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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4363R-2 |
| Symbol |
CG4363 |
Full Name |
CG4363 |
| CG No |
CG4363 |
Old CG No |
CG4363 |
| Synonyms |
CT14230, BcDNA:LP06375, CG4363 |
| Accession No (Link to NCBI) |
NM_137769.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGGCCCAAACAATGGCGAAG |
| Primer Seq. 3' |
aatctagaggtaccCAGTTGGCATCGTTCAGACAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGGCCCAAA CAATGGCGAA GATTTTGAGT GCGGTGGTGC TGGTGGTTAT GGTGGGTCAT
0061 GTGGCCGCTA ACACGGCAAT CAGTTGCCAC ACTTGTGAAG GAGCTAATTG CCAAAGGGTT
0121 CAACTGAGTA AAACGCAGAC ATGTGTGGAT TCCCTGGNCT ATTGTGTGAC CATCTACGAT
0181 GAAGCCAAGG TTTTGTTCAA GGGTTGCTCC CTCGAGATTC CGAAGGACTT GCGTTCCAAA
0241 TGCAATGACA ATCGCTCTTG CCACAAGTGC AACACGAAAG AATGCAACAA TGTGGGATCT
0301 GCGAAGTACG CGTGCATTCA GTGCGATTCC AGCAAGGATT CCAATTGCGC TTCTAATGCC
0361 GCCGCTTTGG AAGCAGCCCG TTGCAGTGCC CCCACGGCTC CAAACTCGTA TTGCTATGTG
0421 AAATCCTCGG GAGGATCCAT TGTGCgTGGA TGCTCCACCA CCGAAACCGA TcAGCAGAGC
0481 TGTCTGAACG ATGCCAACTG |
| in silico PCR Fragment |
0001 AAGGCCCAAA CAATGGCGAA GATTTTGAGT GCTGTGGTGC TGGTGGTTAT GGTGGGTCAT
0061 GTGGCCGCTA ACACGGCAAT CAGTTGCCAC ACTTGTGAAG GAGCTAATTG CCAAAGGGTT
0121 CAACTGAGTA AAACGCAGAC ATGTGTGGAT TCCCTGGACT ATTGTGTGAC CATCTACGAT
0181 GAAGCCAAGG TTTTGTTCAA GGGTTGCTCC CTCGAGATTC CGAAGGACTT GCGTTCCAAA
0241 TGCAATGACA ATCGCTCTTG CCACAAGTGC AACACGAAAG AATGCAACAA TGTGGGATCT
0301 GCGAAGTACG CGTGCATTCA GTGCGATTCC AGCAAGGATT CCAATTGCGC TTCTAATGCC
0361 GCCGCTTTGG AAGCAGCCCG TTGCAGTGCC CCCACGGCTC CAAACTCGTA TTGCTATGTG
0421 AAATCCTCGG GAGGATCCAT TGTGCGTGGA TGCTCCACCA CCGAAACCGA TCAGCAGAGC
0481 TGTCTGAACG ATGCCAACTG |
| Assemble Data |
4363R-2.IR_full 1 AAGGCCCAAACAATGGCGAAGATTTTGAGTGCGGTGGTGCTGGTGGTTATGGTGGGTCAT 60
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
4363R-2.in silico 1 AAGGCCCAAACAATGGCGAAGATTTTGAGTGCTGTGGTGCTGGTGGTTATGGTGGGTCAT 60
4363R-2.IR_full 61 GTGGCCGCTAACACGGCAATCAGTTGCCACACTTGTGAAGGAGCTAATTGCCAAAGGGTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 61 GTGGCCGCTAACACGGCAATCAGTTGCCACACTTGTGAAGGAGCTAATTGCCAAAGGGTT 120
4363R-2.IR_full 121 CAACTGAGTAAAACGCAGACATGTGTGGATTCCCTGGNCTATTGTGTGACCATCTACGAT 180
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
4363R-2.in silico 121 CAACTGAGTAAAACGCAGACATGTGTGGATTCCCTGGACTATTGTGTGACCATCTACGAT 180
4363R-2.IR_full 181 GAAGCCAAGGTTTTGTTCAAGGGTTGCTCCCTCGAGATTCCGAAGGACTTGCGTTCCAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 181 GAAGCCAAGGTTTTGTTCAAGGGTTGCTCCCTCGAGATTCCGAAGGACTTGCGTTCCAAA 240
4363R-2.IR_full 241 TGCAATGACAATCGCTCTTGCCACAAGTGCAACACGAAAGAATGCAACAATGTGGGATCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 241 TGCAATGACAATCGCTCTTGCCACAAGTGCAACACGAAAGAATGCAACAATGTGGGATCT 300
4363R-2.IR_full 301 GCGAAGTACGCGTGCATTCAGTGCGATTCCAGCAAGGATTCCAATTGCGCTTCTAATGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 301 GCGAAGTACGCGTGCATTCAGTGCGATTCCAGCAAGGATTCCAATTGCGCTTCTAATGCC 360
4363R-2.IR_full 361 GCCGCTTTGGAAGCAGCCCGTTGCAGTGCCCCCACGGCTCCAAACTCGTATTGCTATGTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 361 GCCGCTTTGGAAGCAGCCCGTTGCAGTGCCCCCACGGCTCCAAACTCGTATTGCTATGTG 420
