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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4164R-4 |
| Symbol |
CG4164 |
Full Name |
CG4164 |
| CG No |
CG4164 |
Old CG No |
CG4164 |
| Synonyms |
CG4164 |
| Accession No (Link to NCBI) |
NM_134681.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTGAAGAAGGCGTATCGCCGT |
| Primer Seq. 3' |
aatctagaggtaccAATGTGCGCTCCTCGTTGACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTGAAGAAGG CGTATCGCCG TTTGGCCAAG GAGCTGCATC CGGATAAGAA CAAGGACGAC
0061 CCGGATGCCT CTCACAAAGT TCCAGGACCT GGGAGCGGCC TACGAAGTCC TCTCCAATCC
0121 GGACAAACGG AAGACCTACG ACCGCTGCGG CGAGGNAATG CCTCAAGAAG GAGGGCATGA
0181 TGGATCACGG TGGTGATCCG TTCTCTAGCT TCTTTGGGGA CTTCGGCTTT CACTTCGGTG
0241 GTGATGGCCA GCAGCAANGN TGCTNCgCGA GGCGCCGATA TCGTAATGGA CTTGTACGTT
0301 TCCCTGGAGG AGCTATACTC CGGAAACTTT GTGGAAATTG TGAGGAACAA GCCTGTAACG
0361 AAACCCgNCT CAGGCACCAG GAAATGCAAC TGCCGCCAGG AGATGGTCAC CCGGAACCTT
0421 GGACCCGGGC GCTTCCAGAT GATCCAACAG ACGGTGTGCG ACGAGTGTCC CAACGTGAAG
0481 CTAGTCAACG AGGAGCGCAC ATT |
| in silico PCR Fragment |
0001 GTGAAGAAGG CGTATCGCCG TTTGGCCAAG GAGCTGCATC CGGATAAGAA CAAGGACGAC
0061 CCGGATGCCT C-CACAAAGT TCCAGGACCT GGGAGCGGCC TACGAAGTCC TCTCCAATCC
0121 GGACAAACGG AAGACCTACG ACCGCTGCGG CGAGG-AATG CCTCAAGAAG GAGGGCATGA
0181 TGGATCACGG TGGTGATCCG TTCTCTAGCT TCTTTGGGGA CTTCGGCTTT CACTTCGGTG
0241 GTGATGGCCA GCAGCAA-GA TGCTCCGCGA GGCGCCGATA TCGTAATGGA CTTGTACGTT
0301 TCCCTGGAGG AGCTATACTC CGGAAACTTT GTGGAAATTG TGAGGAACAA GCCTGTAACG
0361 AAACCCGCCT CAGGCACCAG GAAATGCAAC TGCCGCCAGG AGATGGTCAC CCGGAACCTT
0421 GGACCCGGGC GCTTCCAGAT GATCCAACAG ACGGTGTGCG ACGAGTGTCC CAACGTGAAG
0481 CTAGTCAACG AGGAGCGCAC ATT |
| Assemble Data |
4164R-4.IR_full 1 TGAAGAAGGCGTATCGCCGTTTGGCCAAGGAGCTGCATCCGGATAAGAACAAGGACGAC 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 1 TGAAGAAGGCGTATCGCCGTTTGGCCAAGGAGCTGCATCCGGATAAGAACAAGGACGAC 59
4164R-4.IR_full 61 CCGGATGCCTCTCACAAAGTTCCAGGACCTGGGAGCGGCCTACGAAGTCCTCTCCAATCC 119
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 61 CCGGATGCCTC-CACAAAGTTCCAGGACCTGGGAGCGGCCTACGAAGTCCTCTCCAATCC 119
4164R-4.IR_full 121 GGACAAACGGAAGACCTACGACCGCTGCGGCGAGGNAATGCCTCAAGAAGGAGGGCATGA 179
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4164R-4.in silico 121 GGACAAACGGAAGACCTACGACCGCTGCGGCGAGG-AATGCCTCAAGAAGGAGGGCATGA 179
4164R-4.IR_full 181 TGGATCACGGTGGTGATCCGTTCTCTAGCTTCTTTGGGGACTTCGGCTTTCACTTCGGTG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 181 TGGATCACGGTGGTGATCCGTTCTCTAGCTTCTTTGGGGACTTCGGCTTTCACTTCGGTG 239
4164R-4.IR_full 241 GTGATGGCCAGCAGCAANGNTGCTNCGCGAGGCGCCGATATCGTAATGGACTTGTACGTT 299
||||||||||||||||| | |||| |||||||||||||||||||||||||||||||||||
4164R-4.in silico 241 GTGATGGCCAGCAGCAA-GATGCTCCGCGAGGCGCCGATATCGTAATGGACTTGTACGTT 299
4164R-4.IR_full 301 TCCCTGGAGGAGCTATACTCCGGAAACTTTGTGGAAATTGTGAGGAACAAGCCTGTAACG 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 301 TCCCTGGAGGAGCTATACTCCGGAAACTTTGTGGAAATTGTGAGGAACAAGCCTGTAACG 359
4164R-4.IR_full 361 AAACCCGNCTCAGGCACCAGGAAATGCAACTGCCGCCAGGAGATGGTCACCCGGAACCTT 419
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 361 AAACCCGCCTCAGGCACCAGGAAATGCAACTGCCGCCAGGAGATGGTCACCCGGAACCTT 419
4164R-4.IR_full 421 GGACCCGGGCGCTTCCAGATGATCCAACAGACGGTGTGCGACGAGTGTCCCAACGTGAAG 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4164R-4.in silico 421 GGACCCGGGCGCTTCCAGATGATCCAACAGACGGTGTGCGACGAGTGTCCCAACGTGAAG 479
