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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4124R-2 
 Symbol PNUTS  Full Name PNUTS 
 CG No CG33526  Old CG No CG4124 
 Synonyms CG4124, CG31657, PNUTSDm, CG33526, PNUTS 
 Accession No (Link to NCBI) NM_001014454.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGTTAAATGATGCCATCAGGG 
 Primer Seq. 3' 
aatctagaggtaccTTCCAGATGTTCCACCTCGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGTTAAATGA TGCCATCAGG GCCATGAACT GGcCGCTGGT ACAGGAGATC CTGGAACTGC 
0061 TGCTCCTTTC TCCCGTGGAT GTCAATCGCC TCAAGATAAA CTCAGCACCT ATTCTGGTCA 
0121 AGGGGCTGTG CAAGGATGGC GGCAACGAAG GTGTACGCAT TCTAGCCAAG CGTTTGGTAG 
0181 AGCAGTGGCT GAAGATTGTG ACGGAAAACA CAAGCATGAC GATCCAAGGT GCTGCGCCAC 
0241 ATATTGCAGC CACTACTGCC TCTCAGGTTC CTGCATCCGT TTCTGGAGCT ggNTNCGCTT 
0301 CAGTTCCGGA CTCTGCCTCT TCTTCAGACA GCACTGATAG CGCCGGAGCA CCTGTGACGT 
0361 ATACCATCAC CTCGGCGGCC AACAGTAGCA ATTCCAACAG CATTACCATC AAGTGGAAGC 
0421 CTTTGTTGGA CAACCCAGAT GGTGGCGACG AAACGGATTC TGGCACACAA TCGAAAGACA 
0481 ACGAGGTGGA ACATCTGGAA  
 in silico PCR Fragment 
0001 GGTTAAATGA TGCCATCAGG GCCATGAACT GGCCGCTGGT ACAGGAGATC CTGGAACTGC 
0061 TGCTCCTTTC TCCCGTGGAT GTCAATCGCC TCAAGATAAA CTCAGCACCT ATTCTGGTCA 
0121 AGGGGCTGTG CAAGGATGGC GGCAACGAAG GTGTACGCAT TCTAGCCAAG CGTTTGGTAG 
0181 AGCAGTGGCT GAAGATTGTG ACGGAAAACA CAAGCATGAC GATCCAAGGT GCTGCGCCAC 
0241 ATATTGCAGC CACTACTGCC TCTCAGGTTC CTGCATCCGT TTCTGGAGCT GGCTCCGCTT 
0301 CAGTTCCGGA CTCTGCCTCT TCTTCAGACA GCACTGATAG CGCCGGAGCA CCTGTGACGT 
0361 ATACCATCAC CTCGGCGGCC AACAGTAGCA ATTCCAACAG CATTACCATC AAGTGGAAGC 
0421 CTTTGTTGGA CAACCCAGAT GGTGGCGACG AAACGGATTC TGGCACACAA TCGAAAGACA 
0481 ACGAGGTGGA ACATCTGGAA  
 Assemble Data 

4124R-2.IR_full       1   GGTTAAATGATGCCATCAGGGCCATGAACTGGCCGCTGGTACAGGAGATCCTGGAACTGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     1   GGTTAAATGATGCCATCAGGGCCATGAACTGGCCGCTGGTACAGGAGATCCTGGAACTGC 60


4124R-2.IR_full       61  TGCTCCTTTCTCCCGTGGATGTCAATCGCCTCAAGATAAACTCAGCACCTATTCTGGTCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     61  TGCTCCTTTCTCCCGTGGATGTCAATCGCCTCAAGATAAACTCAGCACCTATTCTGGTCA 120


4124R-2.IR_full       121 AGGGGCTGTGCAAGGATGGCGGCAACGAAGGTGTACGCATTCTAGCCAAGCGTTTGGTAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     121 AGGGGCTGTGCAAGGATGGCGGCAACGAAGGTGTACGCATTCTAGCCAAGCGTTTGGTAG 180


4124R-2.IR_full       181 AGCAGTGGCTGAAGATTGTGACGGAAAACACAAGCATGACGATCCAAGGTGCTGCGCCAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     181 AGCAGTGGCTGAAGATTGTGACGGAAAACACAAGCATGACGATCCAAGGTGCTGCGCCAC 240


