|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4192R-3 |
| Symbol |
kek3 |
Full Name |
kekkon-3 |
| CG No |
CG4192 |
Old CG No |
CG4192 |
| Synonyms |
CT12629, BG:DS04862.1, CG4192, kek3 |
| Accession No (Link to NCBI) |
NM_078851.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N.
An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity.
Proc Natl Acad Sci U S A (2020) 117(1) 464-471
[
PubMed ID = 31852821
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTCAGGACATAGCCAGCGACA |
| Primer Seq. 3' |
aatctagaggtaccAGGGTATTGCCGAGAGCAGGT |
| Predicted Fragment Size |
477 |
| IR fragment full Seq |
0001 GTCAGGACAT AGCCAGCGAC AACAGCGCGC CAGcGCCGCA CGCTGGCGAC GAAGGTGCGT
0061 CGAAAAGGGC CACGCCCCCA ACGGCGCCTG CACCCGCCCC TGCGCCCTCG NCTGCCGCTC
0121 CATTTGCACC TGCTACTCTG GCTGCTGTGC TGCTGTTCTC AGCTGGGCCA GCTGAGGGCC
0181 GAGTGTCCAG CGGTGTGCGA GTGCAAGTGG AAGAGTGGCA AGGAGTCCGT CTTGTGCCTT
0241 AACGCCAACC TAACCCACAT CCCGCAGCCG CTGGACGCGG GAACTCAGTT GCTGGACCTT
0301 AGCGGCAATG AGATCCAACT AATACCCGAC GATAGCTTCG CAACCGCCCA GTTGCTCAAC
0361 CTACAGAAGG TGTACCTGGC CAGGTGTCAC CTTCGGCTTA TCGAACGCCA TGCCTTCCGT
0421 AAGCTGATCA ATCTAGTGGA ACTGGATCTA AGCCAGAACC TGCTCTCGGC AATACCCT |
| in silico PCR Fragment |
0001 GTCAGGACAT AGCCAGCGAC AACAGCGC-C CAGCGCCGCA CGCTGGCGAC GAAGGTGCGT
0061 CGAAAAGGGC CACGCCCCCA ACGGCGCCTG CACCCGCCCC TGCGCCCTCG CCTGCCGCTC
0121 CATTTGCACC TGCTACTCTG GCTGCTGTGC TGCTGTTCTC AGCTGGGCCA GCTGAGGGCC
0181 GAGTGTCCAG CGGTGTGCGA GTGCAAGTGG AAGAGTGGCA AGGAGTCCGT CTTGTGCCTT
0241 AACGCCAACC TAACCCACAT CCCGCAGCCG CTGGACGCGG GAACTCAGTT GCTGGACCTT
0301 AGCGGCAATG AGATCCAACT AATACCCGAC GATAGCTTCG CAACCGCCCA GTTGCTCAAC
0361 CTACAGAAGG TGTACCTGGC CAGGTGTCAC CTCCGGCTTA TCGAACGCCA TGCCTTCCGT
0421 AAGCTGATCA ATCTAGTGGA ACTGGATCTA AGCCAGAACC TGCTCTCGGC AATACCCT |
| Assemble Data |
4192R-3.IR_full 1 GTCAGGACATAGCCAGCGACAACAGCGCGCCAGCGCCGCACGCTGGCGACGAAGGTGCGT 60
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
4192R-3.in silico 1 GTCAGGACATAGCCAGCGACAACAGCGC-CCAGCGCCGCACGCTGGCGACGAAGGTGCGT 60
4192R-3.IR_full 61 CGAAAAGGGCCACGCCCCCAACGGCGCCTGCACCCGCCCCTGCGCCCTCGNCTGCCGCTC 120
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
4192R-3.in silico 61 CGAAAAGGGCCACGCCCCCAACGGCGCCTGCACCCGCCCCTGCGCCCTCGCCTGCCGCTC 120
4192R-3.IR_full 121 CATTTGCACCTGCTACTCTGGCTGCTGTGCTGCTGTTCTCAGCTGGGCCAGCTGAGGGCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-3.in silico 121 CATTTGCACCTGCTACTCTGGCTGCTGTGCTGCTGTTCTCAGCTGGGCCAGCTGAGGGCC 180
4192R-3.IR_full 181 GAGTGTCCAGCGGTGTGCGAGTGCAAGTGGAAGAGTGGCAAGGAGTCCGTCTTGTGCCTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-3.in silico 181 GAGTGTCCAGCGGTGTGCGAGTGCAAGTGGAAGAGTGGCAAGGAGTCCGTCTTGTGCCTT 240
4192R-3.IR_full 241 AACGCCAACCTAACCCACATCCCGCAGCCGCTGGACGCGGGAACTCAGTTGCTGGACCTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-3.in silico 241 AACGCCAACCTAACCCACATCCCGCAGCCGCTGGACGCGGGAACTCAGTTGCTGGACCTT 300
4192R-3.IR_full 301 AGCGGCAATGAGATCCAACTAATACCCGACGATAGCTTCGCAACCGCCCAGTTGCTCAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-3.in silico 301 AGCGGCAATGAGATCCAACTAATACCCGACGATAGCTTCGCAACCGCCCAGTTGCTCAAC 360
4192R-3.IR_full 361 CTACAGAAGGTGTACCTGGCCAGGTGTCACCTTCGGCTTATCGAACGCCATGCCTTCCGT 420
