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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4399R-3 
 Symbol east  Full Name enhanced adult sensory threshold 
 CG No CG4399  Old CG No CG4399 
 Synonyms l(1)G0500, EG:133E12.4, CG4399, l(1)G0014, anon-WO03040301.224, east 
 Accession No (Link to NCBI) NM_080125.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCAATGCCAGTGTGGAGATTC 
 Primer Seq. 3' 
aatctagaggtaccAGCTCGTGACTCTGATGCTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCAATGCCAG TGTGGAGATT CCAGCCAGCA GCGAGGAGCC AGCAATGGGC GTCGGCGAAG 
0061 AGATGTCTAT CATAAGCAAA ACACGCACCT CAACTTTGTC AGTGGAGCCC GCTAAGGAGC 
0121 CAACAGTAAC AGCAGAGCTG GAAGGCGAAA AAGAGCTGGA ATCGAATCCA GTCTCCAAAA 
0181 CTCCTAGGTC CACGCCTACG CCAACCCTTA CGCCAGCCGT CACGCCTACC GCCAGTGATG 
0241 GAGTGGCGGC CAAGAGCGTG AGGGTTACCC GGCACTCGTC GCCACTGCTT CTGATCATCT 
0301 CGCCCACGAC AAGTAGACGT GAGGTCGGCG ACGGAGAGTT AGACACCGAG GAACCAACGG 
0361 GATCGGGTGG CCAAAGAAAG AGCTCCGTGG AGCGATCTTT GGCGCCCGTT ATACGCGGAC 
0421 GAAAGTCCAT CAAGGATCTG AAAGAAGCCA AAGAAGTCAA GTCCGAGGAG CCGCCTGCCG 
0481 CAGCATCAGA GTCACGAGCT  
 in silico PCR Fragment 
0001 CCAATGCCAG TGTGGAGATT CCAGACAGCA GCGAGGAGCC AGCAATGGGC GTCGGCGAAG 
0061 AGATGTCTAT CATAAGCAAA ACACGCACCT CAACTTTGTC AGTGGAGCCC GCTAAGGAGC 
0121 CAACAGTAAC AGCAGAGCTG GAAGGCGAAA AAGAGCTGGA ATCGAATCCA GTCTCCAAAA 
0181 CTCCTAGGTC CACGCCTACG CCAACCCTTA CGCCAGCCGT CACGCCTACC GCCAGTGATG 
0241 GAGTGGCGGC CAAGAGCGTG AGGGTTACCC GGCACTCGTC GCCACTGCTT CTGATCATCT 
0301 CGCCCACGAC AAGTAGACGT GAGGTCGGCG ACGGAGAGCT AGACACCGAG GAACCAACGG 
0361 GATCGGGTGG CCAAAGAAAG AGCTCCGTGG AGCGATCTTT GGCGCCCGTT ATACGCGGAC 
0421 GAAAGTCCAT CAAGGATCTG AAAGAAGCCA AAGAAGTCAA GTCCGAGGAG CCGCCTGCCG 
0481 CAGCATCAGA GTCACGAGCT  
 Assemble Data 

4399R-3.IR_full       1   CCAATGCCAGTGTGGAGATTCCAGCCAGCAGCGAGGAGCCAGCAATGGGCGTCGGCGAAG 60
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
4399R-3.in silico     1   CCAATGCCAGTGTGGAGATTCCAGACAGCAGCGAGGAGCCAGCAATGGGCGTCGGCGAAG 60


4399R-3.IR_full       61  AGATGTCTATCATAAGCAAAACACGCACCTCAACTTTGTCAGTGGAGCCCGCTAAGGAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     61  AGATGTCTATCATAAGCAAAACACGCACCTCAACTTTGTCAGTGGAGCCCGCTAAGGAGC 120


4399R-3.IR_full       121 CAACAGTAACAGCAGAGCTGGAAGGCGAAAAAGAGCTGGAATCGAATCCAGTCTCCAAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     121 CAACAGTAACAGCAGAGCTGGAAGGCGAAAAAGAGCTGGAATCGAATCCAGTCTCCAAAA 180


4399R-3.IR_full       181 CTCCTAGGTCCACGCCTACGCCAACCCTTACGCCAGCCGTCACGCCTACCGCCAGTGATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     181 CTCCTAGGTCCACGCCTACGCCAACCCTTACGCCAGCCGTCACGCCTACCGCCAGTGATG 240


