NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4151R-1

Symbol CG4151 Full Name CG4151

CG No CG4151 Old CG No CG4151

Synonyms CG4151

Accession No (Link to NCBI) NM_131999.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees male pupal lethal, female semi-lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATCGTGGCCATCCAGGCTGA

Primer Seq. 3'

aatctagaggtaccCATTGCCCTGGCAGCTCCACT

Predicted Fragment Size

IR fragment full Seq

0001 CATCGTGGCC ATCCAGGCTG ATGGCGATCG GCGTCCGTGT GTGGGTCGCT GCACCGGTCT 
0061 GTCCAGCGGC CAGAGTGTGT GCATCCGCAA CAAGGTGACC AATGTGTGCA CCCGCCTGCC 
0121 GGCCTGTCGT CTGCGGGAGA AGAATTGCCG CCGTCGGGAC AATGGACTCG AACCCATTCG 
0181 GNAGACATGC ATCACCCGCT GTCGCAACAT TCCCGGCACC AGCGGCGTGG GCCAGTGCGC 
0241 CAATCAGGCT GCGTCCTCGT CCCCAGTCCG ATGGCAAGCG CATCAAGGAG TGCCAGAGGA 
0301 GGATCTGCCT TGACGACAAG CTGGCCAGCT GCTGGAGGGA CCAGCAGGGC GCTTTGCATC 
0361 CTGCAGACCC GCTGCGAGGC CCAGAGGAGG AACTGCGTCC GGAATCCGCT TTAACCAGTG 
0421 GGTNGAGGGC CAGCCAGTGG AGCTGCCAGG GCAATG

in silico PCR Fragment

0001 CATCGTGGCC ATCCAGGCTG ATGGCGATCG GCGTCCGTGT GTGGGTCGCT GCACCGGTCT 
0061 GTCCAGCGGC CAGAGTGTGT GCATCCGCAA CAAGGTGACC AATGTGTGCA CCCGCCTGCC 
0121 GGCCTGTCGT CTGCGGGAGA AGAATTGCCG CCGTCGGGAC AATGGACTCG AACCCATTCG 
0181 GGAGACATGC ATCACCCGCT GTCGCAACAT TCCCGGCACC AGCGGCGTGG GCCAGTGCGC 
0241 C-ATCAGGCT GCGTCCTCGT CCCCAGTCCG ATGGCAAGCG CATCAAGGAG TGCCAGAGGA 
0301 GGATCTGCCT TGACGACAAG CTGGCCAGCT GCTGGAGGGA CCAGCAGGGC GC-TTGCATC 
0361 CTGCAGACCC GCTGCGAGGC CCAGAGGAGG AACTGCGTCC GGAATCCGC- TTAACCAGTG 
0421 GGT-GAGGGC CAGCCAGTGG AGCTGCCAGG GCAATG

Assemble Data


4151R-1.IR_full       1   CATCGTGGCCATCCAGGCTGATGGCGATCGGCGTCCGTGTGTGGGTCGCTGCACCGGTCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4151R-1.in silico     1   CATCGTGGCCATCCAGGCTGATGGCGATCGGCGTCCGTGTGTGGGTCGCTGCACCGGTCT 60


4151R-1.IR_full       61  GTCCAGCGGCCAGAGTGTGTGCATCCGCAACAAGGTGACCAATGTGTGCACCCGCCTGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4151R-1.in silico     61  GTCCAGCGGCCAGAGTGTGTGCATCCGCAACAAGGTGACCAATGTGTGCACCCGCCTGCC 120


4151R-1.IR_full       121 GGCCTGTCGTCTGCGGGAGAAGAATTGCCGCCGTCGGGACAATGGACTCGAACCCATTCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4151R-1.in silico     121 GGCCTGTCGTCTGCGGGAGAAGAATTGCCGCCGTCGGGACAATGGACTCGAACCCATTCG 180


4151R-1.IR_full       181 GNAGACATGCATCACCCGCTGTCGCAACATTCCCGGCACCAGCGGCGTGGGCCAGTGCGC 240
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4151R-1.in silico     181 GGAGACATGCATCACCCGCTGTCGCAACATTCCCGGCACCAGCGGCGTGGGCCAGTGCGC 240


