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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4153R-2 |
| Symbol |
eIF-2beta |
Full Name |
Eukaryotic initiation factor 2beta |
| CG No |
CG4153 |
Old CG No |
CG4153 |
| Synonyms |
CG4153, eIF2beta, D-eIF2beta, IF2B, elF2beta, eIF-2, eIF-2beta, Eif2beta |
| Accession No (Link to NCBI) |
NM_079319.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Sekine Y, Takagahara S, Hatanaka R, Watanabe T, Oguchi H, Noguchi T, Naguro I, Kobayashi K, Tsunoda M, Funatsu T, Nomura H, Toyoda T, Matsuki N, Kuranaga E, Miura M, Takeda K, Ichijo H.
p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors.
J Cell Sci (2011) 124(Pt 17) 3006-16
[
PubMed ID = 21878507
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATCTGGAGAGTTTCGGCAAG |
| Primer Seq. 3' |
aatctagaggtaccAACTCGGCCAGCAGGAAATCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NATCTGGAGA GTTTCGGCAA GAAGAAGAAA AAGAAGAAGA AGCCCTTCAA CATGGACGAA
0061 ATCGAAGCGG CCATACCCAG CTTTGGCGGC GATGATGTGG CCGCCAGCGA GGAGCCCGAG
0121 GAGGAGGAGA TCAATCTGGA CATGGACTTC TCGATGGCCA AAAAGAAGAA GAAGAGCAAG
0181 AAGAAGGAAC TGGACGAGCT GTTTGCGGAC CAAGCGGACG ACGACAAGAG CGAAGACAAA
0241 GAGAACGACG AGGACAACAG CTCAACCTGG TTTGGCTCCG ACCGCGATTA CACATACGAC
0301 GAGTTGCTGA AGCGGGTCTT CGAAATCATT CTCGACAAGA ATCCGGANCA TGGCCgccgG
0361 CCGAAAGCCA AAGTTCGTGA TNCGACCGCC GCAgGNNCTG CGCGTGGGAA CCAAGAAGAC
0421 CTCCTTTGCC AACTTCATNG NCATTgNGAA AACGCtGCAT CGCCTGCCCA AGCATCTGCT
0481 CGATTTCCTG CTGGCCGAGT T |
| in silico PCR Fragment |
0001 GATCTGGAGA GTTTCGGCAA GAAGAAGAAA AAGAAGAAGA AGCCCTTCAA CATGGACGAA
0061 ATCGAAGCGG CCATACCCAG CTTTGGCGGC GATGATGTGG CCGCCAGCGA GGAGCCCGAG
0121 GAGGAGGAGA TCAATCTGGA CATGGACTTC TCGATGGCCA AAAAGAAGAA GAAGAGCAAG
0181 AAGAAGGAAC TGGACGAGCT GTTTGCGGAC CAAGCGGACG ACGACAAGAG CGAAGACAAA
0241 GAGAACGACG AGGACAACAG CTCAACCTGG TTTGGCTCCG ACCGCGATTA CACATACGAC
0301 GAGTTGCTGA AGCGGGTCTT CGAAATCATT CTCGACAAGA ATCCGGA-CA TGGCCGCCGG
0361 CCGAAAGCCA AAGTTCGTGA TGCGACCGCC GCAGGTGCTG CGCGTGGGAA CCAAGAAGAC
0421 CTCCTTTGCC AACTTCATGG ACATTGCGAA AACGCTGCAT CGCCTGCCCA AGCATCTGCT
0481 CGATTTCCTG CTGGCCGAGT T |
| Assemble Data |
4153R-2.IR_full 1 ATCTGGAGAGTTTCGGCAAGAAGAAGAAAAAGAAGAAGAAGCCCTTCAACATGGACGAA 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4153R-2.in silico 1 ATCTGGAGAGTTTCGGCAAGAAGAAGAAAAAGAAGAAGAAGCCCTTCAACATGGACGAA 59
4153R-2.IR_full 61 ATCGAAGCGGCCATACCCAGCTTTGGCGGCGATGATGTGGCCGCCAGCGAGGAGCCCGAG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4153R-2.in silico 61 ATCGAAGCGGCCATACCCAGCTTTGGCGGCGATGATGTGGCCGCCAGCGAGGAGCCCGAG 119
4153R-2.IR_full 121 GAGGAGGAGATCAATCTGGACATGGACTTCTCGATGGCCAAAAAGAAGAAGAAGAGCAAG 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4153R-2.in silico 121 GAGGAGGAGATCAATCTGGACATGGACTTCTCGATGGCCAAAAAGAAGAAGAAGAGCAAG 179
4153R-2.IR_full 181 AAGAAGGAACTGGACGAGCTGTTTGCGGACCAAGCGGACGACGACAAGAGCGAAGACAAA 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4153R-2.in silico 181 AAGAAGGAACTGGACGAGCTGTTTGCGGACCAAGCGGACGACGACAAGAGCGAAGACAAA 239
4153R-2.IR_full 241 GAGAACGACGAGGACAACAGCTCAACCTGGTTTGGCTCCGACCGCGATTACACATACGAC 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4153R-2.in silico 241 GAGAACGACGAGGACAACAGCTCAACCTGGTTTGGCTCCGACCGCGATTACACATACGAC 299