4399R-3.IR_full       241 GAGTGGCGGCCAAGAGCGTGAGGGTTACCCGGCACTCGTCGCCACTGCTTCTGATCATCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     241 GAGTGGCGGCCAAGAGCGTGAGGGTTACCCGGCACTCGTCGCCACTGCTTCTGATCATCT 300


4399R-3.IR_full       301 CGCCCACGACAAGTAGACGTGAGGTCGGCGACGGAGAGTTAGACACCGAGGAACCAACGG 360
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
4399R-3.in silico     301 CGCCCACGACAAGTAGACGTGAGGTCGGCGACGGAGAGCTAGACACCGAGGAACCAACGG 360


4399R-3.IR_full       361 GATCGGGTGGCCAAAGAAAGAGCTCCGTGGAGCGATCTTTGGCGCCCGTTATACGCGGAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     361 GATCGGGTGGCCAAAGAAAGAGCTCCGTGGAGCGATCTTTGGCGCCCGTTATACGCGGAC 420


4399R-3.IR_full       421 GAAAGTCCATCAAGGATCTGAAAGAAGCCAAAGAAGTCAAGTCCGAGGAGCCGCCTGCCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4399R-3.in silico     421 GAAAGTCCATCAAGGATCTGAAAGAAGCCAAAGAAGTCAAGTCCGAGGAGCCGCCTGCCG 480


4399R-3.IR_full       481 CAGCATCAGAGTCACGAGCT 500
                          ||||||||||||||||||||
4399R-3.in silico     481 CAGCATCAGAGTCACGAGCT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_080125.2  CG4399-RB (east), mRNA 
0   0  3  2  3  NM_139995.1  CG6511-RA (CG6511), mRNA 
0   0  2  4  3  NM_169311.1  CG8495-RC, transcript variant C (RpS29), mRNA 
0   0  2  3  2  NM_080097.2  CG6699-RA (beta'Cop), mRNA 
0   0  2  2  9  NM_137707.2  CG30388-RA (Magi), mRNA 
0   0  1  6  9  NM_137690.2  CG10543-RA, transcript variant A (CG10543), mRNA 
0   0  1  4  3  NM_079700.1  CG3723-RA (Dhc93AB), mRNA 
0   0  1  4  2  NM_170567.1  CG1856-RD, transcript variant D (ttk), mRNA 
0   0  1  4  2  NM_080172.2  CG1856-RC, transcript variant C (ttk), mRNA 
0   0  1  4  2  NM_170568.1  CG1856-RF, transcript variant F (ttk), mRNA 
0   0  1  3  8  NM_079449.2  CG7978-RA (Ac76E), mRNA 
0   0  1  3  5  NM_136675.1  CG1625-RA (CG1625), mRNA 
0   0  1  3  3  NM_136002.1  CG15142-RA (CG15142), mRNA 
0   0  1  3  3  NM_080152.2  CG5092-RA (Tor), mRNA 
0   0  1  2  4  NM_167188.1  CG32703-RA (CG32703), mRNA 
0   0  1  1  1  NM_001043263.1  CG34157-RF, transcript variant F (Dys), mRNA 
0   0  1  1  1  NM_079016.3  CG8523-RA (Mdr50), mRNA 
0   0  1  1  1  NM_001043261.1  CG34157-RG, transcript variant G (Dys), mRNA 
0   0  1  1  1  NM_001043258.1  CG34157-RA, transcript variant A (Dys), mRNA 
0   0  1  1  1  NM_001043259.1  CG34157-RC, transcript variant C (Dys), mRNA 
0   0  1  1  1  NM_001043257.1  CG34157-RH, transcript variant H (Dys), mRNA 
0   0  0  6  10  NM_001014694.1  CG1464-RC, transcript variant C (ey), mRNA 
0   0  0  6  10  NM_079889.2  CG1464-RA, transcript variant A (ey), mRNA 
0   0  0  6  10  NM_001014693.1  CG1464-RD, transcript variant D (ey), mRNA 
0   0  0  6  10  NM_166789.1  CG1464-RB, transcript variant B (ey), mRNA 
0   0  0  5  7  NM_078649.2  CG9842-RA (Pp2B-14D), mRNA 
0   0  0  5  6  NM_176544.1  CG33193-RA (sav), mRNA 
0   0  0  5  4  NM_135357.3  CG8183-RA, transcript variant A (Khc-73), mRNA 
0   0  0  5  4  NM_176176.1  CG8183-RB, transcript variant B (Khc-73), mRNA 
0   0  0  4  4  NM_165255.1  CG10283-RB, transcript variant B (CG10283), mRNA 

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