NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4420R-3

Symbol CG4420 Full Name CG4420

CG No CG4420 Old CG No CG4420

Synonyms CG4420

Accession No (Link to NCBI) NM_132901.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGTGACGACCAGCGATGACA

Primer Seq. 3'

aatctagaggtaccGGAATCGATGGCTTCCGCCAG

Predicted Fragment Size

IR fragment full Seq

0001 CAGTGACGAC CaGCGATGAC AAGGTCTTCT GCTTGGACGT GGCCCAGGAT CTTGAGCTGG 
0061 AGAATCTCAA GGCCCTCTGC GCGATGGAGA TCGGTGCGGA AGTGTCCCAA ATAGCGGTAA 
0121 TTTTCAACGG GCGGGAGCTT TCCAGCGACA AACAGACGCT GCAACAGTGC GGAGTCGGCG 
0181 ACGGCGACTT CATCATGCTG GAACCGACGC AGGAGCGCCA ATCGCCCAGT TGGCGGAAAT 
0241 CCAGCGATCA GCACGCTCGA CTTCAGCAAC ATCGCCGTGC CGGGCACTAG TTCAGGCGGC 
0301 AGTCCACCGT CAAGGCGGGT GCAGCAGCAG GGAGTGCAGC AGCCGCAGTT CAACAACCTC 
0361 ACGGACATAC CCACGACTGA TGAGTTCAAT GTGAACTTCG ACGACGACCC AGAGACGGTG 
0421 CGACAGATGT TTCTGTCCAG TCCCGAGACT CTGTCGCTCC TCCGGCAGTA CAATCCCAGC 
0481 CTGGCGGAAG CCATCGANTT CC

in silico PCR Fragment

0001 CAGTGACGAC CAGCGATGAC AAGGTCTTCT GCTTGGACGT GGCCCAGGAT CTTGAGCTGG 
0061 AGAATCTCAA GGCCCTCTGC GCGATGGAGA TCGGTGCGGA AGTGTCCCAA ATAGCGGTAA 
0121 TTTTCAACGG GCGGGAGCTT TCCAGCGACA AACAGACGCT GCAACAGTGC GGAGTCGGCG 
0181 ACGGCGACTT CATCATGCTG GAA-CGACGC AGGAGCGCCA ATCGCCCAGT TGGCGGAAAT 
0241 CCAGCGATCA GCACGCTCGA CTTCAGCAAC ATCGCCGTGC CGGGCACTAG TTCAGGCGGC 
0301 AGTCCACCGT CAAGGCGGGT GCAGCAGCAG GGAGTGCAGC AGCCGCAGTT CAACAACCTC 
0361 ACGGACATAC CCACGACCGA TGAGTTCAAT GTGAACTTCG ACGACGACCC AGAGACGGTG 
0421 CGACAGATGT TTCTGTCCAG TCCCGAGACT CTGTCGCTCC TCCGGCAGTA CAATCCCAGC 
0481 CTGGCGGAAG CCATCGA-TT CC

Assemble Data


4420R-3.IR_full       1   CAGTGACGACCAGCGATGACAAGGTCTTCTGCTTGGACGTGGCCCAGGATCTTGAGCTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     1   CAGTGACGACCAGCGATGACAAGGTCTTCTGCTTGGACGTGGCCCAGGATCTTGAGCTGG 60


4420R-3.IR_full       61  AGAATCTCAAGGCCCTCTGCGCGATGGAGATCGGTGCGGAAGTGTCCCAAATAGCGGTAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     61  AGAATCTCAAGGCCCTCTGCGCGATGGAGATCGGTGCGGAAGTGTCCCAAATAGCGGTAA 120


4420R-3.IR_full       121 TTTTCAACGGGCGGGAGCTTTCCAGCGACAAACAGACGCTGCAACAGTGCGGAGTCGGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     121 TTTTCAACGGGCGGGAGCTTTCCAGCGACAAACAGACGCTGCAACAGTGCGGAGTCGGCG 180


4420R-3.IR_full       181 ACGGCGACTTCATCATGCTGGAACCGACGCAGGAGCGCCAATCGCCCAGTTGGCGGAAAT 240
                          ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
4420R-3.in silico     181 ACGGCGACTTCATCATGCTGGAA-CGACGCAGGAGCGCCAATCGCCCAGTTGGCGGAAAT 240