4124R-2.IR_full       241 ATATTGCAGCCACTACTGCCTCTCAGGTTCCTGCATCCGTTTCTGGAGCTGGNTNCGCTT 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| | |||||
4124R-2.in silico     241 ATATTGCAGCCACTACTGCCTCTCAGGTTCCTGCATCCGTTTCTGGAGCTGGCTCCGCTT 300


4124R-2.IR_full       301 CAGTTCCGGACTCTGCCTCTTCTTCAGACAGCACTGATAGCGCCGGAGCACCTGTGACGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     301 CAGTTCCGGACTCTGCCTCTTCTTCAGACAGCACTGATAGCGCCGGAGCACCTGTGACGT 360


4124R-2.IR_full       361 ATACCATCACCTCGGCGGCCAACAGTAGCAATTCCAACAGCATTACCATCAAGTGGAAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     361 ATACCATCACCTCGGCGGCCAACAGTAGCAATTCCAACAGCATTACCATCAAGTGGAAGC 420


4124R-2.IR_full       421 CTTTGTTGGACAACCCAGATGGTGGCGACGAAACGGATTCTGGCACACAATCGAAAGACA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4124R-2.in silico     421 CTTTGTTGGACAACCCAGATGGTGGCGACGAAACGGATTCTGGCACACAATCGAAAGACA 480


4124R-2.IR_full       481 ACGAGGTGGAACATCTGGAA 500
                          ||||||||||||||||||||
4124R-2.in silico     481 ACGAGGTGGAACATCTGGAA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_001014453.1  CG33526-RD, transcript variant D (PNUTS), mRNA 
100   482  0  0  0  NM_001014452.1  CG33526-RC, transcript variant C (PNUTS), mRNA 
100   482  0  0  0  NM_001014455.1  CG33526-RB, transcript variant B (PNUTS), mRNA 
100   482  0  0  0  NM_001014454.1  CG33526-RA, transcript variant A (PNUTS), mRNA 
0   0  5  7  18  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
0   0  5  7  18  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
0   0  3  5  2  NM_164941.1  CG17118-RA (CG17118), mRNA 
0   0  3  3  2  NM_206662.1  CG12065-RC, transcript variant C (CG12065), mRNA 
0   0  3  3  2  NM_167162.2  CG12065-RB, transcript variant B (CG12065), mRNA 
0   0  3  3  2  NM_132265.2  CG12065-RA, transcript variant A (CG12065), mRNA 
0   0  3  3  2  NM_206661.1  CG12065-RD, transcript variant D (CG12065), mRNA 
0   0  2  6  11  NM_080325.3  CG6450-RC (lva), mRNA 
0   0  2  6  2  NM_080071.2  CG12028-RA (dib), mRNA 
0   0  2  3  2  NM_001043289.1  CG34100-RA, transcript variant A (mld), mRNA 
0   0  2  3  2  NM_001043290.1  CG34100-RB, transcript variant B (mld), mRNA 
0   0  2  2  4  NM_132074.1  CG3585-RA (CG3585), mRNA 
0   0  2  2  2  NM_136607.1  CG8078-RA (CG8078), mRNA 
0   0  1  6  5  NM_170027.2  CG31158-RA, transcript variant A (CG31158), mRNA 
0   0  1  6  5  NM_176541.1  CG31158-RB, transcript variant B (CG31158), mRNA 
0   0  1  4  4  NM_138205.1  CG3386-RA (CG3386), mRNA 
0   0  1  4  4  NM_079484.4  CG7437-RA, transcript variant A (mub), mRNA 
0   0  1  4  4  NM_168946.3  CG7437-RB, transcript variant B (mub), mRNA 
0   0  1  4  4  NM_206423.2  CG7437-RC, transcript variant C (mub), mRNA 
0   0  1  4  3  NM_137509.2  CG5482-RA (CG5482), mRNA 
0   0  1  3  8  NM_057943.3  CG12249-RA, transcript variant A (mira), mRNA 
0   0  1  3  8  NM_057944.3  CG12249-RB, transcript variant B (mira), mRNA 
0   0  1  3  5  NM_132259.2  CG11219-RA (PIP82), mRNA 
0   0  1  3  5  NM_143754.2  CG18660-RC, transcript variant C (Nckx30C), mRNA 
0   0  1  3  5  NM_164870.1  CG18660-RB, transcript variant B (Nckx30C), mRNA 
0   0  1  3  5  NM_164869.1  CG18660-RA, transcript variant A (Nckx30C), mRNA 

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