NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4141R-1

Symbol

Pi3K92E

Full Name

Pi3K92E

CG No

CG4141

Old CG No

CG4141

Synonyms

Dp110, PI3K, p110, dp110, dPI3K, type-1 PI3K, Dp110/PI3K, PI-3-K, Pi3Kp110, PI(3)K, CG4141, PI3K-92E/Dp110, p120, PI3K-92D, Dmp110, rea, anon-92Ed, Pi3K92D, Pi3K92E, PI3K92E, PI3K-Dp110, dP110

Accession No (Link to NCBI)

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACCAGCCCAAGTATGAGACAC

Primer Seq. 3'

aatctagaggtaccCTCCAGTTCGATGTTGTCGCA

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


4141R-1.IR full       1   ACCAGCCCAAGTATGAGACACCGCCGGAAGAAGCGGAGCCGCCCTGCATGCGCTTTTCGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     1   ACCAGCCCAAGTATGAGACACCGCCGGAAGAAGCGGAGCCGCCCTGCATGCGCTTTTCGG 60


4141R-1.IR full       61  TTAACCTGTGGAAAAACGAGATGCTGAACTGGGTGGACCTAATCTGCCTGTTGCCCAATG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     61  TTAACCTGTGGAAAAACGAGATGCTGAACTGGGTGGACCTAATCTGCCTGTTGCCCAATG 120


4141R-1.IR full       121 GATTCCTGCTGGAGCTCAGGGTCAATCCGGCCAACACCATCCAGGTAATCAAGGTGGAGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     121 GATTCCTGCTGGAGCTCAGGGTCAATCCGGCCAACACCATCCAGGTAATCAAGGTGGAGA 180


4141R-1.IR full       181 TGGTCAACCAGGCCAAACAGATGCCACTGGGCTATGTGATCAAAGAGGCCTGCGAGTACC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     181 TGGTCAACCAGGCCAAACAGATGCCACTGGGCTATGTGATCAAAGAGGCCTGCGAGTACC 240


4141R-1.IR full       241 AGGTGTACGGCATCTCGACCTTCAACATCGAACCTTACACCGACGAAACGAAGCGACTCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     241 AGGTGTACGGCATCTCGACCTTCAACATCGAACCTTACACCGACGAAACGAAGCGACTCA 300


4141R-1.IR full       301 GTGAGGTCCAGCCGTACTTCGGCATCCTCAGCCTCGGCGAGCGCACCGACACAACGAGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     301 GTGAGGTCCAGCCGTACTTCGGCATCCTCAGCCTCGGCGAGCGCACCGACACAACGAGCT 360


4141R-1.IR full       361 TTAGCAGCGACTACGAGCTGACCAAGATGGTTAACGGAATGATCGGCACCACCTTCGATC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     361 TTAGCAGCGACTACGAGCTGACCAAGATGGTTAACGGAATGATCGGCACCACCTTCGATC 420


4141R-1.IR full       421 ATAATCGAACGCACGGCTCGCCCGAGATTGACGACTTCCGGCTGTATATGACCCAAACTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4141R-1.in silico     421 ATAATCGAACGCACGGCTCGCCCGAGATTGACGACTTCCGGCTGTATATGACCCAAACTT 480


4141R-1.IR full       481 GCGACAACATCGAACTGGAG 500
                          ||||||||||||||||||||
4141R-1.in silico     481 GCGACAACATCGAACTGGAG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc

GFP