD1), mRNA |
| 0 |
0 |
0 |
17 |
46 |
NM_144456.1 |
CG18548-RA (GstD10), mRNA |
| 0 |
0 |
0 |
14 |
59 |
NM_080175.2 |
CG12242-RA (GstD5), mRNA |
| 0 |
0 |
0 |
10 |
34 |
NM_141924.1 |
CG10091-RA (GstD9), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
 |
| |
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