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
4192R-3.in silico 361 CTACAGAAGGTGTACCTGGCCAGGTGTCACCTCCGGCTTATCGAACGCCATGCCTTCCGT 420
4192R-3.IR_full 421 AAGCTGATCAATCTAGTGGAACTGGATCTAAGCCAGAACCTGCTCTCGGCAATACCCT 478
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-3.in silico 421 AAGCTGATCAATCTAGTGGAACTGGATCTAAGCCAGAACCTGCTCTCGGCAATACCCT 478
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
459 |
1 |
2 |
4 |
NM_078851.2 |
CG4192-RA (kek3), mRNA |
| 0 |
0 |
4 |
4 |
3 |
NM_167101.1 |
CG3039-RB, transcript variant B (ogre), mRNA |
| 0 |
0 |
4 |
4 |
3 |
NM_080085.2 |
CG3039-RA, transcript variant A (ogre), mRNA |
| 0 |
0 |
3 |
4 |
15 |
NM_078835.2 |
CG12283-RA (kek1), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_134498.2 |
CG14216-RA (CG14216), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_138037.1 |
CG10339-RA (CG10339), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137394.1 |
CG11423-RA (CG11423), mRNA |
| 0 |
0 |
1 |
8 |
16 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169450.1 |
CG14741-RA (CG14741), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135675.2 |
CG14939-RA (CG14939), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165555.1 |
CG30497-RB, transcript variant B (CG30497), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135910.1 |
CG15256-RA (CG15256), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132804.1 |
CG9203-RA (CG9203), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_079668.2 |
CG7467-RB, transcript variant B (osa), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_169775.1 |
CG7467-RA, transcript variant A (osa), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_206506.1 |
CG7467-RC, transcript variant C (osa), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169504.1 |
CG32473-RC, transcript variant C (CG32473), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142019.2 |
CG32473-RB, transcript variant B (CG32473), mRNA |
| 0 |
0 |
0 |
6 |
14 |
NM_167627.1 |
CG6659-RB, transcript variant B (CG6659), mRNA |
| 0 |
0 |
0 |
6 |
14 |
NM_133089.2 |
CG6659-RA, transcript variant A (CG6659), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_169816.1 |
CG14307-RB, transcript variant B (fru), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_079673.2 |
CG14307-RF, transcript variant F (fru), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_078963.2 |
CG7576-RA (Rab3), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_079747.2 |
CG5394-RA, transcript variant A (Aats-glupro), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_170103.1 |
CG5394-RB, transcript variant B (Aats-glupro), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136137.2 |
CG10137-RA (CG10137), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_132103.2 |
CG3973-RA (CG3973), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_206516.1 |
CG14307-RI, transcript variant I (fru), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_206515.1 |
CG14307-RJ, transcript variant J (fru), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_169819.1 |
CG14307-RE, transcript variant E (fru), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
|
|
|
|