4363R-2.IR_full 421 AAATCCTCGGGAGGATCCATTGTGCGTGGATGCTCCACCACCGAAACCGATCAGCAGAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4363R-2.in silico 421 AAATCCTCGGGAGGATCCATTGTGCGTGGATGCTCCACCACCGAAACCGATCAGCAGAGC 480
4363R-2.IR_full 481 TGTCTGAACGATGCCAACTG 500
||||||||||||||||||||
4363R-2.in silico 481 TGTCTGAACGATGCCAACTG 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137769.2 |
CG4363-RA (CG4363), mRNA |
| 0 |
0 |
5 |
5 |
24 |
NM_133083.1 |
CG15040-RA (CG15040), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_139495.1 |
CG9973-RA (CG9973), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168178.1 |
CG10121-RD, transcript variant D (SP1173), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168177.2 |
CG10121-RC, transcript variant C (SP1173), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168176.1 |
CG10121-RA, transcript variant A (SP1173), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_139796.2 |
CG10121-RB, transcript variant B (SP1173), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137834.1 |
CG6018-RA (CG6018), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_079668.2 |
CG7467-RB, transcript variant B (osa), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169775.1 |
CG7467-RA, transcript variant A (osa), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_165044.1 |
CG31846-RA (CG31846), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_131989.2 |
CG4208-RA (XRCC1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_142995.2 |
CG5762-RA (CG5762), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137488.2 |
CG12263-RA (CG12263), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_168218.1 |
CG32372-RA (CG32372), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079677.2 |
CG6027-RA (cdi), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137290.3 |
CG4750-RA (CG4750), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043299.1 |
CG33553-RG, transcript variant G (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014680.1 |
CG33553-RA, transcript variant A (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043300.1 |
CG33553-RF, transcript variant F (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014682.1 |
CG33553-RB, transcript variant B (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014678.1 |
CG33553-RE, transcript variant E (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014681.1 |
CG33553-RD, transcript variant D (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014679.1 |
CG33553-RC, transcript variant C (Doa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142125.2 |
CG7825-RA (Rad17), mRNA |
| 0 |
0 |
0 |
7 |
22 |
NM_140417.2 |
CG17364-RA, transcript variant A (CG17364), mRNA |
| 0 |
0 |
0 |
7 |
20 |
NM_176328.1 |
CG17364-RB, transcript variant B (CG17364), mRNA |
| 0 |
0 |
0 |
7 |
13 |
NM_170361.1 |
CG5514-RA, transcript variant A (CG5514), mRNA |
| 0 |
0 |
0 |
7 |
13 |
NM_143342.1 |
CG5514-RB, transcript variant B (CG5514), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
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