4164R-4.IR_full 481 CTAGTCAACGAGGAGCGCACATT 502
|||||||||||||||||||||||
4164R-4.in silico 481 CTAGTCAACGAGGAGCGCACATT 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_134681.2 |
CG4164-RA (CG4164), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_140053.2 |
CG3967-RC, transcript variant C (CG3967), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170630.1 |
CG3967-RD, transcript variant D (CG3967), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170631.1 |
CG3967-RE, transcript variant E (CG3967), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_140052.1 |
CG3967-RA, transcript variant A (CG3967), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167884.1 |
CG1009-RE, transcript variant E (Psa), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167883.1 |
CG1009-RC, transcript variant C (Psa), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167887.1 |
CG1009-RF, transcript variant F (Psa), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167885.1 |
CG1009-RA, transcript variant A (Psa), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_139360.2 |
CG1009-RB, transcript variant B (Psa), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167886.1 |
CG1009-RD, transcript variant D (Psa), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_080215.2 |
CG18525-RA, transcript variant A (Spn5), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_176501.1 |
CG18525-RB, transcript variant B (Spn5), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_135267.1 |
CG5155-RA (CG5155), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080041.2 |
CG7935-RA (msk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134669.2 |
CG11601-RA (CG11601), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078905.2 |
CG12847-RA (Tsp42Ec), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167344.1 |
CG32644-RB (CG32644), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080333.2 |
CG3346-RA (pon), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142474.1 |
CG14304-RA (CG14304), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_136993.2 |
CG3955-RA (CG3955), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_137991.1 |
CG4763-RA (CG4763), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_165490.1 |
CG3427-RA (Epac), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_206209.1 |
CG5504-RC, transcript variant C (l(2)tid), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_080193.3 |
CG5504-RA, transcript variant A (l(2)tid), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_206210.1 |
CG5504-RB, transcript variant B (l(2)tid), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_138142.3 |
CG2790-RA (CG2790), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176212.1 |
CG33197-RA, transcript variant A (mbl), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_168413.1 |
CG32062-RB, transcript variant B (CG32062), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_168412.1 |
CG32062-RD, transcript variant D (CG32062), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| ++ |
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| VM |
 |
| + |
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| VB |
 |
| |
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| Dcompartment |
|
Penetrance |
| + |
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