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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4335R-4 
 Symbol CG4335  Full Name CG4335 
 CG No CG4335  Old CG No CG4335 
 Synonyms CG4335 
 Accession No (Link to NCBI) NM_142629.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCTTTTGAAGCACCCGAAAAC 
 Primer Seq. 3' 
aatctagaggtaccTCCACATTTCGCCAAAGAACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCTTTTGAAG CACCCGAAAA CCAGCCAATC AATAGAAATC AATGAGTTTT GGCTCCGTGA 
0061 TCATTGTCGA TGTGTGGAGT GTCTAAATTT CGAAACAAAT CAGAGGCGAT ACGATGTCTT 
0121 AGATCTACCA GCTGATATAA TGCCACTAGA TGTCAAATAC GACGGGATGA ATCTACAAGT 
0181 GCAGTGGAGT GATGCTCACA AGTCGAACTA TGACCTGGAC TTTATCTTCG ACTCACAGCT 
0241 GGAGAGGTTG ATAGGTCGGC GATCAAAGTC CACTAATCTG ACACCATGGA ATCGTAGCAT 
0301 TATCTTGCAA AACGAACGGC ACTTACGATT CCCATTGCCC CAGCTGGTTT CCAGTGATAA 
0361 CGAAGTCAGA TCCCTGGTTG AATCTCTGGT GCGTTATGGC ATTGTCTTTA TCGATGATGT 
0421 GGCTCCCACC GCGAATATGA CAGAATTgGN TCTGCGACGA GTTTTCCCCC TTATGAAAAC 
0481 GTTCTTTGGC GAAATGTGGA  
 in silico PCR Fragment 
0001 GCTTTTGAAG CACCCGAAAA CCAGCCAATC AATAGAAATC AATGAGTTTT GGCTCCGTGA 
0061 TCATTGTCGA TGTGTGGAGT GTCTAAATTT CGAAACAAAT CAGAGGCGAT ACGATGTCTT 
0121 AGATCTACCA GCTGATATAA TGCCACTAGA TGTCAAATAC GACGGGATGA ATCTACAAGT 
0181 GCAGTGGAGT GATGCTCACA AGTCGAACTA TGACCTGGAC TTTATCTTCG ACTCACAGCT 
0241 GGAGAGGTTG ATAGGTCGGC GATCAAAGTC CACTAATCTG ACACCATGGA ATCGTAGCAT 
0301 TATCTTGCAA AACGAACGGC ACTTACGATT CCCATTGCCC CAGCTGGTTT CCAGTGATAA 
0361 CGAAGTCAGA TCCCTGGTTG AATCTCTGGT GCGTTATGGC ATTGTCTTTA TCGATGATGT 
0421 GGCTCCCACC GCGAATATGA CAGAATTGGC TCTGCGACGA GTTTTCCCCC TTATGAAAAC 
0481 GTTCTTTGGC GAAATGTGGA  
 Assemble Data 

4335R-4.IR_full       1   GCTTTTGAAGCACCCGAAAACCAGCCAATCAATAGAAATCAATGAGTTTTGGCTCCGTGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     1   GCTTTTGAAGCACCCGAAAACCAGCCAATCAATAGAAATCAATGAGTTTTGGCTCCGTGA 60


4335R-4.IR_full       61  TCATTGTCGATGTGTGGAGTGTCTAAATTTCGAAACAAATCAGAGGCGATACGATGTCTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     61  TCATTGTCGATGTGTGGAGTGTCTAAATTTCGAAACAAATCAGAGGCGATACGATGTCTT 120


4335R-4.IR_full       121 AGATCTACCAGCTGATATAATGCCACTAGATGTCAAATACGACGGGATGAATCTACAAGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     121 AGATCTACCAGCTGATATAATGCCACTAGATGTCAAATACGACGGGATGAATCTACAAGT 180


4335R-4.IR_full       181 GCAGTGGAGTGATGCTCACAAGTCGAACTATGACCTGGACTTTATCTTCGACTCACAGCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     181 GCAGTGGAGTGATGCTCACAAGTCGAACTATGACCTGGACTTTATCTTCGACTCACAGCT 240


4335R-4.IR_full       241 GGAGAGGTTGATAGGTCGGCGATCAAAGTCCACTAATCTGACACCATGGAATCGTAGCAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     241 GGAGAGGTTGATAGGTCGGCGATCAAAGTCCACTAATCTGACACCATGGAATCGTAGCAT 300


