NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4237R-1

Symbol Gap69C Full Name GTPase-activating protein 69C

CG No CG4237 Old CG No CG4237

Synonyms i51, CG4237, GAP69C, ARF1-GAP, Gap69C

Accession No (Link to NCBI)

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCTCCGTCACTATGGATAAG

Primer Seq. 3'

aatctagaggtaccGTTTTCGGTGGTGGTTCGCGT

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


4237R-1.IR full       1   CGCTCCGTCACTATNGATAAGTGGAAGGACATCGAGCTGGAGAAGATGAAGGCGGGCGGC 60
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
4237R-1.in silico     1   CGCTCCGTCACTATGGATAAGTGGAAGGACATCGAGCTGGAGAAGATGAAGGCGGGCGGC 60


4237R-1.IR full       61  AATCNNAATGCGCGCGAGTTTCTGGAGGATCAGGAGGACTGGAACGAACGGGCGCCCATC 120
                          ||||  |||||||||||||||||||||||||||||||||||||||||||||||||| |||
4237R-1.in silico     61  AATCGCAATGCGCGCGAGTTTCTGGAGGATCAGGAGGACTGGAACGAACGGGCGCCTATC 120


4237R-1.IR full       121 ACGCAGCGTTACAACTCGAAGGCGGCTGCTTTGTACCGCGATAAGATAGCCACCTTGGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4237R-1.in silico     121 ACGCAGCGTTACAACTCGAAGGCGGCTGCTTTGTACCGCGATAAGATAGCCACCTTGGCC 180


4237R-1.IR full       181 CAGGGCAAGAGCTGGGATCTCAAGGAGGCGCAAGGTCGCGTGGGCAGCAACAATAGCTTC 240
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4237R-1.in silico     181 CAGGGCAAGAGCTGGGATCTCAAGGAGGCGCAAGGCCGCGTGGGCAGCAACAATAGCTTC 240


4237R-1.IR full       241 AGCTCAGGTGGCTCCAGCAACAGCAGCTACCAGTCGCGACCCAGTGCAACAGGTTATGGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4237R-1.in silico     241 AGCTCAGGTGGCTCCAGCAACAGCAGCTACCAGTCGCGACCCAGTGCAACAGGTTATGGC 300


4237R-1.IR full       301 GGAAACGGAGGCTATCAGAATGGCGGAGGCGCCGAACCTGGAGGCTATCAGCAGTACCAA 360
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4237R-1.in silico     301 GGAAACGGAGGCTATCAGAATGGCGGAGGCGCCGAGCCTGGAGGCTATCAGCAGTACCAA 360


4237R-1.IR full       361 ACGCAGGAGTTCAAGGACCAGAAGGAGGAGTTCTTCAGCCGACGCCAAGTGGAGAATGCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4237R-1.in silico     361 ACGCAGGAGTTCAAGGACCAGAAGGAGGAGTTCTTCAGCCGACGCCAAGTGGAGAATGCC 420


4237R-1.IR full       421 TCCAGGCCAGAAAACTTGCCACCGAGTCAGGGCGGAAAATATGCAGGTTTCGGGTTCACA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4237R-1.in silico     421 TCCAGGCCAGAAAACTTGCCACCGAGTCAGGGCGGAAAATATGCAGGTTTCGGGTTCACA 480


4237R-1.IR full       481 CGCGAACCACCACCGAAAAC 500
                          ||||||||||||||||||||
4237R-1.in silico     481 CGCGAACCACCACCGAAAAC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc