NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4140R-3

Symbol CG4140 Full Name CG4140

CG No CG4140 Old CG No CG4140

Synonyms BG:DS00929.2, CG4140

Accession No (Link to NCBI) NM_135891.3

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees early pupal lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGGGGACTACGATTCCGAT

Primer Seq. 3'

aatctagaggtaccACCGTGGCCGTATTCCGACAA

Predicted Fragment Size

IR fragment full Seq

0001 ATGGGGGACT ACGATTCCGA TGATGAAAAA TCACAGGTGG AGAAGCTGCG CCACGCCAAA 
0061 GTGCCGCGCG GAATGTTCGT GAGCGGCTGG GACGACGATG CCGACGAACT GATCGAAGAG 
0121 GACAAGAACC CACAAAGCAG CATTGAACGC ATGATTCTGT GGGCGGTGAA CGAGAACCGC 
0181 ATCAGCGAGG TGCGGGAAAT CCTAAAACTG GACGCAGACA CCGTGAACGC CAAGGATAAC 
0241 GACGGATACA CGCCGCTGCA CCGAGCTGCC TATAATAACT TTGTGGATAT GGCCAAGTTG 
0301 CTGCTGCAGT ACCATGCCAA TCCCAACGCC CGCACGGAGC TGGGATGGAC GCCATTGCAC 
0361 TCCGCCTGCA AGTGGAACAA TGCGGATTGC GCCCATTTGC TGCTGCAGTT TGGCGCCGAT 
0421 GTAAATGCGG AATCGGACGG CAAGCAGACG CCGCTTCACA TAACCGCCAC TGTGTCCAAT 
0481 TGTCGGAANT ACGGCCACGG T

in silico PCR Fragment

0001 ATGGGGGACT ACGATTCCGA TGATGAAAAA TCACAGGTGG AGAAGCTGCG CCACGCCAAA 
0061 GTGCCGCGCG GAATGTTCGT GAGCGGCTGG GACGACGATG CCGACGAACT GATCGAAGAG 
0121 GACAAGAACC CACAAAGCAG CATTGAACGC ATGATTCTGT GGGCGGTGAA CGAGAACCGC 
0181 ATCAGCGAGG TGCGGGAAAT CCTAAAACTG GACGCAGACA CCGTGAACGC CAAGGATAAC 
0241 GACGGATACA CGCCGCTGCA CCGAGCTGCC TATAATAACT TTGTGGATAT GGCCAAGTTG 
0301 CTGCTGCAGT ACCATGCCAA TCCCAACGCC CGCACGGAGC TGGGATGGAC GCCATTGCAC 
0361 TCCGCCTGCA AGTGGAACAA TGCGGATTGC GCCCATTTGC TGCTGCAGTT TGGCGCCGAT 
0421 GTAAATGCGG AATCGGACGG CAAGCAGACG CCGCTTCACA TAACCGCCAC TGTGTCCAAT 
0481 TGTCGGAA-T ACGGCCACGG T

Assemble Data


4140R-3.IR_full       1   ATGGGGGACTACGATTCCGATGATGAAAAATCACAGGTGGAGAAGCTGCGCCACGCCAAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     1   ATGGGGGACTACGATTCCGATGATGAAAAATCACAGGTGGAGAAGCTGCGCCACGCCAAA 60


4140R-3.IR_full       61  GTGCCGCGCGGAATGTTCGTGAGCGGCTGGGACGACGATGCCGACGAACTGATCGAAGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     61  GTGCCGCGCGGAATGTTCGTGAGCGGCTGGGACGACGATGCCGACGAACTGATCGAAGAG 120


4140R-3.IR_full       121 GACAAGAACCCACAAAGCAGCATTGAACGCATGATTCTGTGGGCGGTGAACGAGAACCGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     121 GACAAGAACCCACAAAGCAGCATTGAACGCATGATTCTGTGGGCGGTGAACGAGAACCGC 180


4140R-3.IR_full       181 ATCAGCGAGGTGCGGGAAATCCTAAAACTGGACGCAGACACCGTGAACGCCAAGGATAAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     181 ATCAGCGAGGTGCGGGAAATCCTAAAACTGGACGCAGACACCGTGAACGCCAAGGATAAC 240


4140R-3.IR_full       241 GACGGATACACGCCGCTGCACCGAGCTGCCTATAATAACTTTGTGGATATGGCCAAGTTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     241 GACGGATACACGCCGCTGCACCGAGCTGCCTATAATAACTTTGTGGATATGGCCAAGTTG 300


4140R-3.IR_full       301 CTGCTGCAGTACCATGCCAATCCCAACGCCCGCACGGAGCTGGGATGGACGCCATTGCAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     301 CTGCTGCAGTACCATGCCAATCCCAACGCCCGCACGGAGCTGGGATGGACGCCATTGCAC 360


4140R-3.IR_full       361 TCCGCCTGCAAGTGGAACAATGCGGATTGCGCCCATTTGCTGCTGCAGTTTGGCGCCGAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     361 TCCGCCTGCAAGTGGAACAATGCGGATTGCGCCCATTTGCTGCTGCAGTTTGGCGCCGAT 420


4140R-3.IR_full       421 GTAAATGCGGAATCGGACGGCAAGCAGACGCCGCTTCACATAACCGCCACTGTGTCCAAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4140R-3.in silico     421 GTAAATGCGGAATCGGACGGCAAGCAGACGCCGCTTCACATAACCGCCACTGTGTCCAAT 480


4140R-3.IR_full       481 TGTCGGAANTACGGCCACGGT 501
                          |||||||| ||||||||||||
4140R-3.in silico     481 TGTCGGAA-TACGGCCACGGT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	6	NM_135891.3	CG4140-RA (CG4140), mRNA
0.2	1	5	4	9	NM_135994.1	CG5043-RA (CG5043), mRNA
0	0	4	4	8	NM_141542.1	CG11718-RA (CG11718), mRNA
0	0	4	2	3	NM_140464.1	CG17173-RA (CG17173), mRNA
0	0	3	2	2	NM_133164.2	CG10142-RA (Ance-5), mRNA
0	0	2	4	7	NM_168708.1	CG32167-RA (CG32167), mRNA
0	0	2	4	5	NM_140310.1	CG4328-RA (CG4328), mRNA
0	0	2	4	4	NM_136675.1	CG1625-RA (CG1625), mRNA
0	0	2	4	2	NR_002695.1	CR9337, mRNA
0	0	2	3	2	NM_135525.1	CG5604-RA (CG5604), mRNA
0	0	2	2	8	NM_057466.2	CG8896-RA (18w), mRNA
0	0	2	2	2	NM_135485.4	CG4619-RA (CG4619), mRNA
0	0	2	2	2	NM_135087.1	CG7371-RA (CG7371), mRNA
0	0	1	6	9	NM_169392.1	CG14704-RC, transcript variant C (PGRP-LB), mRNA
0	0	1	6	3	NM_134846.2	CG3227-RA (insv), mRNA
0	0	1	6	2	NM_079572.2	CG9484-RA (hyd), mRNA
0	0	1	4	8	NM_134490.2	CG12238-RA (l(1)G0084), mRNA
0	0	1	4	5	NM_170330.1	CG5954-RB, transcript variant B (l(3)mbt), mRNA
0	0	1	4	5	NM_079805.2	CG5954-RA, transcript variant A (l(3)mbt), mRNA
0	0	1	3	10	NM_143153.2	CG4719-RA (tankyrase), mRNA
0	0	1	3	5	NM_132780.1	CG15029-RA (CG15029), mRNA
0	0	1	3	4	NM_131919.1	CG15376-RA (CG15376), mRNA
0	0	1	3	4	NM_137331.2	CG9000-RA (CG9000), mRNA
0	0	1	3	3	NM_141289.2	CG2519-RA, transcript variant A (CG2519), mRNA
0	0	1	3	3	NM_206431.1	CG2519-RB, transcript variant B (CG2519), mRNA
0	0	1	2	16	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	1	2	14	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	1	2	6	NM_078601.2	CG9533-RA (rut), mRNA
0	0	1	2	3	NM_079618.2	CG8599-RA (Su(var)3-7), mRNA
0	0	0	8	18	NM_079979.2	CG18497-RB, transcript variant B (spen), mRNA

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