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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4323R-1 
 Symbol CG4323  Full Name CG4323 
 CG No CG4323  Old CG No CG4323 
 Synonyms CG4323 
 Accession No (Link to NCBI) NM_169896.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTCCTACAACCAGCGGCGT 
 Primer Seq. 3' 
aatctagaggtaccTCCACGGAGCGCGATCATCGA 
 Predicted Fragment Size 
498 
 IR fragment full Seq 
0001 ATGTCCTACA ACCAGCGGCG TATATGCCCG CTGGTACTTT GGAGGTCTGA GCTGGAGCTC 
0061 CATGAGCTNC CATNGTGACT CATANCGATT GACTTGATCA AGGTGCTTAT CCAAACTCAG 
0121 GCGGAGAAGC TATCGGTATT CCAGANCACC CGCAAGATTG TCAAGGAACA GGGACCTCTG 
0181 GCCATGTACA ATGGCATCTC GVCCTCAATG CTGCGCCAAT ACACGTACAC ATTGGCCCGT 
0241 TTTGGCATTT ATTCGGTGGG CTCTGGAGCT ATGGACACCA GCACCATGGC CGGCAAAACA 
0301 TGTCTAGCCG CGATTGCCGG TGGAATTGGC GGGTTTGTGG GCGCTCCTGC AGATCTGATC 
0361 AACGTCCGCC TGCAGAACGA TGTCAAGTTG CCACCAGAGA AGAGGCGCAA CTATAAGCAC 
0421 GCCATAGACG GACTGGTGCG AATCACCCGC GAAGAGGGAT GGAAAAATCT CTTCAATGGC 
0481 TCCTCGATGA TCGCGCTCCG TGGA 
 in silico PCR Fragment 
0001 ATGTCCTACA ACCAGCGGCG TAT-TGCCCG CTGGTACTTT GGAGGTCTG- GC--GAGCTC 
0061 CATG-GCTGC CATGGTGACT CAT-CCGATT GACTTGATCA AGGTGCTTAT CCAAACTCAG 
0121 GCGGAGAAGC TATCGGTATT CCAGACCACC CGCAAGATTG TCAAGGAACA GGGACCTCTG 
0181 GCCATGTACA ATGGCATCTC GGCCTCAATG CTGCGCCAAT ACACGTACAC ATTGGCCCGT 
0241 TTTGGCATTT ATTCGGTGGG CTCTGGAGCT ATGGACACCA GCACCATGGC CGGCAAAACA 
0301 TGTCTAGCCG CGATTGCCGG TGGAATTGGC GGGTTTGTGG GCGCTCCTGC AGATCTGATC 
0361 AACGTCCGCC TGCAGAACGA TGTCAAGTTG CCACCAGAGA AGAGGCGCAA CTATAAGCAC 
0421 GCCATAGACG GACTGGTGCG AATCACCCGC GAAGAGGGAT GGAAAAATCT CTTCAATGGC 
0481 TCCTCGATGA TCGCGCTCCG TGGA 
 Assemble Data 

4323R-1.IR_full       1   ATGTCCTACAACCAGCGGCGTATATGCCCGCTGGTACTTTGGAGGTCTGAGCTGGAGCTC 60
                          ||||||||||||||||||||||| ||||||||||||||||||||||||| ||  ||||||
4323R-1.in silico     1   ATGTCCTACAACCAGCGGCGTAT-TGCCCGCTGGTACTTTGGAGGTCTG-GC--GAGCTC 60


4323R-1.IR_full       61  CATGAGCTNCCATNGTGACTCATANCGATTGACTTGATCAAGGTGCTTATCCAAACTCAG 120
                          |||| ||| |||| |||||||||  |||||||||||||||||||||||||||||||||||
4323R-1.in silico     61  CATG-GCTGCCATGGTGACTCAT-CCGATTGACTTGATCAAGGTGCTTATCCAAACTCAG 120


4323R-1.IR_full       121 GCGGAGAAGCTATCGGTATTCCAGANCACCCGCAAGATTGTCAAGGAACAGGGACCTCTG 180
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
4323R-1.in silico     121 GCGGAGAAGCTATCGGTATTCCAGACCACCCGCAAGATTGTCAAGGAACAGGGACCTCTG 180


4323R-1.IR_full       181 GCCATGTACAATGGCATCTCGVCCTCAATGCTGCGCCAATACACGTACACATTGGCCCGT 240
                          ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
4323R-1.in silico     181 GCCATGTACAATGGCATCTCGGCCTCAATGCTGCGCCAATACACGTACACATTGGCCCGT 240