4151R-1.IR_full       241 CAATCAGGCTGCGTCCTCGTCCCCAGTCCGATGGCAAGCGCATCAAGGAGTGCCAGAGGA 300
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4151R-1.in silico     241 C-ATCAGGCTGCGTCCTCGTCCCCAGTCCGATGGCAAGCGCATCAAGGAGTGCCAGAGGA 300


4151R-1.IR_full       301 GGATCTGCCTTGACGACAAGCTGGCCAGCTGCTGGAGGGACCAGCAGGGCGCTTTGCATC 360
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
4151R-1.in silico     301 GGATCTGCCTTGACGACAAGCTGGCCAGCTGCTGGAGGGACCAGCAGGGCGC-TTGCATC 360


4151R-1.IR_full       361 CTGCAGACCCGCTGCGAGGCCCAGAGGAGGAACTGCGTCCGGAATCCGCTTTAACCAGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
4151R-1.in silico     361 CTGCAGACCCGCTGCGAGGCCCAGAGGAGGAACTGCGTCCGGAATCCGC-TTAACCAGTG 420


4151R-1.IR_full       421 GGTNGAGGGCCAGCCAGTGGAGCTGCCAGGGCAATG 456
                          ||| ||||||||||||||||||||||||||||||||
4151R-1.in silico     421 GGT-GAGGGCCAGCCAGTGGAGCTGCCAGGGCAATG 456

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	434	0	0	10	NM_131999.1	CG4151-RA (CG4151), mRNA
0	0	3	2	2	NM_137416.2	CG12298-RA (sub), mRNA
0	0	2	4	5	NM_132127.2	CG4542-RA (CG4542), mRNA
0	0	2	3	3	NM_140690.2	CG7729-RA (Fit2), mRNA
0	0	2	2	6	NM_164431.1	CG17654-RB, transcript variant B (Eno), mRNA
0	0	2	2	6	NM_164434.1	CG17654-RE, transcript variant E (Eno), mRNA
0	0	2	2	6	NM_164432.1	CG17654-RC, transcript variant C (Eno), mRNA
0	0	2	2	6	NM_164433.1	CG17654-RD, transcript variant D (Eno), mRNA
0	0	2	2	6	NM_058073.3	CG17654-RA, transcript variant A (Eno), mRNA
0	0	1	4	4	NM_142027.2	CG9799-RA (CG9799), mRNA
0	0	1	4	3	NM_168217.2	CG7546-RB, transcript variant B (CG7546), mRNA
0	0	1	4	3	NM_139875.3	CG7546-RA, transcript variant A (CG7546), mRNA
0	0	1	4	2	NM_136440.4	CG11139-RA (p47), mRNA
0	0	1	2	4	NM_169810.1	CG18617-RA, transcript variant A (Vha100-2), mRNA
0	0	1	2	4	NM_142465.1	CG18617-RB, transcript variant B (Vha100-2), mRNA
0	0	1	2	3	NM_140935.2	CG17145-RA (CG17145), mRNA
0	0	1	2	2	NM_135661.2	CG6509-RB, transcript variant B (CG6509), mRNA
0	0	1	2	2	NM_164967.1	CG6509-RA, transcript variant A (CG6509), mRNA
0	0	0	8	10	NM_139981.2	CG6831-RA (rhea), mRNA
0	0	0	6	9	NM_078523.2	CG2252-RB, transcript variant B (fs(1)h), mRNA
0	0	0	6	4	NM_169845.1	CG31043-RA, transcript variant A (gukh), mRNA
0	0	0	6	3	NM_166873.1	CG3638-RB, transcript variant B (CG3638), mRNA
0	0	0	6	3	NM_166872.1	CG3638-RD, transcript variant D (CG3638), mRNA
0	0	0	6	3	NM_130541.3	CG3638-RC, transcript variant C (CG3638), mRNA
0	0	0	6	3	NM_166874.1	CG3638-RA, transcript variant A (CG3638), mRNA
0	0	0	6	3	NM_169846.1	CG31043-RB, transcript variant B (gukh), mRNA
0	0	0	6	3	NM_001032021.1	CG31043-RC, transcript variant C (gukh), mRNA
0	0	0	5	3	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	0	4	5	NM_206116.1	CG7639-RB, transcript variant B (CG7639), mRNA
0	0	0	4	5	NM_137167.2	CG7639-RA, transcript variant A (CG7639), mRNA

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