4420R-3.IR_full       241 CCAGCGATCAGCACGCTCGACTTCAGCAACATCGCCGTGCCGGGCACTAGTTCAGGCGGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     241 CCAGCGATCAGCACGCTCGACTTCAGCAACATCGCCGTGCCGGGCACTAGTTCAGGCGGC 300


4420R-3.IR_full       301 AGTCCACCGTCAAGGCGGGTGCAGCAGCAGGGAGTGCAGCAGCCGCAGTTCAACAACCTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     301 AGTCCACCGTCAAGGCGGGTGCAGCAGCAGGGAGTGCAGCAGCCGCAGTTCAACAACCTC 360


4420R-3.IR_full       361 ACGGACATACCCACGACTGATGAGTTCAATGTGAACTTCGACGACGACCCAGAGACGGTG 420
                          ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     361 ACGGACATACCCACGACCGATGAGTTCAATGTGAACTTCGACGACGACCCAGAGACGGTG 420


4420R-3.IR_full       421 CGACAGATGTTTCTGTCCAGTCCCGAGACTCTGTCGCTCCTCCGGCAGTACAATCCCAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4420R-3.in silico     421 CGACAGATGTTTCTGTCCAGTCCCGAGACTCTGTCGCTCCTCCGGCAGTACAATCCCAGC 480


4420R-3.IR_full       481 CTGGCGGAAGCCATCGANTTCC 502
                          ||||||||||||||||| ||||
4420R-3.in silico     481 CTGGCGGAAGCCATCGA-TTCC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_132901.2	CG4420-RA (CG4420), mRNA
0.41	2	2	2	8	NM_138041.2	CG4049-RA (CG4049), mRNA
0	0	4	3	6	NM_138088.2	CG11414-RA (CG11414), mRNA
0	0	2	7	34	NM_169147.2	CG31483-RA (Pif2), mRNA
0	0	2	4	4	NM_078581.2	CG1500-RA (fw), mRNA
0	0	2	3	8	NM_079282.1	CG3322-RA (LanB2), mRNA
0	0	1	8	8	NM_176037.1	CG32972-RB, transcript variant B (CG32972), mRNA
0	0	1	8	8	NM_205987.1	CG32972-RA, transcript variant A (CG32972), mRNA
0	0	1	4	3	NM_135480.2	CG4592-RA (CG4592), mRNA
0	0	1	4	2	NM_140573.4	CG32156-RE, transcript variant E (Mbs), mRNA
0	0	1	3	4	NM_140561.3	CG5931-RA (CG5931), mRNA
0	0	1	2	15	NM_132713.1	CG11584-RB (CG11584), mRNA
0	0	1	2	6	NM_137166.3	CG7761-RA (pcs), mRNA
0	0	1	2	6	NM_167852.1	CG9097-RB, transcript variant B (bab1), mRNA
0	0	0	5	14	NM_079700.1	CG3723-RA (Dhc93AB), mRNA
0	0	0	5	13	NM_132157.1	CG9650-RB, transcript variant B (CG9650), mRNA
0	0	0	5	13	NM_167121.1	CG9650-RC, transcript variant C (CG9650), mRNA
0	0	0	5	13	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	0	5	13	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	0	5	13	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	0	5	12	NM_167402.1	CG1517-RC, transcript variant C (na), mRNA
0	0	0	5	12	NM_167401.1	CG1517-RB, transcript variant B (na), mRNA
0	0	0	5	11	NM_167026.2	CG6824-RB, transcript variant B (ovo), mRNA
0	0	0	5	11	NM_167027.2	CG6824-RC, transcript variant C (ovo), mRNA
0	0	0	5	11	NM_001038742.1	CG6824-RD, transcript variant D (ovo), mRNA
0	0	0	5	11	NM_080338.3	CG6824-RA, transcript variant A (ovo), mRNA
0	0	0	5	7	NM_206441.1	CG31481-RB, transcript variant B (pb), mRNA
0	0	0	5	7	NM_057321.4	CG31481-RA, transcript variant A (pb), mRNA
0	0	0	5	7	NM_206439.1	CG31481-RD, transcript variant D (pb), mRNA
0	0	0	5	7	NM_206440.1	CG31481-RC, transcript variant C (pb), mRNA

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