4323R-1.IR_full       241 TTTGGCATTTATTCGGTGGGCTCTGGAGCTATGGACACCAGCACCATGGCCGGCAAAACA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4323R-1.in silico     241 TTTGGCATTTATTCGGTGGGCTCTGGAGCTATGGACACCAGCACCATGGCCGGCAAAACA 300


4323R-1.IR_full       301 TGTCTAGCCGCGATTGCCGGTGGAATTGGCGGGTTTGTGGGCGCTCCTGCAGATCTGATC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4323R-1.in silico     301 TGTCTAGCCGCGATTGCCGGTGGAATTGGCGGGTTTGTGGGCGCTCCTGCAGATCTGATC 360


4323R-1.IR_full       361 AACGTCCGCCTGCAGAACGATGTCAAGTTGCCACCAGAGAAGAGGCGCAACTATAAGCAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4323R-1.in silico     361 AACGTCCGCCTGCAGAACGATGTCAAGTTGCCACCAGAGAAGAGGCGCAACTATAAGCAC 420


4323R-1.IR_full       421 GCCATAGACGGACTGGTGCGAATCACCCGCGAAGAGGGATGGAAAAATCTCTTCAATGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4323R-1.in silico     421 GCCATAGACGGACTGGTGCGAATCACCCGCGAAGAGGGATGGAAAAATCTCTTCAATGGC 480


4323R-1.IR_full       481 TCCTCGATGATCGCGCTCCGTGGA 504
                          ||||||||||||||||||||||||
4323R-1.in silico     481 TCCTCGATGATCGCGCTCCGTGGA 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   480  0  0  1  NM_169896.1  CG4323-RA, transcript variant A (CG4323), mRNA 
100   480  0  0  1  NM_169897.1  CG4323-RB, transcript variant B (CG4323), mRNA 
0   0  4  7  14  NM_136500.2  CG11196-RA (CG11196), mRNA 
0   0  3  2  5  NM_001015387.1  CG40042-PA.3 (CG40042), mRNA 
0   0  3  2  4  NM_001015386.1  CG40041-PB.3 (CG40041), mRNA 
0   0  2  4  2  NM_176177.1  CG33139-RA (Ranbp11), mRNA 
0   0  2  3  3  NM_141653.2  CG16789-RA (CG16789), mRNA 
0   0  2  2  5  NM_132288.1  CG15365-RA (CG15365), mRNA 
0   0  2  2  3  NM_080022.2  CG2841-RB, transcript variant B (ptr), mRNA 
0   0  2  2  3  NM_166937.1  CG2841-RA, transcript variant A (ptr), mRNA 
0   0  2  2  3  NM_166938.1  CG2841-RC, transcript variant C (ptr), mRNA 
0   0  2  2  3  NM_166075.1  CG30475-RA (CG30475), mRNA 
0   0  1  5  4  NM_137560.1  CG10474-RA (CG10474), mRNA 
0   0  1  5  3  NM_079699.2  CG5685-RA, transcript variant A (Calx), mRNA 
0   0  1  5  3  NM_169941.1  CG5685-RC, transcript variant C (Calx), mRNA 
0   0  1  5  3  NM_169940.1  CG5685-RB, transcript variant B (Calx), mRNA 
0   0  1  5  2  NM_139437.1  CG15879-RA (CG15879), mRNA 
0   0  1  4  2  NM_205880.1  CG2674-RE, transcript variant E (M(2)21AB), mRNA 
0   0  1  4  2  NM_164361.1  CG2674-RB, transcript variant B (M(2)21AB), mRNA 
0   0  1  4  2  NM_164356.1  CG2674-RA, transcript variant A (M(2)21AB), mRNA 
0   0  1  4  2  NM_164359.1  CG2674-RH, transcript variant H (M(2)21AB), mRNA 
0   0  1  4  2  NM_080184.1  CG2674-RC, transcript variant C (M(2)21AB), mRNA 
0   0  1  4  2  NM_164357.1  CG2674-RD, transcript variant D (M(2)21AB), mRNA 
0   0  1  4  2  NM_164360.1  CG2674-RI, transcript variant I (M(2)21AB), mRNA 
0   0  1  4  2  NM_164358.1  CG2674-RF, transcript variant F (M(2)21AB), mRNA 
0   0  1  4  2  NM_164355.1  CG2674-RJ, transcript variant J (M(2)21AB), mRNA 
0   0  1  4  2  NM_164362.1  CG2674-RG, transcript variant G (M(2)21AB), mRNA 
0   0  1  3  5  NM_080292.2  CG3972-RA (Cyp4g1), mRNA 
0   0  1  3  2  NM_170076.1  CG4370-RB, transcript variant B (Irk2), mRNA 
0   0  1  3  2  NM_142892.2  CG4370-RA, transcript variant A (Irk2), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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