4335R-4.IR_full       301 TATCTTGCAAAACGAACGGCACTTACGATTCCCATTGCCCCAGCTGGTTTCCAGTGATAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     301 TATCTTGCAAAACGAACGGCACTTACGATTCCCATTGCCCCAGCTGGTTTCCAGTGATAA 360


4335R-4.IR_full       361 CGAAGTCAGATCCCTGGTTGAATCTCTGGTGCGTTATGGCATTGTCTTTATCGATGATGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4335R-4.in silico     361 CGAAGTCAGATCCCTGGTTGAATCTCTGGTGCGTTATGGCATTGTCTTTATCGATGATGT 420


4335R-4.IR_full       421 GGCTCCCACCGCGAATATGACAGAATTGGNTCTGCGACGAGTTTTCCCCCTTATGAAAAC 480
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
4335R-4.in silico     421 GGCTCCCACCGCGAATATGACAGAATTGGCTCTGCGACGAGTTTTCCCCCTTATGAAAAC 480


4335R-4.IR_full       481 GTTCTTTGGCGAAATGTGGA 500
                          ||||||||||||||||||||
4335R-4.in silico     481 GTTCTTTGGCGAAATGTGGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_142629.2  CG4335-RA (CG4335), mRNA 
0   0  1  4  2  NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   0  1  2  2  NM_138236.1  CG9122-RA (Trh), mRNA 
0   0  1  2  2  NM_206292.1  CG7526-RB, transcript variant B (CG7526), mRNA 
0   0  1  2  2  NM_139880.2  CG7526-RA, transcript variant A (CG7526), mRNA 
0   0  1  2  2  NM_078855.2  CG7644-RA (beat-Ib), mRNA 
0   0  0  5  3  NM_134932.2  CG2772-RA (CG2772), mRNA 
0   0  0  5  3  NM_079167.3  CG12002-RA, transcript variant A (Pxn), mRNA 
0   0  0  5  3  NM_206255.1  CG12002-RC, transcript variant C (Pxn), mRNA 
0   0  0  5  3  NM_206254.1  CG12002-RD, transcript variant D (Pxn), mRNA 
0   0  0  5  3  NM_206253.1  CG12002-RE, transcript variant E (Pxn), mRNA 
0   0  0  4  5  NM_167875.1  CG9181-RD, transcript variant D (Ptp61F), mRNA 
0   0  0  4  5  NM_057339.3  CG9181-RA, transcript variant A (Ptp61F), mRNA 
0   0  0  4  3  NM_080186.2  CG2830-RA (Hsp60B), mRNA 
0   0  0  4  3  NM_144176.1  CG12521-RA (CG12521), mRNA 
0   0  0  4  3  NM_141666.2  CG9492-RA (CG9492), mRNA 
0   0  0  4  3  NM_176104.1  CG33087-RC (CG33087), mRNA 
0   0  0  4  2  NM_144367.1  CG6633-RA (Ugt86Dd), mRNA 
0   0  0  4  2  NM_137302.2  CG8098-RA, transcript variant A (Picot), mRNA 
0   0  0  4  2  NM_166187.1  CG8098-RB, transcript variant B (Picot), mRNA 
0   0  0  3  8  NM_079222.2  CG8339-RA (sfl), mRNA 
0   0  0  3  7  NM_057343.3  CG9327-RA (Pros29), mRNA 
0   0  0  3  5  NM_166137.1  CG8405-RA (CG8405), mRNA 
0   0  0  3  4  NM_078865.2  CG6794-RA, transcript variant A (Dif), mRNA 
0   0  0  3  4  NM_168912.1  CG7597-RB, transcript variant B (CG7597), mRNA 
0   0  0  3  4  NM_141068.2  CG7597-RA, transcript variant A (CG7597), mRNA 
0   0  0  3  3  NM_167843.1  CG13887-RC, transcript variant C (CG13887), mRNA 
0   0  0  3  3  NM_138218.2  CG13887-RB, transcript variant B (CG13887), mRNA 
0   0  0  3  2  NM_164378.1  CG3625-RA, transcript variant A (CG3625), mRNA 
0   0  0  3  2  NM_134670.2  CG3625-RB, transcript variant B (CG3625), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
+- 
DB
+- 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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