4153R-2.IR_full 301 GAGTTGCTGAAGCGGGTCTTCGAAATCATTCTCGACAAGAATCCGGANCATGGCCGCCGG 359
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
4153R-2.in silico 301 GAGTTGCTGAAGCGGGTCTTCGAAATCATTCTCGACAAGAATCCGGA-CATGGCCGCCGG 359
4153R-2.IR_full 361 CCGAAAGCCAAAGTTCGTGATNCGACCGCCGCAGGNNCTGCGCGTGGGAACCAAGAAGAC 419
||||||||||||||||||||| ||||||||||||| |||||||||||||||||||||||
4153R-2.in silico 361 CCGAAAGCCAAAGTTCGTGATGCGACCGCCGCAGGTGCTGCGCGTGGGAACCAAGAAGAC 419
4153R-2.IR_full 421 CTCCTTTGCCAACTTCATNGNCATTGNGAAAACGCTGCATCGCCTGCCCAAGCATCTGCT 479
|||||||||||||||||| | ||||| |||||||||||||||||||||||||||||||||
4153R-2.in silico 421 CTCCTTTGCCAACTTCATGGACATTGCGAAAACGCTGCATCGCCTGCCCAAGCATCTGCT 479
4153R-2.IR_full 481 CGATTTCCTGCTGGCCGAGTT 500
|||||||||||||||||||||
4153R-2.in silico 481 CGATTTCCTGCTGGCCGAGTT 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
2 |
33 |
76 |
NM_079319.2 |
CG4153-RA (eIF-2beta), mRNA |
| 1.45 |
7 |
2 |
19 |
50 |
NM_058064.3 |
CG10206-RA (nop5), mRNA |
| 1.24 |
6 |
2 |
5 |
22 |
NM_206391.1 |
CG33256-RA (CG33256), mRNA |
| 0.62 |
3 |
2 |
3 |
2 |
NM_167281.1 |
CG1725-RF, transcript variant F (dlg1), mRNA |
| 0.41 |
2 |
15 |
24 |
99 |
NM_001014547.1 |
CG3333-RB, transcript variant B (Nop60B), mRNA |
| 0.41 |
2 |
15 |
24 |
99 |
NM_080381.2 |
CG3333-RA, transcript variant A (Nop60B), mRNA |
| 0.41 |
2 |
8 |
19 |
62 |
NM_142783.2 |
CG13849-RA (Nop56), mRNA |
| 0.41 |
2 |
5 |
10 |
43 |
NM_166093.1 |
CG8174-RA, transcript variant A (SRPK), mRNA |
| 0.41 |
2 |
5 |
10 |
43 |
NM_137190.2 |
CG8174-RB, transcript variant B (SRPK), mRNA |
| 0.41 |
2 |
5 |
10 |
43 |
NM_166094.1 |
CG8174-RC, transcript variant C (SRPK), mRNA |
| 0.41 |
2 |
2 |
2 |
5 |
NM_057843.2 |
CG14029-RA, transcript variant A (vri), mRNA |
| 0.41 |
2 |
2 |
2 |
5 |
NM_164639.1 |
CG14029-RC, transcript variant C (vri), mRNA |
| 0.2 |
1 |
3 |
13 |
26 |
NM_175978.1 |
CG14026-RD, transcript variant D (tkv), mRNA |
| 0.2 |
1 |
3 |
8 |
31 |
NM_142742.2 |
CG6332-RA (CG6332), mRNA |
| 0.2 |
1 |
3 |
3 |
9 |
NM_001014548.1 |
CG11406-RB, transcript variant B (CG11406), mRNA |
| 0.2 |
1 |
2 |
7 |
11 |
NM_136456.2 |
CG1360-RA (CG1360), mRNA |
| 0.2 |
1 |
2 |
4 |
12 |
NM_143783.2 |
CG5824-RA (l(3)07882), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_135345.2 |
CG8475-RA, transcript variant A (CG8475), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_164797.1 |
CG8475-RB, transcript variant B (CG8475), mRNA |
| 0.2 |
1 |
2 |
2 |
8 |
NM_166393.1 |
CG30143-RA (CG30143), mRNA |
| 0 |
0 |
13 |
40 |
90 |
NM_131944.1 |
CG3546-RA (CG3546), mRNA |
| 0 |
0 |
11 |
24 |
34 |
NM_139541.1 |
CG12017-RA (CG12017), mRNA |
| 0 |
0 |
9 |
16 |
41 |
NM_134861.1 |
CG2843-RA (CG2843), mRNA |
| 0 |
0 |
9 |
14 |
53 |
NM_143015.2 |
CG13625-RA (CG13625), mRNA |
| 0 |
0 |
7 |
16 |
24 |
NM_140412.1 |
CG8833-RA (CG8833), mRNA |
| 0 |
0 |
7 |
5 |
37 |
NM_132790.1 |
CG9123-RA (CG9123), mRNA |
| 0 |
0 |
6 |
7 |
15 |
NM_140897.2 |
CG8103-RA, transcript variant A (Mi-2), mRNA |
| 0 |
0 |
6 |
7 |
15 |
NM_001014591.1 |
CG8103-RB, transcript variant B (Mi-2), mRNA |
| 0 |
0 |
5 |
10 |
33 |
NM_141353.1 |
CG15580-RA, transcript variant A (CG15580), mRNA |
| 0 |
0 |
5 |
10 |
33 |
NM_169124.1 |
CG15580-RB, transcript variant B (CG15580), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
|
